Search results for " vivo"

MP-03.04 The Combination Treatment of Bicalutamide Plus Carbidopa Significantly Enhances the in vivo Antitumor Activity on LNCaP Castration-resistant…

2011

Sulphoevernan, a polyanionic polysaccharide, and the narcissus lectin potently inhibit human immunodeficiency virus infection by binding to viral env…

1990

Sulphoevernan is a sulphated alpha-1----3, 1----4 polyglucan (Mr 20,000) with a helical structure. This compound effectively inhibits both human immunodeficiency virus type 1 (HIV-1) and type 2 infection of cells in vitro at concentrations around 0.5 micrograms/ml. Moreover, the compound completely inhibits HIV-1-induced syncytium formation at a concentration of 1 microgram/ml. Competition experiments with 35S-labelled sulphoevernan revealed that the mannose-specific lectin from Narcissus pseudonarcissus prevented binding of sulphoevernan to HIV-1, whereas the antibody OKT4A did not reduce the amount of sulphoevernan bound to MT-2 cells. These data indicate that the non-cytotoxic polymer su…

Arterial and mixed venous blood gas status during apnoea of intubation--proof of the Christiansen-Douglas-Haldane effect in vivo.

1989

The Christiansen-Douglas-Haldane effect, in short the Haldane effect, describes the dependence of the CO2 binding of blood on the degree of oxygenation of haemoglobin. Under the physiological conditions of an ‘open’ system between blood and alveoli the partial pressure of arterial C02 (PaCO2), must be less than that of mixed venous blood (P[Formula: see text]CO2). During the unphysiological conditions of a ‘closed’ system, e.g. hyperoxic apnoea, i.e. continuous oxygen uptake without CO2 delivery by the lungs, the Paco2 will not only approximate the P[Formula: see text]CO2 but will even exceed it. Without the Haldane effect, rapid adjustment of Paco2 to P[Formula: see text]CO2 would be expe…

New hydrogel matrices based on chemical crosslinked α,β -polyas parthydrazide: Synthesis, characterization and in vivo biocompatibility studies

1996

New swellable micromatrices of α,β-polyasparthydrazide (PAHy) crosslinked with glutaraldehyde were prepared. The effect of crosslinking agent concentration was evaluated. In particular, crosslinking density affected aqueous dynamic swelling and glass-transition temperature of the material. The structure of prepared networks was also studied by scanning electron microscopy and X-ray analysis. Finally, biocompatibility of PAHy derivatives was investigated in vivo by subcutaneous implantation and in oral administration to laboratory animals.

A modelistic approach showing the importance of the stagnant aqueous layers in in vitro diffusion studies, and in vitro-in vivo correlations

1991

Abstract The present study deals with the role of the aqueous diffusion layers on the in vitro penetration of xenobiotics across artificial lipoidal membranes, and their ability to reproduce biophysical absorption models when in vivo results are to be simulated from the in vitro tests. The aqueous boundary layers which are invariably formed on artificial lipoidal membranes can be optionally preserved or disrupted, according to the type of absorption site which should be simulated, a condition which could reasonably lead to a better correspondence between in vitro and in vivo results; in practice, disruption of water layers can be easily achieved by a synthetic surfactant solution at its cri…

The anti-inflammatory fungal compound (S)-curvularin reduces proinflammatory gene expression in an in vivo model of rheumatoid arthritis.

2012

In previous studies, we identified the fungal macrocyclic lactone (S)-curvularin (SC) as an anti-inflammatory agent using a screening system detecting inhibitors of the Janus kinase/signal transducer and activator of transcription pathway. The objective of the present study was to investigate whether SC is able to decrease proinflammatory gene expression in an in vivo model of a chronic inflammatory disease. Therefore, the effects of SC and dexamethasone were compared in the model of collagen-induced arthritis (CIA) in mice. Total genomic microarray analyses were performed to identify SC target genes. In addition, in human C28/I2 chondrocytes and MonoMac6 monocytes, the effect of SC on proi…

EMG activity of pigeon oesophagus in vivo.

1982

At rest, the pigeon cervical oesophagus, which is entirely smooth muscle, shows electric activity. This activity consists of bursts of spikes with frequency increasing in the oral-aboral direction. The bursts are un-phase locked, and there are no slow waves (E.C.A.). The surgical transection of the oesophageal muscular wall does not affect the electric activity even in a disconnected segment. After asphyxia electric activity persists, whereas the aboral gradient of frequency disappears. Therefore, the electric activity is thought to be myogenic in origin, and the frequency gradient nervous in origin. Atropine and neostigmine administration suggests that the cholinergic system modulates the …

Parallel Measurements of in-vivo Skin Autofluorescence Lifetimes and Photobleaching Rates

2015

Experimental methodology for parallel measurements of in-vivo skin autofluorescence (AF) lifetimes and photo-bleaching dynamic has been developed and tested. The AF lifetime decay distributions were periodically collected from fixed tissue area with subsequent detection of the fluorescence intensity decrease dynamic at different time shifts after the pulse excitation. Temporal distributions of skin AF lifetimes and bleaching dynamic were collected and analyzed by means of commercial time-correlated single photon counting system. Details of the equipment and data processing are described as well as some measurement results that confirm the feasibility of the proposed technology.

Photobleaching measurements of pigmented and vascular skin lesions: results of a clinical trial

2011

The autofluorescence photobleaching intensity dynamics of in vivo skin and skin pathologies under continuous 532 nm laser irradiation have been studied. Overall the 141 human skin malformations were investigated by laser induced skin autofluorescence photobleaching analysis. Details of equipment are described along with some measurement results illustrating potentiality of the technology.

Platelet-neutrophil aggregates promote skin pathology in psoriasis

2019

AbstractPsoriasis is a frequent systemic inflammatory autoimmune disease characterized primarily by skin lesions with massive infiltration of leukocytes but frequently also presents with cardiovascular comorbidities. Especially polymorphonuclear neutrophils (PMNs) abundantly infiltrate psoriatic skin but the cues that prompt PMNs to home to the skin are not well defined. To identify PMN surface receptors that may explain PMN skin homing in psoriasis patients, we screened 332 surface antigens on primary human blood PMNs from healthy donors and psoriasis patients. We identified platelet surface antigens as a defining feature of psoriasis PMNs, due to a significantly increased aggregation of n…