The liquid state of FG-nucleoporins mimics permeability barrier properties of nuclear pore complexes

6533b7dbfe1ef96bd126f785

RESEARCH PRODUCT

The liquid state of FG-nucleoporins mimics permeability barrier properties of nuclear pore complexes

Virginia Vandelinder Giorgia Celetti Joana Caria Giulia Paci George D. Bachand Edward A. Lemke

subject

Microfluidics Active Transport Cell Nucleus 48 Biology Permeability 23 03 medical and health sciences 0302 clinical medicine Report medicine Molecule Nuclear pore Research Articles 030304 developmental biology 0303 health sciences 36 Cell Biology 34 Nuclear Pore Complex Proteins Cell nucleus medicine.anatomical_structure Permeability (electromagnetism)Nucleocytoplasmic Transport Biophysics Nuclear Pore Nucleoporin Nuclear transport 030217 neurology & neurosurgery

description

Nuclear pore complexes form a permeability barrier in vivo that regulates nucleocytoplasmic transport. Here, the authors present a microfluidic device that couples rapid liquid–liquid phase separation of nucleoporins with direct optical interrogation. Freshly formed liquid nucleoporin droplets mimic permeability barrier properties of NPCs.

year	journal	country	edition	language
2019-11-13	The Journal of Cell Biology

10.1083/jcb.201907157 http://europepmc.org/articles/PMC7039189

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