Search results for "Acetoacetate"

showing 8 items of 8 documents

Metabolomics of Human Amniotic Fluid and Maternal Plasma during Normal Pregnancy

2016

Metabolic profiles of amniotic fluid and maternal blood are sources of valuable information about fetus development and can be potentially useful in diagnosis of pregnancy disorders. In this study, we applied 1H NMR-based metabolic profiling to track metabolic changes occurring in amniotic fluid (AF) and plasma (PL) of healthy mothers over the course of pregnancy. AF and PL samples were collected in the 2nd (T2) and 3rd (T3) trimester, prolonged pregnancy (PP) until time of delivery (TD). A multivariate data analysis of both biofluids reviled a metabolic switch-like transition between 2nd and 3rd trimester, which was followed by metabolic stabilization throughout the rest of pregnancy proba…

B Vitamins0301 basic medicineAmniotic fluidPhysiologyMaternal HealthPlacentalcsh:MedicineSpectrum analysis techniquesBiochemistryAcetoacetatesFetal DevelopmentPlasmachemistry.chemical_compoundGlucose MetabolismPregnancyPyruvic AcidBlood plasmaMedicine and Health SciencesMetabolitesAmino Acidslcsh:ScienceMultidisciplinary3-Hydroxybutyric AcidOrganic CompoundsObstetrics and GynecologyHematologyVitaminsKetonesBody FluidsChemistryBloodmedicine.anatomical_structurePregnancy Trimester SecondPhysical SciencesMetabolomeKetone bodiesCarbohydrate MetabolismFemaleAnatomyResearch Articlemedicine.drugPyruvateAdultmedicine.medical_specialtyPregnancy Trimester ThirdGestational AgeCholinesBiologyBlood PlasmaYoung Adult03 medical and health sciencesNMR spectroscopyInternal medicinePlacentamedicineHumansMetabolomicsCarnitineFetusPregnancy030102 biochemistry & molecular biologylcsh:ROrganic ChemistryChemical CompoundsBiology and Life SciencesAmniotic Fluidmedicine.diseaseResearch and analysis methodsMetabolismGlucose030104 developmental biologyEndocrinologychemistryWomen's Healthlcsh:QPyruvic acidAcidsPLOS ONE
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Hibernation impact on the catalytic activities of the mitochondrial D-3-hydroxybutyrate dehydrogenase in liver and brain tissues of jerboa (Jaculus o…

2003

Abstract Background Jerboa (Jaculus orientalis) is a deep hibernating rodent native to subdesert highlands. During hibernation, a high level of ketone bodies i.e. acetoacetate (AcAc) and D-3-hydroxybutyrate (BOH) are produced in liver, which are used in brain as energetic fuel. These compounds are bioconverted by mitochondrial D-3-hydroxybutyrate dehydrogenase (BDH) E.C. 1.1.1.30. Here we report, the function and the expression of BDH in terms of catalytic activities, kinetic parameters, levels of protein and mRNA in both tissues i.e brain and liver, in relation to the hibernating process. Results We found that: 1/ In euthemic jerboa the specific activity in liver is 2.4- and 6.4- fold high…

MaleBDH: D-3-hydroxybutyrate dehydrogenaseAcetoacetateBlotting Westernlcsh:Animal biochemistryBrainRodentiaBlotting NorthernCatalysisJerboa: <it>Jaculus orientalis</it>Mitochondrialcsh:BiochemistryHydroxybutyrate DehydrogenaseKineticsLiverHibernationJerboa: Jaculus orientalisKetone bodiesAnimalslcsh:QD415-436RNA Messengerlcsh:QP501-801Research ArticleBOH : D-3-hydroxybutyrateBMC Biochemistry
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Dynamics of ketone body metabolism in diabetic rats.

1972

Steady state blood levels of ketone bodies during infusions of acetoacetate at various rates have been compared in healthy and diabetic rats. The characteristics of the metabolic elimination of ketone bodies from the blood are completely changed in diabetic rats. Whereas steady state levels of ketone bodies increase linearly with the infusion rate in healthy rats, this increase is exponential in diabetic animals. This difference, which is due to an impaired metabolic elimination, becomes evident only above a dosage of 50 μmoles acetoacetate per kg per min. Chronic treatment with insulin for 4–6 days, but not acute insulin injection, restores the capacity of diabetic rats to metabolize keton…

Malemedicine.medical_specialtyDiabetic ketoacidosisHungerEndocrinology Diabetes and Metabolismmedicine.medical_treatmentRemission SpontaneousKetone BodiesStreptozocinAcetoacetatesDiabetes mellitusInternal medicineInternal MedicinemedicineDiabetes MellitusAnimalsInsulinAnalysis of VarianceChemistryInsulinmedicine.diseaseStreptozotocinKetoacidosisRatsEndocrinologyKetone bodiesSteady state (chemistry)KetosisAcidosismedicine.drugDiabetologia
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Action of metyrapone on the redox state of free nicotinamide-adenine dinucleotide and on oxygen consumption of perfused rat livers and isolated mitoc…

1971

Metyrapone [2-methyl-1,2-bis-(3-pyridyl)-1-propanone] in a concentration of 5 mM increased the lactate/pyruvate ratio and theΒ-hydroxybutyrate/ acetoacetate ratio in the perfusion fluid of the isolated rat liver by a factor of 6 indicating a considerable shift in the ratio of free [NAD]/[NADH] in both the cytoplasmic and the mitochondrial compartment. Oxygen uptake of the isolated liver was decreased to about one half. The onset of the inhibitory effect on the respiration of the isolated organ was immediate. The inhibition lasted for at least 1 h.

Malemedicine.medical_specialtychemistry.chemical_elementHydroxybutyratesMitochondria LiverNicotinamide adenine dinucleotideIn Vitro TechniquesOxygenRedoxAcetoacetatesElectron Transportchemistry.chemical_compoundHydroxybutyrate DehydrogenaseOxygen ConsumptionInternal medicineRespirationmedicineAnimalsPyruvatesPharmacologyMetyraponeChemistryGeneral MedicineCompartment (chemistry)MetyraponeNADRatsPerfusionKineticsEndocrinologyBiochemistryLiverCytoplasmSpectrophotometryDepression ChemicalLactatesNAD+ kinasemedicine.drugFerrocyanidesPolarographyNaunyn-Schmiedebergs Archiv fur Pharmakologie
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Regioselective substitution of 6,7-dichloroquinoline-5,8-dione: synthesis and X-ray crystal structure of 4a,10,11-triazabenzo[3,2-a]fluorene-5,6-dion…

2003

6,7-Dichloroquinoline-5,8-dione (1) was reacted with a number of 2-aminopyridine derivatives. Of the several possible products of this reaction, 4a,10,11-triazabenzo[3,2-a]fluorene-5,6-dione (6), produced by condensation and rearrangement, was obtained as the major product, and its structure was subsequently unambigously determined by X-ray crystallographic study. Ortho-quinones were produced via nucleophilic substitution at position C7, which was unexpected, considering that para-quinones were produced via C6 substitution in the reaction between compound 1 and ethyl acetoacetate in our previous work. Such unexpected nucleophilic substitution at C7 provides an effective, yet simple route, t…

StereochemistryClinical BiochemistryPharmaceutical ScienceAntineoplastic AgentsCrystallography X-RayBiochemistryMedicinal chemistryChemical synthesisInhibitory Concentration 50chemistry.chemical_compoundNucleophilic aromatic substitutionDrug DiscoveryTumor Cells CulturedNucleophilic substitutionHumansMolecular BiologySubstitution reactionFluorenesMolecular StructureOrganic ChemistryQuinonesRegioselectivityStereoisomerismQuinonechemistryDoxorubicinEthyl acetoacetateQuinolinesMolecular MedicineAcid hydrolysisDrug Screening Assays AntitumorBioorganic &amp; Medicinal Chemistry
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Prodrugs of sulfacetamide: Synthesis, X-ray structure, Hirshfeld analysis, antibacterial assessment, and docking studies

2022

Abstract New prodrugs of sulfacetamide as azo compounds were synthesized and have been evidenced through elemental and spectral analyses. Their synthesis was carried out by coupling the diazonium salt of sulfacetamide with activated carbanion salt of ethyl acetoacetate at 0 ˚C to afford the hydrazono derivative 3. Other prodrugs as sulfacetamide-pyrazoles, 5a, 5b and 5c were furnished via cyclocondensation of 3 with aryl/heteroaryl hydrazines. X-ray diffraction for single crystal was used to confirm the molecular and supramolecular structures of 3. In addition, DFT studies were performed to analyze the geometric parameters and compute the electronic properties of 3 and 5a-c. Hirshfeld analy…

biologyArylOrganic ChemistrySupramolecular chemistryActive siteSulfacetamideProdrugCombinatorial chemistryAnalytical ChemistryInorganic Chemistrychemistry.chemical_compoundchemistryDocking (molecular)Ethyl acetoacetatebiology.proteinmedicineSpectroscopyCarbanionmedicine.drugJournal of Molecular Structure
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9-Ethoxy-1,5,13-trimethyl-8,10-dioxatetracyclo[7.7.1.02,7.011,16]heptadeca-2,4,6,11,13,15-hexaene

2009

The reaction of ethyl acetoacetate with meta-cresol in an acidic ionic liquid yielded a complex mixture of condensation products. 4,7-Dimethylcoumarin and the title compound, C20H22O3, were isolated. The title compound shows chemical but not crystallographic mirror symmetry. The two aromatic rings are inclined at an angle of 73.55&amp;#8197;(6)&amp;#176;.

biologyCondensationAromaticityGeneral ChemistryCondensed Matter PhysicsHEXAbiology.organism_classificationBioinformaticsMedicinal chemistrylcsh:Chemistrychemistry.chemical_compoundchemistrylcsh:QD1-999Ethyl acetoacetateIonic liquidTetraGeneral Materials ScienceDeca-Ene reactionActa Crystallographica Section E
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H2-Antihistaminika, 33. Mitt. Synthese und H2-antagonistische Aktivität heteroaromatischer (Thio)Carboxamide und Triazol(thi)on-Derivate des Piperidi…

1987

Die Zyklisierung der (Thio)Semicarbazide la, b mit N-Cyan-diphenylimidocarbonat, N-Cyan-dimethyldithioimidocarbonat, Aminocrotonsaurenitril und Acetessigester gibt die heteroaromatischen (Thio)Carboxamide 5a, b und 7–9. Beim Einwirken von NaOH auf die (Thio)Biharnstoffe 12 und 13 sowie die (Oxa)Thiadiazoldiamine 19 und 20 werden die Triazol(thi)one 15 und 17 erhalten. Am Vorhof des Meerschweinchens zeigen 5a, 7 und 8 eine mit Cimetidin vergleichbare Histamin-H2-antagonistische Wirkung. H2-Antihistaminics, XXXIII: Synthesis and H2-Antagonistic Activity of Heteroaromatic (Thio)Carboxamides and Triazole(thi)one-Derivatives of Piperidinomethylphenoxypropylamine The (thio)semicarbazides la, b ar…

chemistry.chemical_classificationSemicarbazideSemicarbazidesStereochemistrymedicine.drug_classTriazolePharmaceutical ScienceThio-Carboxamidechemistry.chemical_compoundchemistryEthyl acetoacetateDrug DiscoverymedicineAliphatic compoundThioamideArchiv der Pharmazie
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