Search results for "AGLA"
showing 10 items of 272 documents
The CO-releasing molecule CORM-2 is a novel regulator of the inflammatory process in osteoarthritic chondrocytes
2008
Previous work has shown that the CO-releasing molecule CORM-2 protects against cartilage degradation. The aim of this study was to examine whether CORM-2 can control the production of inflammatory mediators in osteoarthritic chondrocytes and determine the mechanisms involved.Primary cultures of chondrocytes from OA patients were stimulated with IL-1beta. The production of reactive oxygen species, nitrite, PGE(2), TNF-alpha and IL-1 receptor antagonist (IL-1Ra) were measured in the presence or absence of CORM-2. The expression of nitric oxide synthase-2 (NOS-2), cyclo-oxygenase-2 (COX-2) and microsomal PG E synthase-1 (mPGES-1) was followed by western blot and real-time PCR. Activation of nu…
Protection against 2,4,6-trinitrobenzenesulphonic acid-induced colonic inflammation in mice by the marine products bolinaquinone and petrosaspongioli…
2005
Proinflammatory mediators, namely eicosanoids, reactive oxygen and nitrogen species and cytokines, are clearly involved in the pathogenesis of intestinal bowel disease. bolinaquinone (BQ) and petrosaspongiolide M (PT), two marine products with potent anti-inflammatory action, have been shown to control the production of mediators in acute and chronic inflammatory processes. Hence, we have tested here the hypothesis that BQ and PT could ameliorate inflammation and oxidative stress parameters in 2,4,6-trinitrobenzenesulphonic acid (TNBS)-induced colitis in Balb/c mice. BQ and PT were given orally in doses of 10 or 20mg/kg/day. Treatment of the animals with BQ or PT at the highest dose signifi…
Toll-like receptor 2 mediates prostaglandin E2 production in murine peritoneal macrophages and splenocytes in response to Candida albicans
2004
The involvement of Toll-like receptor 2 (TLR2) and TLR4 in triggering signal transduction pathways leading to prostaglandin E(2) (PGE(2)) production in response to Candida albicans has been studied in cells from wild-type, TLR2-/- and TLR4-/- knockout mice. In vitro PGE(2) production by macrophages challenged with zymosan, yeast or hypha cells was strongly inhibited in TLR2-deficient cells, but not in TLR4-/- cells, as compared to macrophages from wild-type mice. PGE(2) production was dependent on de novo cyclooxygenase-2 (Cox2) synthesis, since unchallenged cells failed to produce PGE(2) and specific Cox2 inhibition during challenge totally blocked PGE(2) production. Similar results were o…
Hamiltona sistēmu risināšanas un mācīšanās struktūru saglabājošie algoritmi
2021
Darbā aplūkotas Hamiltona sistēmu īpašības un struktūru saglabājošie algoritmi, kurus pielietojot tiek saglabātas attiecīgās īpašības. Izrādās, ka svarīga īpašība, kas raksturo Hamiltona sistēmas, ir plūsmas simplektiskums. Tiek apskatītas simplektiskas skaitliskās metodes un tiek pārbaudīts, ka tās darbojas labāk nekā parastas skaitliskās metodes, kad tiek risināta Hamiltona sistēma. Liela uzmanība veltīta neironu tīkliem, izpētot tos un ar tiem saistītās lietas, jo neironu tīklus tagad ir iespējams pielietot arī Hamiltona sistēmām. Tiek aplūkoti uz datiem balstīti simplektiskie neironu tīkli un pielietoti dažādiem piemēriem, pārbaudot to darbības spējas. Praktiskie rezultāti parāda, ka la…
Cytotoxicity of Root Canal Filling Materials to Three Different Human Cell Lines
2001
The aim of this study was to investigate the biological compatibility of five root canal sealers (Sealapex, Endion, Super-EBA, Ketac-Endo, and AH Plus) and regular and calcium hydroxide-based gutta-percha in three different human cell lines. Cultures without root canal sealers were used as controls. Cell growth, cell morphology, cell viability, protein content of the cells, and prostaglandin E 2 (PGE 2 ) release were used as parameters to determine the cytotoxicity of the materials. The protein content of the three cell lines—nasal fibroblasts, gingival fibroblasts, and epithelial tumor cells—was significantly reduced (p ≤ 0.001) by all materials tested. Determinations of PGE 2 release show…
Immobilization and controlled release of prostaglandin E2 from poly-L-lactide-co-glycolide microspheres.
2009
Prostaglandin E(2) (PGE(2)) is an arachidonic acid metabolite involved in physiological homeostasis and numerous pathophysiological conditions. Furthermore, it has been demonstrated that prostaglandins have a stimulating effect not only on angiogenesis in situ and in vitro but also on chondrocyte proliferation in vitro. Thus, PGE(2) represents an interesting signaling molecule for various tissue engineering strategies. However, under physiological conditions, PGE(2) has a half-life time of only 10 min, which limits its use in biomedical applications. In the present study, we investigated if the incorporation of PGE(2) into biodegradable poly-L-lactide-co-glycolide microspheres results in a …
Shallow-water benthic hydroids from the Maltese Islands (Central Mediterranean)
2013
Forty species of benthic hydroids, belonging to 15 families and 21 genera, were found in a collection, obtained by SCUBA diving, from the Maltese Islands. Paradoxically for the well studied Mediterranean region, the hydroid fauna of these islands is virtually unexplored. Of 40 species, 33 were identified to species level, with 28 of them reported for the first time for the study area. Hydractinia monocarpa is recorded for the first time in the Mediterranean Sea, and a presumed new species of the genus Clytia has been found. The most speciose families are Campanulariidae with 10 species (25%), Aglaopheniidae with six species (15%) and Sertulariidae with five species (12%). Aglaophenia and Cl…
Influence of heme oxygenase 1 modulation on the progression of murine collagen-induced arthritis.
2005
Contains fulltext : 48023.pdf (Publisher’s version ) (Closed access) OBJECTIVE: Heme oxygenase 1 (HO-1) can be induced by inflammatory mediators as an adaptive response. The objective of the present study was to determine the consequences of HO-1 modulation in the murine collagen-induced arthritis (CIA) model. METHODS: DBA/1J mice were treated with an inhibitor of HO-1, tin protoporphyrin IX (SnPP), or with an inducer of HO-1, cobalt protoporphyrin IX (CoPP), from day 22 to day 29 after CIA induction. The clinical evolution of disease was monitored visually. At the end of the experiment, joints were examined for histopathologic changes. Cytokine levels in paws were measured by enzyme-linked…
Cytokine-mediated regulation of monocyte/macrophage cytotoxicity in human immunodeficiency virus-1 infection.
1992
Monocyte/macrophage-mediated tumor cytotoxicity was studied in patients infected with human immunodeficiency virus-1 (HIV-1) at various stages [Center for disease control (CDC) classification] of the disease. using the P-815 tumor cell line as target cells, the results demonstrated reduced monocyte/macrophage cytotoxicity early in HIV-1-related disease (CDCIII, P0.01). This cellular dysfunction sustained during the progression of the disease. Evidence could be presented that neither exogenous application of macrophage-stimulating cytokines (e.g. interferons) nor their endogenous induction in vitro restored monocyte/macrophage cytotoxicity. However, enhanced tumor necrosis factor (TNF)-alpha…
LAAE-14, a new anti-inflammatory drug, increases the survival ofCandida albicans-inoculated mice
2003
LAAE-14, a lipidic acid-amido ether derivative, has been recently described as a new anti-inflammatory drug. We have studied the effect of treatment with this compound on the susceptibility of mice to in vivo experimental Candida albicans infection. ICR mice orally treated with LAAE-14 (25 mg kg(-1)) and experimentally intravenously infected showed a significantly increased survival as compared to control mice. In vitro, the compound did not inhibit the growth of C. albicans yeast cells or the yeast-to-hyphal transition. The in vitro production of prostaglandin E2 by peritoneal macrophages in response to the yeasts and hyphae of C. albicans was significantly decreased upon treatment with LA…