Search results for "Amide"
showing 10 items of 3119 documents
Mit Donorzentren versehener korbförmiger Molekülhohlraum — Darstellung, Struktur, Eigenschaften
1991
Basket-shaped Molecular Cavity-Containing Donor Centres — Synthesis, Structure, Properties The macrocyclic basket-shaped molecule 2, composed of three 4-donor-substituted pyridine units, is synthesized by cyclisation of the chloromethyl compound 7 with sulfonamide 8. The X-ray structure analysis of 2 gives an impression of the shape of the molecular basket. Furthermore it demonstrates that the toluenesulfonamide residues interlink, creating dimeric units of 2 in the crystal.
Amino Acid derivatives as new zinc binding groups for the design of selective matrix metalloproteinase inhibitors.
2012
A number of matrix metalloproteinases (MMPs) are important medicinal targets for conditions ranging from rheumatoid arthritis to cardiomyopathy, periodontal disease, liver cirrhosis, multiple sclerosis, and cancer invasion and metastasis, where they showed to have a dual role, inhibiting or promoting important processes involved in the pathology. MMPs contain a zinc (II) ion in the protein active site. Small-molecule inhibitors of these metalloproteins are designed to bind directly to the active site metal ions. In an effort to devise new approaches to selective inhibitors, in this paper, we describe the synthesis and preliminary biological evaluation of amino acid derivatives as new zinc b…
Comparison of the interaction of cobalt bovine carbonic anhydrase II with acetazolamide and methazolamide and the reaction of apoenzyme with cobalt(I…
2003
The metalloenzyme carbonic anhydrase (CA) is an attractive choice for a research-based bioinorganic laboratory course. In this project the interaction of cobalt bovine carbonic anhydrase II (CoBCAII) with acetazolamide and methazolamide and the reaction of apoenzyme with cobalt(II) complexes of acetazolamide and methazolamide is studied by UV-visible spectroscopy. Prior to this spectroscopic study students are given native BCAII, and they prepare apoBCAII and CoBCAII. A major aim is to provide experience in handling metalloproteins and in the study of metal complexes-protein interactions.
Expression of silicatein in spicules from the Baikalian sponge
2005
Lake Baikal harbors the largest diversity of sponge species [phylum Porifera] among all freshwater biotopes. The abundantly occurring species Lubomirskia baicalensis was used to study the seasonal silicatein metabolism; the spicules of this species have an unusually thick axial filament, consisting of silicatein, which remains constant in diameter during their growth. In the course of maturation, the size of the silicic acid shell grows, until the final diameter of the spicules of about 8 microm is reached. The seasonal content of silicatein was assessed by use of antibodies raised against silicatein; they stained specifically the axial filaments. In addition we determined, by application o…
Biochemical Characterization of an Acetylcholine-hydrolyzing Enzyme from Bean Seedlings.
1980
An acetylcholine hydrolyzing enzyme was prepared and purified (40 times) from dwarf bean hypocotyl hooks. The purity of the enzyme was proved by polyacrylamide gel electrophoresis. The molecular weight of the enzyme was determined to be 65,000 daltons. Enzyme activity was the highest at pH 8.0 and between 30 and 36 C. The enzyme had an apparent affinity constant (K(m)) for acetylcholine of 460/micromolar. The affinity for substrate analogs increased from butyrylthiocholine to propionylthiocholine to acetylthiocholine. The enzyme activity was inhibited by choline, neostigmine, physostigmine, manganese, and calcium. Magnesium had no influence on the enzyme activity. We conclude that the enzym…
Reducing of acrylamide formation in wheat biscuits supplemented with flaxseed and lupine
2016
Abstract The use of pseudo-cereals for wheat products making is to fortify the deficiency of nutritional value in wheat flour. However rich in proteins plant additives could increase acrylamide content in baked products. The present study was focused on acrylamide reduction in wheat flour biscuits supplemented with lupine and defatted flaxseed flour treated by solid state (SSF) and submerged (SMF) fermentations by Lactobacillus sakei , Pediococcus pentosaceus and Pediococcus acidilactici strains. After fermentation the decrease in asparagine was on average of 67.6 and 80.6%, and reducing sugar contents were reduced by 18 and 79.4% in flaxseed and lupine, respectively. The most effective acr…
Structural and functional models for the dinuclear copper active site in catechol oxidases
2003
Two new mu-methoxo-bridged dinuclear copper(II) complexes with a N-substituted sulfonamide, [Cu(mu-OMe)(L)(NH(3))](2) (1) and [Cu(mu-OMe)(L)(DMSO)](2) (2) [HL, N-2-(4-methylbenzothiazole)benzenesulfonamide], have been prepared and characterized by single-crystal X-ray difraction analyses. Compound 1 crystallizes in the monoclinic space group C(2)/c with a=22.0678(18), b=7.9134(7), c=21.1186(18)A, beta=113.788(4) degrees and Z=8. Compound 2 crystallizes in the monoclinic space group C(2)/c with a=18.0900(10), b=9.5720(10), c=24.2620(10) A, beta=98.7120(10) degrees and Z=8. In both complexes the copper atoms have square-planar environments bridged by two oxygen atoms from methoxide groups. Ma…
FT-IR spectroscopic investigation of bacterial cell envelopes from Zymomonas mobilis which have different surface hydrophobicities
2013
Abstract Changes in the cell envelope composition of bacteria Zymomonas mobilis 113S which resulted in varied cell surface hydrophobicity (CSH) were examined using FT-IR-spectroscopy and conventional methods of biochemical analysis. Significant differences in all analyses were detected between hydrophilic and hydrophobic compounds in the envelopes of Z. mobilis . A significant positive linear relationship ( P Z. mobilis and the ratio of absorbance band intensities assigned to the CH 2 and CH 3 vibrations from lipids as well as the asymmetric and symmetric stretching vibrations of these methylene and methyl groups. Differences were also seen between the ratio of band absorbances arising from…
Discelectrophoretischer Nachweis der Heterogenität der menschlichen sauren Spermaphosphatase
2009
Zusammenfassung Mit Hilfe der vertikalen Discelektrophorese in Polyacrylamid-Gel ist es moglich, zwei Enzymbanden der sauren Spermaphosphatase nachzuweisen. Eine optimale Trennung ergibt sich bei einer Wasserstoffionenkonzentration von pH 7,5 im Trenn-Gel und pH 5,5 im Sammel-Gel und 0,1 M Acetatpuffer. Der Vorteil dieser Methode liegt in dem relativ geringen apparativen Aufwand, der kurzen Trennzeit, der hohen Trennscharfe sowie der Moglichkeit, aserst geringe Substanzmengen aufzutrennen. Summary With the aid of disc-electrophoresis in poly acryl amide it is possible to proof the heterogeneity of acid phosphatase of human seminal fluid. In all samples could be detected. The best results we…
Study on the reduction of acrylamide in mixed rye bread by fermentation with bacteriocin-like inhibitory substances producing lactic acid bacteria in…
2013
Abstract This study mainly focuses on a strategy for reducing of acrylamide in cereal products, predominantly in bakery products. The effect of mesophilic lactic acid bacteria (LAB) strains as well as a novel fermentation media on the bases of extruded rye wholemeal on the acrylamide formation in mixed rye bread of different weight of loaf was studied. The LC–MS/MS method for acrylamide determination in bread crumb has been applied. Addition of 15% low pH (pH 3.4–4.3) fermented product to bread dough, which were produced by using commercial strain Lactobacillus casei and bacteriocin-like inhibitory substances (BLIS) producing strains Lactobacillus sakei KTU05-6 , Pediococcus acidilactici KT…