Search results for "48"
showing 10 items of 1388 documents
Microbial communities of the Mediterranean rocky shore: ecology and biotechnological potential of the sea‐land transition
2019
Microbial communities from harsh environments hold great promise as sources of biotechnologically relevant strains and compounds. In the present work, we have characterized the microorganisms from the supralittoral and splash zone in three different rocky locations of the Western Mediterranean coast, a tough environment characterized by high levels of irradiation and large temperature and salinity fluctuations. We have retrieved a complete view of the ecology and functional aspects of these communities and assessed the biotechnological potential of the cultivable microorganisms. All three locations displayed very similar taxonomic profiles, with the genus Rubrobacter and the families Xenoco…
Detection of Candidatus Neoehrlichia mikurensis in Norway up to the northern limit of Ixodes ricinus distribution using a novel real time PCR test ta…
2019
Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Background Candidatus Neoehrlichia mikurensis is an emerging tick-borne pathogen. It is widely distributed in Ixodes ricinus ticks in Europe, but knowledge of its distribution in Norway, where I. ricinus reaches its northern limit, is limited. In this study we have developed a real time PCR test for Ca. N. mikurensis and used it to invest…
Intervención cognitivo conductual para mujeres con trastorno depresivo persistente: sintomatología ansiosa, depresiva y satisfacción vital
2021
Antecedentes. El trastorno depresivo persistente (TDP) es una afectación del estado de ánimo frecuente en mujeres. Se ha empleado la terapia cognitivo conductual (TCC), la terapia cognitiva basada en mindfulness y la terapia centrada en la compasión de forma aislada. Objetivos. Evaluar la eficacia de un programa de intervención grupal multicomponente. Método. Tras la firma del consentimiento informado, las participantes (n = 6) respondieron (pre, post intervención y seguimiento) cuestionarios sobre: ansiedad, depresión, satisfacción con la vida y satisfacción con la intervención. Resultados. Nuestros resultados previos al tratamiento muestran niveles elevados de sintomatología ansiosa y dep…
The liquid state of FG-nucleoporins mimics permeability barrier properties of nuclear pore complexes
2019
Nuclear pore complexes form a permeability barrier in vivo that regulates nucleocytoplasmic transport. Here, the authors present a microfluidic device that couples rapid liquid–liquid phase separation of nucleoporins with direct optical interrogation. Freshly formed liquid nucleoporin droplets mimic permeability barrier properties of NPCs.
Toward fast malaria detection by secondary speckle sensing microscopy
2012
Diagnosis of malaria must be rapid, accurate, simple to use, portable and low cost, as suggested by the World Health Organization (WHO). Despite recent efforts, the gold standard remains the light microscopy of a stained blood film. This method can detect low parasitemia and identify different species of Plasmodium. However, it is time consuming, it requires well trained microscopist and good instrumentation to minimize misinterpretation, thus the costs are considerable. Moreover, the equipment cannot be easily transported and installed. In this paper we propose a new technique named "secondary speckle sensing microscopy" ((SM)-M-3) based upon extraction of correlation based statistics of s…
Mitochondrial genome sequence and gene order of Sipunculus nudus give additional support for an inclusion of Sipuncula into Annelida.
2010
Abstract Background Mitochondrial genomes are a valuable source of data for analysing phylogenetic relationships. Besides sequence information, mitochondrial gene order may add phylogenetically useful information, too. Sipuncula are unsegmented marine worms, traditionally placed in their own phylum. Recent molecular and morphological findings suggest a close affinity to the segmented Annelida. Results The first complete mitochondrial genome of a member of Sipuncula, Sipunculus nudus, is presented. All 37 genes characteristic for metazoan mtDNA were detected and are encoded on the same strand. The mitochondrial gene order (protein-coding and ribosomal RNA genes) resembles that of annelids, b…
Louisa S. McCord's Caius Gracchus: A Transatlantic Southern Literary Response to 1848 European Revolutions
2016
Louisa S. McCord is the most important female intellectual in the antebellum South and one of its most recognized voices, even if her name rarely appears in studies not directly related to her region. McCord has a parallel, according to Mary Kelley, in Margaret Fuller, the relevant prewar Northern intellectual and essayist. From contrasting ideological positions, both left testimony of their interest in constructing a model of womanhood, capable of facing the contingencies of their times. Born in one of the most influential families of South Carolina, McCord produced a phenomenal synthesis of the conservative political, economic and religious arguments accepted in her times and managed to t…
Nitric oxide inhibits the ATPase activity of the chaperone-like AAA+ ATPase CDC48, a target for S-nitrosylation in cryptogein signalling in tobacco c…
2012
NO has important physiological functions in plants, including the adaptative response to pathogen attack. We previously demonstrated that cryptogein, an elicitor of defence reaction produced by the oomycete Phytophthora cryptogea , triggers NO synthesis in tobacco. To decipher the role of NO in tobacco cells elicited by cryptogein, in the present study we performed a proteomic approach in order to identify proteins undergoing S-nitrosylation. We provided evidence that cryptogein induced the S-nitrosylation of several proteins and identified 11 candidates, including CDC48 (cell division cycle 48), a member of the AAA+ ATPase (ATPase associated with various cellular activities) family. In vit…
Detection of Hypoxanthine from Inosine and Unusual Hydrolysis of Immunosuppressive Drug Azathioprine through the Formation of a Diruthenium(III) Syst…
2021
Hypoxanthine (hpx) is an important molecule for both biochemistry research and biomedical applications. It is involved in several biological processes associated to energy and purine metabolism and has been proposed as a biomarker for a variety of disease states. Consequently, the discovery and development of systems suitable for the detection of hypoxanthine is pretty appealing in this research field. Thus, we have obtained a stable diruthenium (III) compound in its dehydrated and hydrated forms with formula [{Ru(µ
Spherical ordered mesoporous silicas and silica monoliths as stationary phases for liquid chromatography
2006
Ordered mesoporous silicas such as micelle-templated silicas (MTS) feature unique textural properties in addition to their high surface area (approximately 1000 m2/g): narrow mesopore size distributions and controlled pore connectivity. These characteristics are highly relevant to chromatographic applications for resistance to mass transfer, which has never been studied in chromatography because of the absence of model materials such as MTS. Their synthesis is based on unique self-assembly processes between surfactants and silica. In order to take advantage of the perfectly adjustable texture of MTS in chromatographic applications, their particle morphology has to be tailored at the microme…