Search results for "46"
showing 10 items of 1176 documents
Correlative Raman-Electron-Light (CREL) Microscopy Analysis of Lipid Droplets in Melanoma Cancer Stem Cells.
2022
Among all neoplasms, melanoma is characterized by a very high percentage of cancer stem cells (CSCs). Several markers have been proposed for their identification, and lipid droplets (LDs) are among them. Different techniques are used for their characterization such as mass spectrometry, imaging techniques, and vibrational spectroscopies. Some emerging experimental approaches for the study of LDs are represented by correlative light–electron microscopy and by correlative Raman imaging–scanning electron microscopy (SEM). Based on these scientific approaches, we developed a novel methodology (CREL) by combining Raman micro-spectroscopy, confocal fluorescence microscopy, and SEM coupled with an…
Absence of mutations in the WT1 gene in patients with XY gonadal dysgenesis
1995
The WT1 gene is normally expressed during gonadal development and specific mutations in heterozygous form cause Drash syndrome, characterized by male pseudohermaphroditism and gonadal dysgenesis, renal failure and a predisposition for Wilms' tumour. These observations prompted us to test whether WT1 mutations are involved in isolated gonadal dysgenesis, being the most severe form of disturbance in gonadal differentiation. We studied 27 cases of 46,XY females with gonadal dysgenesis who had previously been screened for and found not to carry SRY gene mutations. We performed mutational screening of the WT1 gene with denaturing gradient gel electrophoresis. In one of these patients, a heterozy…
Analysis of Social Media Impact on Opportunity Recognition. A Social Networks and Entrepreneurial Alertness Mixed Approach
2020
This paper investigates the effects of social media on entrepreneurial opportunity recognition. Combining the internal and external approaches of opportunity recognition, the study analyzes how social media influences the entrepreneurs in discovering new entrepreneurial opportunities. Structural equation modeling was used in this study, using the variance-based partial least squares (PLS)&ndash
Complejos metálicos biestables para la electrónica molecular: síntesis y organización sobre superficies de rotaxanos y moléculas imán
2008
En Capítulo 1 se aborda la síntesis de un nuevo sistema biestable basado en un rotaxano coordinado de cobre, incorporando en el fragmento lineal unidades de terpiridina y fenantrolina directamente enlazadas por sus posiciones T5 y F3 así como los intentos de utilizarlo en la construcción de un rotaxano biestable. En el Capítulo 2 se demuestra la posibilidad de utilizar un conjugado de 3,5-fenantrolina terpiridina (L) en la construcción de diferentes sistemas de interés. En una primera parte, se presenta la síntesis una serie de nuevos complejos luminiscentes mono-, di- y trinucleares basados en Ru/Os, estudiándose sus propiedades de absorción y emisión, así como los tiempos de vida del esta…
Fractional Maximal Functions in Metric Measure Spaces
2013
Abstract We study the mapping properties of fractional maximal operators in Sobolev and Campanato spaces in metric measure spaces. We show that, under certain restrictions on the underlying metric measure space, fractional maximal operators improve the Sobolev regularity of functions and map functions in Campanato spaces to Hölder continuous functions. We also give an example of a space where fractional maximal function of a Lipschitz function fails to be continuous.
Sobolev homeomorphic extensions onto John domains
2020
Given the planar unit disk as the source and a Jordan domain as the target, we study the problem of extending a given boundary homeomorphism as a Sobolev homeomorphism. For general targets, this Sobolev variant of the classical Jordan-Schoenflies theorem may admit no solution - it is possible to have a boundary homeomorphism which admits a continuous $W^{1,2}$-extension but not even a homeomorphic $W^{1,1}$-extension. We prove that if the target is assumed to be a John disk, then any boundary homeomorphism from the unit circle admits a Sobolev homeomorphic extension for all exponents $p<2$. John disks, being one sided quasidisks, are of fundamental importance in Geometric Function Theory.
Galveno interešu centra noteikšana pārrobežu maksātnespējas procedūrās - tiesu prakses analīze.
2020
Maģistra darbā “Galveno interešu centra noteikšana pārrobežu maksātnespējas procedūrās – tiesu prakses analīze” pētīta tiesu prakses nozīme maksātnespējas procedūrās, kurās piemēro Padomes Regulas (EK) Nr. 1346/2000 (2000.gada 29.maijs) par maksātnespējas procedūrām un Eiropas Parlamenta un Padomes Regula (ES) 2015/848 (2015.gada 20.maijs) par maksātnespējas procedūrām normas. Darbā pētīta Latvijas tiesu un Eiropas Savienības Tiesas prakse. Pētījuma mērķis: noskaidrot, vai Latvijas tiesu prakse ir atbilstoša Eiropas Parlamenta un Padomes Regulas (ES) 2015/848 par maksātnespējas procedūrām normām un pašreizējai Eiropas Savienības Tiesas praksei. Pētījuma uzdevumi: izpētīt pārrobežu maksātnes…
Changes of the eye optics after iris constriction☆
2010
Purpose: To evaluate the possible change in the optics of the human eye after iris constriction. Methods: Ocular aberrations were measured under natural viewing conditions in 26 eyes. The measured eyes fixated on a dim target while the contralateral eye was either occluded (so the measured eye had a large pupil) or highly illuminated (so the measured eye had a small pupil). The measured eyes fixated to a dim target placed 0.5 D beyond the subject’s far point. Zernike values obtained in both situations were compared within the same pupil diameter corresponding to the one obtained under the high illumination condition. Results: Significant variation in some aberration coefficients were found …
Social Cognition in Neurodevelopmental Disorders and Epilepsy
2021
Introduction: The purpose of our study was to perform a comparative analysis of social cognition in children and adolescents with epilepsy, autism spectrum disorder (ASD), specific learning disorder (SLD) and in typical development (TD) controls. The secondary aim was to relate social cognition to some clinical and demographic characteristics.Methods: Our work is a transversal observational study. The recruits were 179 children and adolescents aged between 6 and 18 years diagnosed with epilepsy, ASD, or SLD and 32 subjects with TD. All the participants underwent neuropsychological assessment of Emotion Recognition (ER) and Theory of Mind (ToM) skills.Results: All three clinical groups perfo…
In vivo consequences of cholesterol-24s-hydroxylase (CYP46A1) inhibition by voriconazole on cholesterol homeostasis and function in the rat retina
2011
Cholesterol 24S-hydroxylase (CYP46A1) converts cholesterol into 24S-hydroxycholesterol in neurons and participates in cholesterol homeostasis in the central nervous system, including the retina. We aimed to evaluate the consequences of CYP46A1 inhibition by voriconazole on cholesterol homeostasis and function in the retina. Rats received daily intraperitoneal injections of voriconazole (60mg/kg), minocycline (22mg/kg), voriconazole plus minocycline, or vehicle during five consecutive days. The rats were submitted to electroretinography to monitor retinal functionality. Cholesterol and 24S-hydroxycholesterol were measured in plasma, brain and retina by gas chromatography-mass spectrometry. T…