Search results for "Aglycone"

showing 10 items of 17 documents

Acceptor Specificity of Amylosucrase from Deinococcus radiopugnans and Its Application for Synthesis of Rutin Derivatives

2016

The transglycosylation activity of amylosucrase (ASase) has received significant attention owing to its use of an inexpensive donor, sucrose, and broad acceptor specificity, including glycone and aglycone compounds. The transglycosylation reaction of recombinant ASase from Deinococcus radiopugnans (DRpAS) was investigated using various phenolic compounds, and quercetin-3-O-rutinoside (rutin) was found to be the most suitable acceptor molecule used by DRpAS. Two amino acid residues in DRpAS variants (DRpAS Q299K and DRpAS Q299R), assumed to be involved in acceptor binding, were constructed by site-directed mutagenesis. Intriguingly, DRpAS Q299K and DRpAS Q299R produced 10-fold and 4-fold hig…

0106 biological sciences0301 basic medicineGlycosylationGlycosylationStereochemistryRutinAmino Acid Motifs01 natural sciencesApplied Microbiology and BiotechnologySubstrate Specificity03 medical and health sciencesRutinchemistry.chemical_compoundAmylosucraseGlucosyltransferasesBacterial Proteins010608 biotechnologyDeinococcusBinding siteBinding SitesbiologyGeneral Medicinebiology.organism_classificationAcceptorMolecular Docking SimulationKinetics030104 developmental biologyAglyconechemistryGlucosyltransferasesbiology.proteinDeinococcusBiotechnologyJournal of Microbiology and Biotechnology
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Chromatographic separation and partial identification of glycosidically bound volatile components of fruit

1990

Abstract Synthetic monoterpenic and aromatic β- d -glucosides and β- d -rutinosides were separated by Fractogel TSK HW-40 S chromatography according to their molecular size and interactions occurring between the aglycone moiety and the gel matrix. Under these conditions terpenyl rutinosides were eluted before the homologous glucoside derivatives. In the two classes, monoglucosides and rutinosides, aromatic glycosidically bound components had lower retention times than the corresponding terpenyl components. The use of over-pressure layer chromatography (OPLC) allowed the separation of glucoside and rutinoside derivatives, and in these two classes aromatic and several monoterpene compounds we…

0106 biological sciencesChromatography[CHIM.ANAL] Chemical Sciences/Analytical chemistryElutionSilica gel010401 analytical chemistryOrganic ChemistryGeneral MedicineFractionation01 natural sciencesBiochemistryHigh-performance liquid chromatographyThin-layer chromatography0104 chemical sciencesAnalytical ChemistryGel permeation chromatographychemistry.chemical_compoundAglyconechemistry[CHIM.ANAL]Chemical Sciences/Analytical chemistryOrganic chemistryGas chromatographyComputingMilieux_MISCELLANEOUS010606 plant biology & botany
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Potential Uses of Olive Oil Secoiridoids for the Prevention and Treatment of Cancer: A Narrative Review of Preclinical Studies

2021

The Mediterranean diet (MD) is a combination of foods mainly rich in antioxidants and anti-inflammatory nutrients that have been shown to have many health-enhancing effects. Extra-virgin olive oil (EVOO) is an important component of the MD. The importance of EVOO can be attributed to phenolic compounds, represented by phenolic alcohols, hydroxytyrosol, and tyrosol, and to secoiridoids, which include oleocanthal, oleacein, oleuropein, and ligstroside (along with the aglycone and glycosidic derivatives of the latter two). Each secoiridoid has been studied and characterized, and their effects on human health have been documented by several studies. Secoiridoids have antioxidant, anti-inflammat…

0301 basic medicineSettore MED/09 - Medicina InternaSettore CHIM/10 - Chimica Degli AlimentiMediterranean dietAnti-Inflammatory AgentsReviewDiet MediterraneanAntioxidantsCyclopentane Monoterpeneslcsh:Chemistrychemistry.chemical_compound0302 clinical medicineGlucosidesNeoplasmsIridoidslcsh:QH301-705.5SpectroscopyTraditional medicineGeneral MedicinePhenylethyl AlcoholComputer Science Applications030220 oncology & carcinogenesissecoiridoidsIridoid GlucosidesAntineoplastic AgentsoleocanthalCatalysisInorganic Chemistry03 medical and health sciencesPhenolsOleuropeinOleocanthalmedicineAnimalsHumanscanceroleaceinPhysical and Theoretical ChemistryOlive OilMolecular BiologyPyransAldehydesOrganic ChemistryCancermedicine.diseaseTyrosol030104 developmental biologyAglyconelcsh:Biology (General)lcsh:QD1-999chemistryoleuropeinligstrosideHydroxytyrosolOlive oilInternational Journal of Molecular Sciences
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Enriched cereal bars are more effective in increasing plasma quercetin compared with quercetin from powder-filled hard capsules

2011

The flavonol quercetin, is one of the major flavonoids found in edible plants. The bioavailability of quercetin in humans may be influenced by the food matrix in which it is consumed as well as by its chemical and physical form. The objective of the present study was to investigate the biokinetics of quercetin from quercetin-enriched cereal bars and quercetin powder-filled hard capsules. In a randomised, single-blinded, diet-controlled cross-over study, six healthy women aged 22–28 years took a single oral dose of approximately 130 mg quercetin equivalents from either quercetin-enriched cereal bars (containing 93·3 % quercetin aglycone plus 6·7 % quercetin-4′-glucoside) or quercetin powder-…

AdultMedicine (miscellaneous)CapsulesPilot ProjectsDisaccharidesHigh-performance liquid chromatographyAntioxidantsSingle oral doseYoung Adultchemistry.chemical_compoundHumansIngestionSingle-Blind MethodFood scienceAntihypertensive AgentsIsorhamnetinCross-Over StudiesNutrition and DieteticsBioavailabilityKineticsTamarixetinAglyconechemistryBiochemistryDietary SupplementsFood FortifiedFast FoodsFemaleQuercetinPowdersEdible GrainQuercetinNutritive ValueBritish Journal of Nutrition
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Betacyanins as phenol antioxidants. Chemistry and mechanistic aspects of the lipoperoxyl radical-scavenging activity in solution and liposomes.

2009

Reaction kinetics of betanin and its aglycone betanidin towards peroxyl radicals generated from the azo-initiated oxidation of methyl linoleate in methanol, and of a heterogeneous aqueous/soybean phosphatidylcholine liposomal system, were studied by monitoring formation of linoleic acid hydroperoxides and consumption of the pigments. Betanin was a weak retarder in methanol, and an effective chain breaking antioxidant in the liposomal model, indicating that kinetic solvent effects and partition in lipid bilayers may affect its activity. Betanidin behaved as a chain terminating antioxidant in both models. Kinetic parameters characterizing peroxyl radical-scavenging activity showed that betani…

Antioxidantmedicine.medical_treatmentLipid Bilayersalpha-TocopherolBiochemistryChemical kineticsLinoleic Acidchemistry.chemical_compoundStructure-Activity RelationshipReaction rate constantSettore BIO/10 - BiochimicamedicineBetacyaninsOrganic chemistryChromatography High Pressure LiquidBetaninAqueous solutionMolecular StructureMethanolWaterDrug SynergismGeneral MedicineFree Radical ScavengersSolutionsAglyconechemistryLinoleic Acidsbetacyanins betanin betanidin lipid peroxides liposomes antioxidant phytochemicalsSpectrophotometryLiposomesPhosphatidylcholinesSolventsMethanolBetacyaninsLipid PeroxidationOxidation-ReductionFree radical research
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Solar UV-B radiation affects leaf quality and insect herbivory in the southern beech tree Nothofagus antarctica

2004

We examined the effects of solar ultraviolet-B (UV-B) radiation on plant-insect interactions in Tierra del Fuego (55°S), Argentina, an area strongly affected by ozone depletion because of its proximity to Antarctica. Solar UV-B under Nothofagus antarctica branches was manipulated using a polyester plastic film to attenuate UV-B (uvb-) and an Aclar film to provide near-ambient UV-B (uvb+). The plastic films were placed on both north-facing (i.e., high solar radiation in the Southern Hemisphere) and south-facing branches. Insects consumed 40% less leaf area from north- than from south-facing branches, and at least 30% less area from uvb+ branches than from uvb-branches. The reduced herbivory …

EcophysiologyInsectaUltraviolet RaysPlastic filmAntarctic RegionsTreesGALLIC ACIDCiencias Biológicaschemistry.chemical_compoundBotanyAnimalsOZONE DEPLETIONBeechEcosystemEcology Evolution Behavior and SystematicsNothofagusbiologyFLAVONOIDSbiology.organism_classificationOzone depletionFagaceaePlant LeavesAglyconechemistryLarvaSunlightNothofagus antarcticaOtros Tópicos BiológicosHERBIVORYNOTHOFAGUSCIENCIAS NATURALES Y EXACTAS
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Two new triterpenoid saponins fromPittosporum senaciaPutterlick (Pittosporaceae)

2012

From the branches of Pittosporum senacia Putterlick (Pittosporaceae), two new triterpenoid saponins, senaciapittosides A and B (1, 2), were isolated. Their structures were elucidated by extensive analysis of one- and two-dimensional nuclear magnetic resonance spectroscopy, high-resolution electrospray ionization mass spectrometry (HR-ESIMS) and chemical evidence as 3-O-[β-d-glucopyranosyl-(1  2)]-[α-l-arabinopyranosyl-(1  3)]-[α-l-arabinofuranosyl-(1  4)]-β-d-glucuronopyranosyl oleanolic acid 28-O-β-d-glucopyranosyl ester (1) and 3-O-[β-d-glucopyranosyl-(1  2)]-[α-l-arabinopyranosyl-(1  3)]-[α-l-arabinofuranosyl-(1  4)]-β-d-glucuronopyranosyl-22-O-α-l-arabinopyranosyl-21-acetoxy R1-barrigen…

GlycosylationbiologyStereochemistryElectrospray ionizationPittosporaceaeGeneral ChemistryNuclear magnetic resonance spectroscopybiology.organism_classificationchemistry.chemical_compoundAglyconechemistryGeneral Materials SciencePittosporum senaciaOleanolic acidTwo-dimensional nuclear magnetic resonance spectroscopyMagnetic Resonance in Chemistry
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New Acylated Preatroxigenin Saponins from Atroxima congolana

2003

Eight new acylated preatroxigenin saponins 1–8 were isolated as four inseparable mixtures of the trans- and cis-4-methoxycinnamoyl derivatives, atroximasaponins A1/A2 (1/2), B1/B2 (3/4), C1/C2 (5/6) and D1/D2 (7/8) from the roots of Atroxima congolana. These compounds are the first examples of triterpene saponins containing preatroxigenin (=(2β,3β,4α,22β)-2,3,22,27-tetrahydroxyolean-12-ene-23,28-dioic acid as aglycone. Their structures were elucidated on the basis of extensive 1D- and 2D-NMR studies and FAB-MS as 3-O(β-D-glucopyranosyl)preatroxigenin 28-{O-β-D-xylopyranosyl-(14)-O-α-L-rhamnopyranosyl-(12)-O-[O-β-D-glucopyranosyl-(13)-β-D-glucopyranosyl-(13)]-4-O-(trans-4-methoxycinnamoyl)-β…

Inorganic Chemistrychemistry.chemical_classificationchemistry.chemical_compoundAglyconeTriterpeneChemistryStereochemistryOrganic ChemistryDrug DiscoveryPhysical and Theoretical ChemistryBiochemistryCatalysisHelvetica Chimica Acta
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Selectivity of Neutrophil 5-Lipoxygenase and Cyclo-oxygenase Inhibition by an Anti-inflammatory Flavonoid Glycoside and Related Aglycone Flavonoids

1988

Abstract A newly described plant-derived flavonoid, hypolaetin-8-glucoside, which has anti-inflammatory and gastroprotective actions in-vivo, and its corresponding aglycone, hypolaetin, have been compared with 14 other flavonoids for inhibition of eicosanoid generation via the 5-lipoxygenase and cyclo-oxygenase pathways in elicited rat peritoneal leukocytes stimulated with calcium ionophore. Comparable results for the inhibitory profiles of the compounds were obtained using either radioimmunoassay of released eicosanoids or radio-TLC of metabolites formed from labelled arachidonate, but there were differences in absolute potency of the inhibitors. Hypolaetin-8-glucoside was a weak but selec…

MaleNeutrophilsStereochemistryFlavonoidRadioimmunoassayPharmaceutical ScienceArachidonic AcidsIn Vitro TechniquesArachidonate LipoxygenasesLipoxygenasechemistry.chemical_compoundEicosanoic AcidsAnimalsCyclooxygenase InhibitorsLipoxygenase InhibitorsIC50CalcimycinFlavonoidsPharmacologychemistry.chemical_classificationArachidonic AcidbiologyAnti-Inflammatory Agents Non-SteroidalGlycosideRats Inbred StrainsBiological activityRatsAglyconeBiochemistrychemistryEnzyme inhibitorbiology.proteinHypolaetinChromatography Thin LayerJournal of Pharmacy and Pharmacology
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Hederagenin glycosides from the fruits of Blighia unijugata

2019

Abstract A phytochemical investigation of Blighia unijugata led to the isolation of eleven hederagenin glycosides. Among these compounds, six are previously undescribed, two are described in their native forms for the first time and three are known whereas firstly isolated from Blighia unijugata. The structure of the undescribed compounds was elucidated on the basis of 2D NMR and mass spectrometry analyses as 3-O-β-D-xylopyranosyl-(1 → 3)-α-L-arabinopyranosyl-(1 → 4)-β-D-glucopyranosyl-(1 → 3)-α-L-rhamnopyranosyl-(1 → 2)-α-L-arabinopyranosylhederagenin, 3-O-β-D-xylopyranosyl-(1 → 3)-α-L-arabinopyranosyl-(1 → 4)-3-O-acetyl-β-D-glucopyranosyl-(1 → 3)-α-L-rhamnopyranosyl-(1 → 2)-α-L-arabinopyr…

Models Molecular0106 biological sciencesStereochemistryMolecular ConformationPlant ScienceHorticulture01 natural sciencesBiochemistrychemistry.chemical_compoundGlycosidesOleanolic AcidBlighia unijugataMolecular Biologychemistry.chemical_classificationbiology010405 organic chemistryGlycosideGlycosidic bondGeneral MedicineBlighiabiology.organism_classification0104 chemical sciencesHederageninAglyconechemistryPhytochemicalFruitTwo-dimensional nuclear magnetic resonance spectroscopyBlighia010606 plant biology & botanyPhytochemistry
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