Search results for "Allergy"
showing 10 items of 3181 documents
Relationship between the target antigen of liver-kidney microsomal (LKM) autoantibodies and rat isoenzymes of cytochrome P-450
1988
Chronic active hepatitis (CAH) is a clinical syndrome of different etiologies. Liver-kidney microsomal (LKM) autoantibodies characterize a subgroup of HBsAg negative CAH, which is considered to be an autoimmune liver disease. By immunoblotting analysis (IB) LKM positive sera have been shown to react strongly with a poly-peptide band at 50 kD. Therefore we investigated various rat microsomal enzymes with a molecular weight around 50 kD as potential candidate target antigens. These included epoxide hydrolase, cytochrome P-450 reductase, and phenobarbital-inducible isoenzymes of cytochrome P-450 (PB1, PB2, PB3a, PB3b). By radioimmunoassay (RIA) and IB LKM positive sera were shown to react with…
Generation of immune responses against hepatitis C virus by dendritic cells containing NS5 protein-coated microparticles.
2009
ABSTRACTDendritic cells (DCs) internalize and process antigens as well as activate cellular immune responses. The aim of this study was to determine the capacity of DCs that contain antigen-coated magnetic beads to induce immunity against the nonstructural hepatitis C virus (HCV) antigen 5 (NS5). Splenocytes derived from Fms-like tyrosine kinase receptor 3 (Flt3) ligand-pretreated BALB/c mice were incubated with magnetic beads coated with HCV NS5, lipopolysaccharide (LPS), and/or anti-CD40; purified; and used for immunization. Cellular immunity was measured using cytotoxic T-lymphocyte (CTL) and T-cell proliferation assays, intracellular cytokine staining, and a syngeneic tumor challenge us…
Cloning of Clostridium difficile toxin B gene and demonstration of high N-terminal homology between toxin A and B.
1990
High titered Clostridium sordellii lethal toxin antiserum, cross-reactive with C. difficile cytotoxin B (ToxB), was used to isolate toxB fragments from a C. difficile expression library. Recombinant clones containing toxB fragments of the 5' and 3' end were isolate. A 2.5-kb HincII fragment of chromosomal DNA overlaps both groups of clones. A partial restriction map of the total toxB gene is presented. The gene is positioned upstream of utxA and toxA, toxB has a size of 6.9 kb, corresponding to a 250-kDa polypeptide. A partial sequence of the 5' end of toxB was determined. The sequence contains 398 bp upstream of toxB with a putative Shine-Dalgarno box (AGGAGA) and 609 bp of the toxB open r…
Minimum information about a biofilm experiment (MIABiE): standards for reporting experiments and data on sessile microbial communities living at inte…
2014
The minimum information about a biofilm experiment (MIABiE) initiative has arisen from the need to find an adequate and scientifically sound way to control the quality of the documentation accompanying the public deposition of biofilm-related data, particularly those obtained using high-throughput devices and techniques. Thereby, the MIABiE consortium has initiated the identification and organization of a set of modules containing the minimum information that needs to be reported to guarantee the interpretability and independent verification of experimental results and their integration with knowledge coming from other fields. MIABiE does not intend to propose specific standards on how biof…
Ku teologicznemu pojęciu religii
2018
The concept of religion is a fundamental concept of religiological sciences. Still it is not devoid of difficulties. Its critics attempt to prove that plurality and variety of religious phenomena excludes the possibility to define religion. On the other hand the lack of the general concept of religion constitutes a serious problem for theology of religion since it is difficult to study relationships between Christianity and other religions not knowing what religion is and what its theological essence is. The present article consists of three parts. The first part analyses the history of the concept of religion (Latin religio). Second part considers the problem of the definition of religion.…
Psychologiczna koncepcja poszukiwania sacrum w religijności i duchowości
2012
Nowadays, there is a growing interest in the area of psychology of religion as regards religiousness and spirituality. In the past both terms overlapped substantially which was clearly visible in great spiritual traditions of many religions. Recently, however, spirituality has emerged as a distinct construct. Although, religiousness and spirituality have common characteristics they are generally regarded as related but not identical constructs. Both religiousness and spirituality are embedded within the context of “a search for the sacred” which refers to sacrum. The main difference is that religiousness represents the personal or communal search for the sacred that happens within a traditi…
Práctica Española en materia de Tratados Internacionales correspondiente al año 1994
2018
I.- TRAMITACIÓN PARLAMENTARIA DE LOS TRATADOS EN ESPAÑA DURANTE 1994. 1. Tratados para los que se solicita la autorización previa de las Cortes Generales (art.s.93 y 94-1 CE). 2. Tratados de los que se da información posterior a las Cortes Generales (art 94-2 CE). 3. Tramitación parlamentaria de la denuncia de los tratados (art. 96-2 CE). 4. Corrección de errores. II.- PUBLICIDAD DE LOS TRATADOS EN ESPAÑA. 1. Tratados publicadas en el Boletín Oficial del Estado durante 1994: A) Tratados multilaterales; B) Tratados bilaterales. 2. Enmiendas o modificaciones a los tratados publicadas en 1994. 3. Tratados cuya entrada en vigor se comunica en 1994. 4. Corrección de errores relativas a textos de…
La protección internacional de zonas y especies en la región mediterránea
2018
INTRODUCCIÓN.- PARTE PRIMERA: ZONAS Y ESPECIES PROTEGIDAS INTERNACIONALMENTE EN LA REGION MEDITERRÁNEA. I. LAS ZONAS ESPECIALMENTE PROTEGIDAS Y LA PROTECCIÓN DE LAS ESPECIES EN EL DERECHO INTERNACIONAL. 1. El planteamiento aislante. 2. El planteamiento comprehensivo. a) Los tratados pioneros. b) De la Declaración de Estocolmo en adelante. 3. Hacia la utilización sostenible. II. LAS ZONAS ESPECIALMENTE PROTEGIDAS y LA PROTECCIÓN DE LAS ESPECIES EN EL MEDITERRÁNEO. 1. El antiguo marco para la protección del Mar Mediterráneo contra la contaminación. 2. El período de transición. 3. El nuevo marco para la protección del medio marino y de la región costera del Mediterráneo. PARTE SEGUNDA: LA REGL…
Práctica Española en materia de Tratados Internacionales correspondiente al año 1996
2018
El presente trabajo analiza cuatro aspectos correspondientes a la práctica española sobre celebración de tratados internacionales durante el año 1996. Estos aspectos son: a) la tramitación parlamentaria de los tratados internacionales; b) la publicidad de los tratados; c) las alteraciones en el listado de Estados partes en los tratados internacionales en los que España es Estado parte; y d) una selección de las declaraciones oficiales relativas a tratados en los que España es Estado parte
Práctica Española en materia de Tratados Internacionales correspondiente al año 1997
2018
El presente trabajo analiza cuatro aspectos correspondientes a la práctica española sobre celebración de tratados internacionales durante el año 1997. Estos aspectos son: a) la tramitación parlamentaria de los tratados internacionales; b) la publicidad de los tratados; c) las alteraciones en el listado de Estados partes en los tratados internacionales en los que España es Estado parte; y d) una selección de las declaraciones oficiales relativas a tratados en los que España es Estado parte