Search results for " virus infection"

showing 10 items of 135 documents

Detection of Torque Teno Virus (TTV) and TTV-Like Minivirus in patients with presumed infectious endophthalmitis in India

2020

Human anelloviruses (Torque Teno Virus (TTV) and TTV Like Mini Virus (TLMV)) are now being reported at a high prevalence across the world, with a controversial disease-inducing potential. The aim of this study was to investigate the role of these anellovirus in vitreous of patients with presumed infectious endophthalmitis. After informed consent, vitreous fluid from patients with endophthalmitis (n = 103) and non-infectious pathologies (n = 102) were analyzed for the presence of TTV and TLMV DNA by qPCR with the limit of quantification defined as 100 copies per reaction. Among the patients clinically diagnosed with endophthalmitis, 29 of the 40 culture proven samples (72.5%) and 42 out of 6…

0301 basic medicineMaleTorque teno virusEye DiseasesGenes ViralPhysiologyAntibioticsInfectious endophthalmitisArtificial Gene Amplification and ExtensionPathology and Laboratory MedicineGastroenterologyPolymerase Chain Reactionlaw.inventionEndophthalmitislawMedicine and Health SciencesChildPolymerase chain reactionAged 80 and overEndophthalmitisMultidisciplinaryVitreous FluidQRMiddle AgedDNA Virus InfectionsBody FluidsAnellovirusesBloodMedical MicrobiologyViral PathogensChild PreschoolVirusesMedicineRetinal DisordersFemaleAnatomyPathogensResearch ArticleAdultmedicine.medical_specialtyAdolescentmedicine.drug_classScience030106 microbiologyIndiaSurgical and Invasive Medical ProceduresResearch and Analysis MethodsMicrobiology03 medical and health sciencesOcular SystemInternal medicinemedicineHumansIn patientMolecular Biology TechniquesMolecular BiologyMicrobial PathogensAgedTorque teno virusBiology and life sciencesbusiness.industryOrganismsRetinal DetachmentInfantmedicine.diseaseOphthalmology030104 developmental biologyAnellovirusesEyesbusinessDNA virusesHeadPLoS ONE
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Epidemiologic and Clinical Characteristics of Coronavirus and Bocavirus Respiratory Infections after Allogeneic Stem Cell Transplantation: A Prospect…

2018

Highlights • Human coronavirus are common after allogeneic stem cell transplantation and can progress to LRTDs, and in some cases this leads to hospitalization and requires supportive care. • Human bocavirus are quite rare after allogeneic stem cell transplantation and are commonly detected in conjunction with other viral co-pathogens.

0301 basic medicineMalevirusesBocavirus Community-acquired respiratory virusmedicine.disease_cause0302 clinical medicineHuman bocavirus030212 general & internal medicineProspective StudiesRespiratory Tract InfectionsCoronavirusRespiratory tract infectionsbiologyHuman bocavirusHematopoietic Stem Cell Transplantationvirus diseasesHematologyMiddle AgedAllograftsHospitalizationViral pneumoniaRespiratory virusFemaleCoronavirus InfectionsAdultmedicine.medical_specialtyViral pneumonia030106 microbiologyContext (language use)ArticleParvoviridae InfectionsBocavirus03 medical and health sciencesCommunity-acquired respiratory virusInternal medicinemedicineHumansAgedTransplantationbusiness.industrymedicine.diseasebiology.organism_classificationVirologyrespiratory tract diseasesAllogeneic stem cell transplantationTransplantationCoronavirusRespiratory failureRespiratory virus infectionbusinessFollow-Up Studies
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Comparison of the artus Epstein-Barr virus (EBV) PCR kit and the Abbott RealTime EBV assay for measuring plasma EBV DNA loads in allogeneic stem cell…

2017

The ability of the artus Epstein-Barr virus (EBV) PCR kit and the Abbott RealTime EBV PCR assay to detect and quantify plasma EBV DNAemia was compared. The agreement between these assays was 95.8%. The EBV DNA loads measured by the two assays significantly correlated (P=< 0.0001).

0301 basic medicineMicrobiology (medical)AdultEpstein-Barr Virus InfectionsHerpesvirus 4 Human030106 microbiologyPcr assayBiologymedicine.disease_causeVirus03 medical and health sciencesPlasmahemic and lymphatic diseasesmedicineHumansTransplantation HomologousGeneral MedicineViral LoadEpstein–Barr virusVirologyTransplant Recipients030104 developmental biologyInfectious DiseasesReal-time polymerase chain reactionMolecular Diagnostic TechniquesDNA ViralStem cellStem Cell TransplantationDiagnostic microbiology and infectious disease
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Assessment of prevalence and load of torquetenovirus viraemia in a large cohort of healthy blood donors.

2020

OBJECTIVES: Torquetenovirus (TTV) is an emerging marker of functional immune competence with the potential to predict transplant-related adverse events. A large-scale epidemiological study was performed to understand how basal values vary in healthy individuals according to age and gender.; METHODS: We tested plasma from 1017 healthy blood donors aged 18-69years. The presence and load of TTV were determined by a real-time PCR assay. A sub-cohort of 384 donors was tested for anti-cytomegalovirus IgG antibodies, and 100 participants were also tested for TTV viraemia on a paired whole blood sample.; RESULTS: The overall prevalence of TTV was 65% (657/1017) with a mean (±SD) growth of 5±4% ever…

0301 basic medicineMicrobiology (medical)AdultMalemedicine.medical_specialtyAgingAdolescentprevalence030106 microbiologyPcr assayPhysiologyTTVViremiaBlood DonorsanelloviridaeReal-Time Polymerase Chain Reaction03 medical and health sciencesPlasmaYoung Adult0302 clinical medicineEpidemiologyMedicineHumansBlood Transfusion030212 general & internal medicineViremiaTTV; anelloviridae; blood donors; healthy controls; prevalence; torquetenovirus; viremiaAdverse effectWhole bloodAgedTorque teno virusbiologybusiness.industryGeneral MedicineMiddle AgedViral Loadmedicine.diseaseDNA Virus InfectionsHealthy VolunteersLarge cohorttorquetenovirusInfectious DiseasesHealthy individualsDNA Viralbiology.proteinhealthy controlsFemaleAntibodybusinessClinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
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Helicobacter pylori and Epstein–Barr Virus Infection in Gastric Diseases: Correlation with IL-10 and IL1RN Polymorphism

2019

Introduction. Helicobacter pylori and Epstein–Barr virus (EBV) infection have recently been shown to be associated with gastric diseases. Polymorphisms in genes encoding cytokines such as interleukin 10 (IL-10) and interleukin 1 Receptor (IL-1RN) influence cytokine secretion levels and appear to contribute to the risk of developing gastroduodenal diseases. To our knowledge, this is the first preliminary study to address the association of coinfection with H. pylori and EBV and their correlation with genetic predisposition in the development of gastric diseases. Methods. Gastric biopsy samples of 96 patients with different gastric diseases were used. Results. Our results showed that the rate…

0301 basic medicineSettore MED/07 - Microbiologia E Microbiologia ClinicaArticle Subjectpolymorphism gastric cancer IL-10Chronic gastritislcsh:RC254-28203 medical and health sciences0302 clinical medicineEBVHelicobactermedicineCagAEpstein–Barr virus infectionbiologybusiness.industryMALT lymphomaHelicobacter pylorimedicine.diseasebiology.organism_classificationlcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogensdigestive system diseases030104 developmental biologyOncology030220 oncology & carcinogenesisImmunologyCoinfectionCytokine secretionGastritismedicine.symptombusinessResearch ArticleJournal of Oncology
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ISG15 Is Upregulated in Respiratory Syncytial Virus Infection and Reduces Virus Growth through Protein ISGylation

2016

ABSTRACT Human respiratory syncytial virus (RSV), for which neither a vaccine nor an effective therapeutic treatment is currently available, is the leading cause of severe lower respiratory tract infections in children. Interferon-stimulated gene 15 (ISG15) is a ubiquitin-like protein that is highly increased during viral infections and has been reported to have an antiviral or a proviral activity, depending on the virus. Previous studies from our laboratory demonstrated strong ISG15 upregulation during RSV infection in vitro . In this study, an in-depth analysis of the role of ISG15 in RSV infection is presented. ISG15 overexpression and small interfering RNA (siRNA)-silencing experiments,…

0301 basic medicineSmall interfering RNAvirusesImmunologyCellular Response to InfectionRespiratory Syncytial Virus InfectionsUbiquitin-Activating EnzymesBiologyMicrobiologyVirus03 medical and health sciencesIn vivoImmunityRNA interferenceVirologyCell Line TumorEndopeptidasesHumansRNA Small InterferingRespiratory Tract InfectionsUbiquitinsInnate immune system030102 biochemistry & molecular biologyRespiratory tract infectionsInfantEpithelial CellsISG15VirologyImmunity Innate030104 developmental biologyInsect ScienceRespiratory Syncytial Virus HumanCytokinesRNA InterferenceUbiquitin ThiolesteraseProtein Processing Post-TranslationalHeLa Cells
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The Antiviral Properties of Cyclosporine. Focus on Coronavirus, Hepatitis C Virus, Influenza Virus, and Human Immunodeficiency Virus Infections

2020

This review updates current knowledge regarding the risk of viral infections, including COVID-19, in patients treated with cyclosporine. We also shortly refer to bacterial infections and parasitic infestations in patients treated with cyclosporin. Cyclosporine is an immunosuppressive drug, which is widely used in medicine, including in the treatment of autoimmune skin diseases in dermatology, rheumatology, ophthalmology and nephrology, and in organ transplantation. A usual concern associated with immunosuppressive treatment is the potential risk of infections. Interestingly, several data indicate a relatively low risk of infections, especially viral infections, in patients receiving cyclosp…

0301 basic medicinemedicine.medical_specialtyvirusesmedicine.medical_treatmentHepatitis C viruscoronavirusReviewBiologymedicine.disease_causeGeneral Biochemistry Genetics and Molecular BiologyVirusOrgan transplantation030207 dermatology & venereal diseases03 medical and health sciences0302 clinical medicineRotavirusmedicinecyclosporineinfectionstacrolimuslcsh:QH301-705.5Coronavirushuman papilloma virus infectionGeneral Immunology and MicrobiologyHepatitis Cmedicine.diseasecalcineurin inhibitorshuman herpesvirusVirologyTacrolimusAIDShepatitis flu030104 developmental biologyImmunosuppressive druglcsh:Biology (General)cyclophilinGeneral Agricultural and Biological SciencesBiology
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Subnational mapping of HIV incidence and mortality among individuals aged 15-49 years in sub-Saharan Africa, 2000-18: a modelling study

2021

Background. High-resolution estimates of HIV burden across space and time provide an important tool for tracking and monitoring the progress of prevention and control efforts and assist with improving the precision and efficiency of targeting efforts. We aimed to assess HIV incidence and HIV mortality for all second-level administrative units across sub-Saharan Africa. Methods. In this modelling study, we developed a framework that used the geographically specific HIV prevalence data collected in seroprevalence surveys and antenatal care clinics to train a model that estimates HIV incidence and mortality among individuals aged 15–49 years. We used a model-based geostatistical framework to e…

0301 basic medicinesub-Saharan AfricaMaleHIV AntibodieEpidemiologyHIV incidencesHIV Infectionsmortality rateHIV AntibodiesModelling studyHuman immunodeficiency virus prevalence0302 clinical medicineAfrica NorthernRA0421Seroepidemiologic StudiesMedicineNorthernHIV Infection030212 general & internal medicineYoung adult10. No inequalityuncertaintyMozambique11 Medical and Health SciencesHIV mortalityMortality rateIncidence (epidemiology)Incidence1. No povertyHiv incidencearticleMauritaniaHuman immunodeficiency virus infected patientArticlestrackingMiddle Agedhealth care planning3. Good healthPeer reviewLesothoAIDSInfectious DiseasesQR180A990 Medicine and Dentistry not elsewhere classifiedFemaleprenatal careanti human immunodeficiency virus agentseroepidemiologyHumanAdultAdolescentAnti-HIV AgentsImmunologyantiretroviral therapyUnit (housing)03 medical and health sciencesYoung AdultbloodHuman immunodeficiency virus infectionVirologySeroprevalenceHumanshumanDeveloping CountriesEstimationAcquired Immunodeficiency SyndromeSubnational mappingsbusiness.industrySeroepidemiologic StudieHIVAnti-HIV AgentPREVENTION030112 virologymortalitymonitoring3121 General medicine internal medicine and other clinical medicineHuman immunodeficiency virus antibodyAfricabusinessHIV incidence ; HIV mortality ; Subnational mapping ; Sub-Saharan Africa ; Local burden of disease ; Public healthDemography
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Constrained evolvability of interferon suppression in an RNA virus.

2016

AbstractInnate immunity responses controlled by interferon (IFN) are believed to constitute a major selective pressure shaping viral evolution. Viruses encode a variety of IFN suppressors, but these are often multifunctional proteins that also play essential roles in other steps of the viral infection cycle, possibly limiting their evolvability. Here, we experimentally evolved a vesicular stomatitis virus (VSV) mutant carrying a defect in the matrix protein (M∆51) that abolishes IFN suppression and that has been previously used in the context of oncolytic virotherapy. Serial transfers of this virus in normal, IFN-secreting cells led to a modest recovery of IFN blocking capacity and to weak …

0301 basic medicineviruses030106 microbiologyAdaptation BiologicalBiologyVirus ReplicationModels BiologicalVirusArticleCell Line03 medical and health sciencesViral ProteinsRNA Virus InfectionsInterferonmedicineHumansRNA VirusesPhosphorylationMultidisciplinaryViral matrix proteinInterferon SuppressionGenetic Variationbiology.organism_classificationVirologyBiological EvolutionImmunity InnateOncolytic virus030104 developmental biologyViral replicationVesicular stomatitis virusViral evolutionMutationInterferonsmedicine.drugScientific reports
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Lower ST-elevation myocardial infarction incidence during COVID-19 epidemic in Northern Europe

2020

We compared the ST elevation myocardial infarction (STEMI) incidence during COVID-19 pandemic (March 2020) to January-February 2020 and to same time period in earlier years 2017-2019 in five Nordic-Baltic tertiary centers. During 2017-2019, there were no marked differences in STEMI incidence between January, February and March. During 2020, there was an average drop of 32% in STEMI incidence in March. The isolation measures may decrease the risk for respiratory virus infection and contribute to the lower STEMI incidence and that we might benefit from firmer suggestions on hand hygiene and social distancing during flu season at least among high-risk individuals.

2019-20 coronavirus outbreakCoronavirus disease 2019 (COVID-19)media_common.quotation_subject030204 cardiovascular system & hematologyScandinavian and Nordic CountriesTertiary Care Centers03 medical and health sciences0302 clinical medicineSt elevation myocardial infarctionHygieneRespiratory virus infectionPandemicFlu seasonMedicineHumanscardiovascular diseases030212 general & internal medicinemedia_commonRetrospective Studiesbusiness.industryIncidence (epidemiology)IncidenceCOVID-19Latvia3. Good healthsurgical procedures operativeST-elevation myocardial infarctionincidenceST Elevation Myocardial InfarctionbusinessCardiology and Cardiovascular MedicineDemography
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