Search results for "BM"

Nutrition : des arômes pourraient très bien remplacer le sel

2018

The bitter taste of vitamins is mediated by human TAS2R activation

2021

Vitamins play an important role in maintaining normal physiologic and metabolic functions. Vitamins are known to generate bitterness, which may contribute to the off-taste of nutritional supplements. This negative sensation may decrease product acceptability and patient compliance. Few studies have examined taste thresholds and sensory sensing mechanisms at the origin of vitamin taste detection. To better understand vitamin bitterness perception, we combined human sensory analysis and taste receptor functional assays. We measured the aqueous taste detection threshold of four B vitamins using sensory difference tests. Challenging the 25 human bitter taste 2 receptors (TAS2Rs), we found that …

Chimio-détection, signalisation et intégration nerveuse chez la drosophile

2022

Techniques EEG et IRM pour l’étude de la flaveur chez l’humain

2022

Intramolecular signal transduction within the FixJ transcriptional activator: in vitro evidence for the inhibitory effect of the phosphorylatable reg…

1994

On line hydrogenation in GS-MS analysis of cyclic fatty acid monomers isolated from heated linseed oil

1989

International audience

Decoding sweet taste from chemical structures

2019

International audience

New technologies getting informations on repartition, orientation and identification of biomolecules on SPR chip

2008

We present herein an original approach, consisting to lead in parallel a global analysis (SPR) and nanoscale characterizations (AFM, mass spectrometry, TOF-SIMS) of bio-molecules on the biochip surface. The questions we want to answer concern especially the organization, orientation and identification of immobilized and captured biomolecules. In order to characterize the sensing layer in terms of molecular structure and orientation, we engaged a SPR-TOF SIMS first set of experiments (collaboration: Pr Aoyagi, Japan). Our results demonstrated that analysis of orientation of the immobilized cytochrome b5 is possible (ref2&3) and help to optimize and develop our sensing layer. We also recently…

Technological innovation around protein and cell biochip for diagnosis: a translational research from nanoworld to patient

2009

International audience; A great challenge in biosensors and diagnosis devices relies on the way to reconstitute relevant biological mechanisms on surface of the biochips and which analytical tools are convenient to provide accurate and rapid information on the structures and function of molecules attached to this surface. A better control in the realization of biochips can be obtained in combining different complementary approaches while always keeping in mind the biological key point. Researches in CLIPP are focused towards this objective. Conception, realization and characterization of protein and cell chips are presented. We detail different strategies of materials engineering1,2,3, chem…

Cell imaging connected with protein mapping to investigate glyphosate induced toxicity on human keratinocytes

2009

During the skin ageing which is accelerated by environmental factors, the generated reactive oxygen species make unstable the "proliferation/differentiation/apoptosis" coupling of epidermal cells. A biochemical study, previously realized on human keratinocyte cultures treated by a pesticide, glyphosate, allowed the validation of an in vitro model of skin ageing. In this work, we propose to study the loss of HaCaT cell integrity appearing after this chemical induced-oxidative stress. An original approach, combining a micro- to nanoscale cell characterization and analysis of fluctuating protein expression, is proposed: a) the exploration of cell growth and morphology through fluorescence and …