Search results for "BOTRYTIS CINEREA"
showing 10 items of 84 documents
Bacillus subtilis BS-2 and Peppermint Oil as Biocontrol Agents Against Botrytis cinerea
2019
The purpose of this study was to assess the activity of Bacillus subtilis BS-2 and peppermint oil against Botrytis cinerea. In this study parameters such as the age and the density of the bacterial culture and the incubation temperature were taken into consideration. Furthermore, the cellulolytic activity of the bacterium was determined. The effect of peppermint oil was evaluated at a concentration range of 0.5-4.0 %. The research was conducted with a dual culture plate method. The influence of B. subtilis BS-2 and peppermint oil on the growth of B. cinerea was evaluated based on the growth rate index. It was noted that the bacterial culture occurred at an initial density of OD560 = 1.0, cu…
Prenylated isoflavones from the stem bark of Erythrina sacleuxii
2018
Abstract Two new prenylated isoflavones, named erysacleuxin A (1) and B (2), along with eight known compounds, biochanin A (3), 5′-(3-methylbut-2-enyl)pratensein (4), 7-demethylrobustigenin (5), 3′-(3-methylbut-2-enyl)biochanin A (6) abyssinone V-4′-methyl ether (7), sigmoidin E (8), erythrinasinate (9), and burtinone (10) were isolated from the stem bark of Erythrina sacleuxii Hua (Leguminosae). The structures were determined based on spectroscopic and spectrometric data. All isolated compounds were evaluated for antifungal activity against Botrytis cinerea, Candida albicans, Eremothecium coryli, Penicillium notatum, Pyricularia oryzae, and Rhizomucor miehei with compounds 3, 4 and 6 showi…
Isolierung und Identifizierung von Sterinen im Metabolismus des Pilzes Botrytis cinerea / Isolation and identification of sterols in the metabolism o…
1983
The fungus Botrytis cinerea, which belongs to the class of ascomycetes, has been analysed for its sterol composition. It is able to produce ergosterol, cerevisterol, lanosterol/dihydrolanosterol and cholesterol besides β-sitosterol. The identification of the sterols is carried out with different analytical methods including mass spectrometry. In the extracts of the mycelium also squalene has been identified
Über die Bildung von Cholesterin durch Botrytis cinerea nach Lanosterinzugabe / The Production of Cholesterol by Botrytis cinerea after Addition of L…
1980
Abstract The fungus Botrytis cinerea, which is found on the grapes, is able to produce cholesterol after addition of lanosterol to the culture medium. The identification of cholesterol is carried out with different analytical methods including mass spectrometry. Under the same conditions ergosterol arises from squalene and not cholesterol.
Characterisation of a new species of Pythium isolated from a wheat field in northern France and its antagonism towards Botrytis cinerea causing the g…
2003
A new species, Pythium bifurcatum, isolated from soil samples taken from a wheat field in Lille in northern France is described here. The oomycete occurred thrice out of 50 samples. The type specimen is F-91, which is a slow-growing saprophyte living on vegetable debris and which can be recognised by its antheridial as well as oogonial characteristics, which are different from other known species of Pythium. When grown together with Botrytis cinerea, the causal agent of the grey mould disease of the grapevine, Pythium bifurcatum shows a pronounced antagonism and suppresses its growth. Morphological features of this new species, its antagonism to B. cinerea, the sequences of the ITS region o…
A Major Effect Gene Controlling Development and Pathogenicity in Botrytis cinerea Identified Through Genetic Analysis of Natural Mycelial Non-pathoge…
2021
Botrytis cinerea is a necrotrophic plant pathogenic fungus with a wide host range. Its natural populations are phenotypically and genetically very diverse. A survey of B. cinerea isolates causing gray mold in the vineyards of Castilla y León, Spain, was carried out and as a result eight non-pathogenic natural variants were identified. Phenotypically these isolates belong to two groups. The first group consists of seven isolates displaying a characteristic mycelial morphotype, which do not sporulate and is unable to produce sclerotia. The second group includes one isolate, which sporulates profusely and does not produce sclerotia. All of them are unresponsive to light. Crosses between a repr…
Etude du rôle du monoxyde d’azote (NO) dans la réponse du transcriptome d’Arabidopsis thaliana aux oligogalacturonides, un éliciteur des réactions de…
2012
SPEIPM; International audience; Le monoxyde d’azote (NO) est capable de réguler chez les plantes de nombreux processus physiologiques dont les réponses des plantes aux pathogènes. Peu d’informations sont disponibles aujourd’hui sur les mécanismes expliquant le rôle du NO endogène dans ce contexte physiologique. Grâce à une étude transcriptomique, nous avons caractérisé chez Arabidopsis thaliana, des gènes cibles du NO produit en réponse à un éliciteur des réactions de défense, les oligogalacturonides (OG). L’analyse a permis d’identifier parmi ces gènes cibles, un nombre important de gènes impliqués dans les réponses aux stress biotiques tels que des facteurs de transcription ou des protéin…
Preventive and post-infection control ofBotrytis cinereain tomato plants by hexanoic acid
2008
The antifungal activity of hexanoic acid on the phytopathogen Botrytis cinerea was studied. This chemical inhibited both spore germination and mycelial growth in vitro in a concentration- and pH-dependent manner, and stopped spore germination at a very early stage, preventing germ-tube development. The minimum fungicidal concentration (MFC) for in vitro spore germination was 16 m m . Hexanoic acid also inhibited in vitro mycelial growth of germinated spores at an MFC of 12 m m . Studies performed to characterize the mechanisms underlying the antimicrobial effect of hexanoic acid showed that it alters fungal membrane permeability. In addition, hexanoic acid treatment increased the levels of …
Characterization of a new, nonpathogenic mutant of Botrytis cinerea with impaired plant colonization capacity.
2006
International audience; Botrytis cinerea is a necrotrophic pathogen that attacks more than 200 plant species.Here, the nonpathogenic mutant A336, obtained via insertional mutagenesis, was characterized.Mutant A336 was nonpathogenic on leaves and fruits, on intact and wounded tissue, while still able to penetrate the host plant. It grew normally in vitro on rich media but its conidiation pattern was altered. The mutant did not produce oxalic acid and exhibited a modified regulation of the production of some secreted proteins (acid protease 1 and endopolygalacturonase 1). Culture filtrates of the mutant triggered an important oxidative burst in grapevine ( Vitis vinifera ) suspension cells, a…
Isolation frequency and efficiency of mycoparasitic Pythium species in the West Mediterranean region of Turkey
2021
Abstract A total of 132 mycoparasitic Pythium isolates were obtained from the soil samples taken from fruit orchards, vegetable fields, carnation greenhouses, sugarbeet fields and cereal fields in the West Mediterranean region of Turkey. The Oomycetes were isolated by using; surface soil dilution plate, sclerotia bait and precolonised plate methods. Isolates were identified as P. acanthophoron, P. amasculinum, P. lycopersicum, P. oligandrum, P. periplocum and P. paroecandrum, according to their morphological and molecular characteristics. The most commonly isolated mycoparasitic species were P. amasculinum and P. lycopersicum. The majority of the mycoparasites came from soil samples taken f…