Search results for "Analysis method"

showing 10 items of 713 documents

Evidence for pleural epithelial-mesenchymal transition in murine compensatory lung growth

2017

In many mammals, including rodents and humans, removal of one lung results in the compensatory growth of the remaining lung; however, the mechanism of compensatory lung growth is unknown. Here, we investigated the changes in morphology and phenotype of pleural cells after pneumonectomy. Between days 1 and 3 after pneumonectomy, cells expressing α-smooth muscle actin (SMA), a cytoplasmic marker of myofibroblasts, were significantly increased in the pleura compared to surgical controls (p < .01). Scanning electron microscopy of the pleural surface 3 days post-pneumonectomy demonstrated regions of the pleura with morphologic features consistent with epithelial-mesenchymal transition (EMT); nam…

MaleB VitaminsThin-Layer Chromatography0301 basic medicinePathologyOrganogenesismedicine.medical_treatmentVimentinBiochemistryMiceSpectrum Analysis Techniques0302 clinical medicineMedicine and Health SciencesElectron MicroscopyRespiratory System ProceduresPneumonectomyLungImage CytometryMicroscopyMultidisciplinarybiologyOrganic CompoundsChromatographic TechniquesQRVitaminsrespiratory systemChemistryPhenotypemedicine.anatomical_structureThoracotomySpectrophotometry030220 oncology & carcinogenesisPhysical SciencesPleuraMedicineScanning Electron MicroscopyMyofibroblastResearch Articlemedicine.medical_specialtyEpithelial-Mesenchymal TransitionImaging TechniquesScienceCompensatory growth (organ)BiotinSurgical and Invasive Medical ProceduresResearch and Analysis Methods03 medical and health sciencesPneumonectomyFluorescence ImagingmedicineAnimalsVimentinEpithelial–mesenchymal transitionLungSurgical ExcisionFluorimetryOrganic ChemistryChemical CompoundsBiology and Life SciencesProteinsrespiratory tract diseasesMice Inbred C57BLCytoskeletal ProteinsPlanar ChromatographyB vitamins030104 developmental biologybiology.proteinMesothelial CellPLOS ONE
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Acute Gastroenteritis and Campylobacteriosis in Swiss primary care: the viewpoint of general practitioners

2016

Acute gastroenteritis (AG) is frequently caused by infectious intestinal diseases (IID) including food- and waterborne pathogens of public health importance. Among these pathogens, Campylobacter spp. plays a major role. Many European countries monitor selected IIDs within disease surveillance systems. In Switzerland, the information on IIDs is restricted to limited surveillance data, while no data is available for AG. We conducted a qualitative study among Swiss general practitioners (GPs) to investigate the case management of AG and campylobacteriosis patients, the associated disease burden and the determinants leading to registration in the National Notification System for Infectious Dise…

MaleBacterial Diseases0301 basic medicinelcsh:MedicineNotification systemPathology and Laboratory MedicineFeces0302 clinical medicineCampylobacteriosisAntibioticsMedical adviceCampylobacter InfectionsMedicine and Health SciencesPublic and Occupational Health030212 general & internal medicinelcsh:ScienceDisease surveillanceMultidisciplinaryAntimicrobialsDrugsGastroenteritisBacterial PathogensInfectious DiseasesMedical MicrobiologyPathogensSwitzerlandResearch ArticleAdultDiarrheamedicine.medical_specialty030106 microbiologyMEDLINECampylobacteriosisGastroenterology and HepatologyResearch and Analysis MethodsMicrobiology03 medical and health sciencesSigns and SymptomsGeneral PractitionersDiagnostic MedicineMicrobial ControlmedicineHumansMicrobial PathogensDisease burdenPharmacologyPrimary Health CareBacteriabusiness.industryPublic healthlcsh:ROrganismsBiology and Life SciencesCampylobactermedicine.diseaseTriageSurgeryIntestinal DiseasesSpecimen Preparation and TreatmentAntibiotic ResistanceFamily medicinelcsh:QAntimicrobial Resistancebusiness
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Becoming and being a biobank donor: The role of relationships and ethics

2020

Relational aspects, such as involvement of donor’s relatives or friends in the decision-making on participation in a research biobank, providing relatives’ health data to researchers, or sharing research findings with relatives should be considered when reflecting on ethical aspects of research biobanks. The aim of this paper is to explore what the role of donor’s relatives and friends is in the process of becoming and being a biobank donor and which ethical issues arise in this context. We performed qualitative analysis of 40 qualitative semi-structured interviews with biobank donors and researchers. The results show that relatedness to relatives or other types of close relationships playe…

MaleBiomedical ResearchEmotionsFace (sociological concept)Social SciencesFriendsResearch EthicsCognitionSociologyInformed consentMedicine and Health SciencesPsychologyHuman FamiliesResearch Integritymedia_commonBiological Specimen Banks0303 health sciencesMultidisciplinary030305 genetics & heredityQRPublic relationsResearch AssessmentMiddle AgedBiobankResearch PersonnelTissue DonorsGeneral Data Protection RegulationMedicineFemalePsychologyResearch ArticleMedical EthicsAdultScience PolicyScienceDecision MakingClinical Decision-MakingContext (language use)Research and Analysis Methods03 medical and health sciencesGeneticsmedia_common.cataloged_instanceHumansFamilyEuropean union030304 developmental biologyNutritionAgedResearch ethicsMotivationbusiness.industryCognitive PsychologyBiology and Life SciencesLatviaDietCognitive SciencebusinessMedical HumanitiesMedical ethicsNeurosciencePLoS ONE
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Optimizing clinical exome design and parallel gene-testing for recessive genetic conditions in preconception carrier screening: Translational researc…

2019

Limited translational genomic research data have been reported on the application of exome sequencing and parallel gene testing for preconception carrier screening (PCS). Here, we present individual-level data from a large PCS program in which exome sequencing was routinely performed on either gamete donors (5,845) or infertile patients (8,280) undergoing in vitro fertilization (IVF) treatment without any known family history of inheritable genetic conditions. Individual-level data on pathogenic variants were used to define conditions for PCS based on criteria for severity, penetrance, inheritance pattern, and age of onset. Fetal risk was defined based on actual carrier frequency data accou…

MaleCancer ResearchGenetic ScreensHeredityGenetic LinkageMolecular biologyGenetic Carrier ScreeningGene Identification and AnalysisGene SequencingQH426-470BioinformaticsPathology and Laboratory MedicineTranslational Research Biomedical0302 clinical medicineSequencing techniquesMedicine and Health SciencesExomeDNA sequencingGenome SequencingChildExomeGenetics (clinical)Exome sequencing0303 health scienceseducation.field_of_studymedicine.diagnostic_testGenetic Carrier ScreeningGenomicsPenetranceX-Linked TraitsSex LinkageChild PreschoolMedical geneticsFemalePathogensResearch ArticleAdultmedicine.medical_specialtyHeterozygotePopulationGenes RecessiveBiology03 medical and health sciencesGenomic MedicineDirected Tissue DonationExome SequencingmedicineGeneticsHumansGenetic Predisposition to DiseaseGenetic TestingeducationEcology Evolution Behavior and Systematics030304 developmental biologyGenetic testingClinical GeneticsGenome HumanInfant NewbornBiology and Life SciencesInfantHuman geneticsResearch and analysis methodsMolecular biology techniquesInfertilityGenetics of DiseaseMutation030217 neurology & neurosurgeryPLoS Genetics
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Criteria for the determination of maximal oxygen uptake in patients newly diagnosed with cancer : Baseline data from the randomized controlled trial …

2020

IntroductionMaximal oxygen uptake ([Formula: see text]) is a measure of cardiorespiratory fitness often used to monitor changes in fitness during and after treatment in cancer patients. There is, however, limited knowledge in how criteria verifying [Formula: see text] work for patients newly diagnosed with cancer. Therefore, the aim of this study was to describe the prevalence of fulfillment of typical criteria verifying [Formula: see text] and to investigate the associations between the criteria and the test leader's evaluation whether a test was performed "to exhaustion". An additional aim was to establish new cut-points within the associated criteria.MethodsFrom the Phys-Can randomized c…

MaleCancer Treatmentlaw.inventionCohort Studies0302 clinical medicineRandomized controlled trialHeart RateReference ValueslawNeoplasmsMedicine and Health SciencesPublic and Occupational HealthTreadmillRespiratory exchange ratioRating of perceived exertioneducation.field_of_studyMultidisciplinaryQRVO2 maxMiddle AgedVDP::Medisinske Fag: 700::Idrettsmedisinske fag: 850Sports ScienceExercise TherapyChemistryTreatment OutcomeOncologyCardiorespiratory FitnessResearch Design030220 oncology & carcinogenesisPhysical SciencesMedicineFemaleResearch ArticleChemical Elementsmedicine.medical_specialtySciencePhysical ExertionPopulationCardiologyResearch and Analysis Methods03 medical and health sciencesOxygen ConsumptionDiagnostic MedicineInternal medicineHeart rateCancer Detection and DiagnosismedicineHumansSports and Exercise MedicineeducationExerciseAgedColorectal Cancerbusiness.industryBiology and Life SciencesCancers and NeoplasmsCardiorespiratory fitnessPhysical Activity030229 sport sciencesOxygenROC CurvePhysical FitnessExercise Testbusinesshuman activities
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The ITA.LI.CA Staging System: A Novel Staging System for Hepatocellular Carcinoma

2016

Background Prognostic assessment in patients with hepatocellular carcinoma (HCC) remains controversial. Using the Italian Liver Cancer (ITA.LI.CA) database as a training set, we sought to develop and validate a new prognostic system for patients with HCC. Methods and Findings Prospective collected databases from Italy (training cohort, n = 3,628; internal validation cohort, n = 1,555) and Taiwan (external validation cohort, n = 2,651) were used to develop the ITA.LI.CA prognostic system. We first defined ITA.LI.CA stages (0, A, B1, B2, B3, C) using only tumor characteristics (largest tumor diameter, number of nodules, intra- and extrahepatic macroscopic vascular invasion, extrahepatic metas…

MaleCancer Treatmentlcsh:MedicineGastroenterologyMetastasisGeographical LocationsMathematical and Statistical Techniques0302 clinical medicineMedicine and Health SciencesEthnicitiesLiver DiseasesLiver NeoplasmsGeneral MedicinePrognosisItalian PeopleCancer treatmentOncology030220 oncology & carcinogenesisHepatocellular carcinomaPhysical SciencesFemale030211 gastroenterology & hepatologyalpha-FetoproteinsRadiologyLiver cancerStatistics (Mathematics)Research Articlemedicine.medical_specialtyCarcinoma HepatocellularAsiaSurgical and Invasive Medical ProceduresGastroenterology and HepatologyResearch and Analysis MethodsCarcinomasChild healthDigestive System Procedures03 medical and health sciencesDiagnostic MedicineInternal medicineGastrointestinal TumorsmedicineCarcinomaHumansStatistical MethodsStaging systemNeoplasm StagingTransplantationbusiness.industrylcsh:RCancers and NeoplasmsHepatocellular CarcinomaOrgan Transplantationmedicine.diseaseLiver TransplantationPeople and PlacesPopulation GroupingsNeoplasm stagingbusinessMathematicsForecastingPLoS Medicine
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Direct true lumen cannulation in type A acute aortic dissection: A review of an 11 years’ experience

2020

ObjectivesDirect true lumen cannulation (DTLC) of the aorta is an alternative cardiopulmonary bypass cannulation technique in the context of type A acute aortic dissection (A-AAD). DTLC has been reported to be effective in restoring adequate perfusion to jeopardized organs. This study reports and compares operative outcomes with DTLC or alternative cannulation techniques in a large cohort of patients with A-AAD.MethodsAll patients who underwent surgery for A-AAD between January 2006 and January 2017 in Mainz university hospital were reviewed. The choice of cannulation technique was left to the operating surgeon, however DTLC was our preference in patients who were in state of shock or showe…

MaleCardiac CatheterizationResuscitationCritical Care and Emergency MedicineCardiovascular ProceduresComputed Tomography AngiographyHealth Care ProvidersCannulationCardiovascular Medicine030204 cardiovascular system & hematologyCardiac CathetersDiagnostic Radiologylaw.inventionPostoperative Complications0302 clinical medicinelawMedicine and Health SciencesMedical PersonnelHospital MortalityProspective StudiesCardiovascular ImagingAortaAortic dissectionCardiopulmonary BypassMultidisciplinaryRadiology and ImagingQRAngiographyMiddle AgedAortic AneurysmSurvival RateProfessionsTreatment OutcomeMedicineFemaleTamponadeAnatomyResearch Articlemedicine.medical_specialtyDeath RatesImaging TechniquesScienceResuscitationCardiologyLumen (anatomy)Surgical and Invasive Medical ProceduresResearch and Analysis Methods03 medical and health sciencesAneurysmPopulation MetricsDiagnostic MedicinePhysiciansmedicineCardiopulmonary bypassCannulaHumansSurvival rateAgedRetrospective StudiesSurgeonsPopulation Biologybusiness.industryBiology and Life SciencesRetrospective cohort studymedicine.diseaseSurgeryHealth CareAortic Dissection030228 respiratory systemPeople and PlacesCardiovascular AnatomyBlood VesselsPopulation GroupingsbusinessPLOS ONE
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Troponin I Assay for Identification of a Significant Coronary Stenosis in Patients with Suspected Acute Myocardial Infarction and Wide QRS Complex

2016

Background Common ECG criteria such as ST-segment changes are of limited value in patients with suspected acute myocardial infarction (AMI) and bundle branch block or wide QRS complex. A large proportion of these patients do not suffer from an AMI, whereas those with ST-elevation myocardial infarction (STEMI) equivalent AMI benefit from an aggressive treatment. Aim of the present study was to evaluate the diagnostic information of cardiac troponin I (cTnI) in hemodynamically stable patients with wide QRS complex and suspected AMI. Methods In 417 out of 1818 patients presenting consecutively between 01/2007 and 12/2008 in a prospective multicenter observational study with suspected AMI a pro…

MaleCardiovascular ProceduresMyocardial Infarctionlcsh:MedicineCoronary Artery Disease030204 cardiovascular system & hematologyPathology and Laboratory MedicineBiochemistryVascular MedicineCoronary artery diseaseElectrocardiographyPatient Admission0302 clinical medicineTroponin IMedicine and Health SciencesMedicine030212 general & internal medicineMyocardial infarctionlcsh:ScienceStenosisMultidisciplinarymedicine.diagnostic_testbiologyMiddle AgedTroponinBioassays and Physiological Analysiscardiovascular systemCardiologyFemaleAlgorithmsResearch Articlemedicine.medical_specialtyCardiologySurgical and Invasive Medical ProceduresResearch and Analysis MethodsSensitivity and Specificity03 medical and health sciencesSigns and SymptomsDiagnostic MedicineInternal medicineHumansddc:610cardiovascular diseasesAgedCoronary RevascularizationBundle branch blockReceiver operating characteristicbusiness.industryRevascularizationElectrophysiological TechniquesAngioplastyTroponin Ilcsh:RCoronary StenosisBiology and Life SciencesProteinsmedicine.diseaseTroponinCytoskeletal Proteinsbiology.proteinMyocardial infarction complicationslcsh:QCardiac ElectrophysiologybusinessCoronary AngioplastyElectrocardiographyBiomarkersPLOS ONE
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Glia talk back.

2014

This study shows that the activity of neurons can trigger shedding of a protein, NG2, from the surface of oligodendrocyte precursor cells; this protein in turn modulates synaptic transmission, revealing a two-way conversation between neurons and glia.

MaleCell signalingNeural NetworksQH301-705.5AMPA receptorBiologyNeurotransmissionResearch and Analysis MethodsMechanical Treatment of SpecimensGeneral Biochemistry Genetics and Molecular BiologyBehavioral NeuroscienceNeurotransmitter receptormedicineBiological neural networkPremovement neuronal activityAnimalsBiology (General)AntigensNeuronsGeneral Immunology and MicrobiologyGeneral NeuroscienceMembrane ProteinsBiology and Life SciencesLong-term potentiationADAM ProteinsOligodendrogliamedicine.anatomical_structureElectroporationnervous systemSpecimen DisruptionSpecimen Preparation and TreatmentCellular NeuroscienceImmunologyProteoglycansNeuronAmyloid Precursor Protein SecretasesMolecular NeuroscienceGeneral Agricultural and Biological SciencesNeuroscienceResearch ArticleNeurosciencePLoS biology
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Naturalistic music and dance: Cortical phase synchrony in musicians and dancers

2018

Expertise in music has been investigated for decades and the results have been applied not only in composition, performance and music education, but also in understanding brain plasticity in a larger context. Several studies have revealed a strong connection between auditory and motor processes and listening to and performing music, and music imagination. Recently, as a logical next step in music and movement, the cognitive and affective neuro-sciences have been directed towards expertise in dance. To understand the versatile and overlapping processes during artistic stimuli, such as music and dance, it is necessary to study them with continuous naturalistic stimuli. Thus, we used long exce…

MaleCentral Nervous Systemkognitiomusic perceptionDancePhysiologytanssiEmotionsSocial Scienceslcsh:Medicinemusculoskeletal systemContemporary danceNervous Systemtuki- ja liikuntaelimetBAND0302 clinical medicinehavainnointiMedicine and Health SciencesPsychologymusic cognitionBRAINlcsh:SciencekiihtyvyysCerebral CortexClinical NeurophysiologyBrain MappingMultidisciplinaryMusic psychologyPhysics05 social sciencesClassical MechanicsSENSORIMOTOR SYNCHRONIZATIONMusic and movementFUNCTIONAL-ROLEElectrophysiologySilenceBioassays and Physiological AnalysisBrain ElectrophysiologyPhysical Sciencesta6131FemaleSensory PerceptionAnatomyPsychologyelectroencephalographyResearch ArticleCognitive psychologyAdult515 PsychologyImaging TechniqueskeskushermostomusiikkiNeurophysiologybioakustiikkaMIRROR-NEURON SYSTEMNeuroimagingContext (language use)Research and Analysis Methodsta3112050105 experimental psychologybioacousticsYoung Adult03 medical and health sciencesTHETA-SYNCHRONIZATIONtunteetOSCILLATIONSHumans0501 psychology and cognitive sciencesActive listeningDancingPERCEPTIONDESYNCHRONIZATIONElectrophysiological Techniqueslcsh:RCognitive PsychologyBiology and Life SciencesAcousticsaccelerationliikeMusic educationBrain WavesElectrophysiological PhenomenaALPHAaivokuoriAcoustic StimulationCognitive Science516 Educational scienceslcsh:QClinical MedicineMusic030217 neurology & neurosurgeryNeurosciencePLOS ONE
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