Search results for "Acellular"

Defence Mechanisms and Stem Cells in Holothuria polii and Sipunculus nudus

2009

Holothuria polii (Phylum Echinodermata) and Sipunculus nudus (Phylum Sipuncula) have been used as model systems for studying invertebrate immunity. In the coelomic fluid of Holothuria polii, coelomocytes (i.e. amoebocytes, Type I, Type II, Type III spherule cells) and stem cells are present. After an injection of formalin-fixed sheep erythrocytes into the coelomic cavity, structural modifications in the animal’s, such as the coelomic cavity, stone canal and periesophageal ring were observed. Cellular events observed within the coelomic cavity include the recruitment of a large number of coelomocytes from the haemopoietic areas, phagocytosis carried out by amoebocytes and the formation of br…

PKSP-dependent reduction of phagolysosome fusion and intracellular kill of Aspergillus fumigatus conidia by human monocyte-derived macrophages.

2002

Summary Previously, we described the isolation of an Aspergillus fumigatus mutant producing non-pigmented conidia, as a result of a defective polyketide synthase gene, pksP (polyketide synthase involved in pigment biosynthesis). The virulence of the pksP mutant was attenuated in a murine animal infection model and its conidia showed enhanced susceptibility towards damage by monocytes in vitro. Because macrophage-mediated killing is critical for host resistance to aspergillosis, the interaction of both grey-green wild-type conidia and white pksP mutant conidia with human monocyte-derived macrophages (MDM) was studied with respect to intracellular processing of ingested conidia. After phagocy…

Resveratrol mediated cancer cell apoptosis, and modulation of multidrug resistance proteins and metabolic enzymes.

2019

Abstract Background The degree of intracellular drug accumulation by specific membrane transporters, i.e., MDR1, BCRP, and MRP, and the degree of detoxification by intracellular metabolic enzymes, i.e., CYP3A4 and GST, provide control for cancer chemotherapy through diminishing the propensity of cancer cells to undergo apoptosis which in turn modulates the unresolved and complex phenomenon of multidrug resistance (MDR) for the cancer cells. Hypothesis/Purpose This study dwells into the interaction details involving ABC-transporters, CYP3A4, GST and cytotoxic effects of resveratrol on different cell lines. Methods Resveratrol was evaluated for its ability modulating the expression and efflux…

γ δ T Cell Modulation in Anticancer Treatment

2010

The broad antimicrobial and antitumoral reactivity of Vgamma9Vdelta2 T cells, their ability to produce inflammatory cytokines involved in protective immunity against intracellular pathogens and tumors and their strong cytolytic and bactericidal activities suggest their direct involvement in immune control of cancers and infections. gammadelta T cells can be selectively activated by naturally occurring or synthetic phosphoantigens, and drugs that enhance their accumulation into stressed cells, offering new avenues for the development of gammadelta T cell-based immunotherapies. The recent development of small drugs selectively activating Vgamma9Vdelta2 T lymphocytes, which upregulate endogeno…

Variability of permeability estimation from different protocols of subculture and transport experiments in cell monolayers.

2014

Abstract Introduction In vitro models with high predictive ability have been revealed as strong tools for pharmaceutical industry. However, the variability in permeability estimations complicates the comparison and combination of data from different laboratories and it makes necessary the careful validation of the model and the continuous suitability demonstration. The adequate standardization of pre-experimental, experimental and post-experimental factors might help to reduce the inter- and intra-laboratory variability in permeability values. Methods The objective of this paper is the evaluation of the effect of passage number, experimental protocol, time after seeding and calculation meth…

Active contractility of the lymphangion and coordination of lymphangion chains

1976

Intracellul�re Verteilung von i.v. injiziertem Pb210 in Kaninchenorganen

1958

1. Die intracellulare Verteilung von Pb210wurde an Leber, Milz, Niere und Lunge von Kaninchen 1–35 Tage nach i.v. Applikation untersucht.

Über den Einfluss von Acetylcholin auf das Membranpotential denervierter Rattenzwerchfelle

1957

The membran potential of isolated rat-diaphragms has been measured by means of intracellular micro-electrodes, in order to study changes of the resting potential and of the depolarizing action of acetylcholine after section of the phrenic nerve. Within 80 days after denervation, the membrane potential was found to fall exponentially from 87 mV to 66 mV. The action of acetylcholine, on the other hand, was found to be independent of the duration of denervation: between the 4th and the 80th day of denervation: 10−5g/ml acetylcholine always caused the membrane potential to fall by an average of the 9 mV.

Isoform specificity of cardiac glycosides binding to human Na+,K+-ATPase α1β1, α2β1 and α3β1

2009

Abstract Cardiac glycosides inhibit the Na + ,K + -ATPase and are used for the treatment of symptomatic heart failure and atrial fibrillation. In human heart three isoforms of Na + ,K + -ATPase are expressed: α 1 β 1 , α 2 β 1 and α 3 β 1 . It is unknown, if clinically used cardiac glycosides differ in isoform specific affinities, and if the isoforms have specific subcellular localization in human cardiac myocytes. Human Na + ,K + -ATPase isoforms α 1 β 1 , α 2 β 1 and α 3 β 1 were expressed in yeast which has no endogenous Na + ,K + -ATPase. Isoform specific affinities of digoxin, digitoxin, β-acetyldigoxin, methyldigoxin and ouabain were assessed in [³H]-ouabain binding assays in the abse…

Isolation of endothelin A receptor from bovine lungs.

1995

We isolated endothelin receptor A (ET A ) from bovine lungs in a single-step purification procedure using antibodies raised against synthetic peptides that correspond to extra- and intracellular domains of the rat bradykinin receptor. Two receptor species of 55 and 35 kDa were isolated and subjected to N-terminal microsequencing. The difference between the observed and expected molecular weight species suggests that bovine ET A receptor is glycosylated.