Search results for "BRAF"
showing 10 items of 253 documents
The cellular composition and morphological organization of the ventricular zone in the adult zebrafish telencephalon
2009
Regenerative Medicine: Does Erythropoietin have a Role?
2009
Regenerative Medicine, a recent new medical domain, aims to develop new therapies through the stimulation of natural regenerative processes also in human beings. In this field, Erythropoietin (EPO) represents a significant subject of research. Several studies allow the assertion that EPO, in different concentrations, has protective effects mainly on the central nervous system, cardiovascular system and renal tissue. This action is carried out through one of few regenerative activities of human beings: angiogenesis. This mechanism, which involves endothelial stem cells and VEGF (Vascular Endothelial Growth Factor), has been experimentally demonstrated with Recombinant human erythropoietin (r…
Toxicity effects of the organic UV-filter 4-Methylbenzylidene camphor in zebrafish embryos
2019
Abstract Ultraviolet (UV) filters are widely used in personal care products and due to their lipophilicity these chemicals tend to bioaccumulate in the aquatic biota. 4-Methylbenzylidene camphor (4-MBC) is one of the most used UV-filters, and it is commonly detected in freshwater fish tissues. This substance is suspected to be an endocrine disruptor due to its interaction with Hypothalamus-Pituitary-Gonadal (HPG) and HP-Thyroid (HPT)-axis. The main objective of this study was to evaluate the effects of 4-MBC on apical endpoints, biochemical markers and on genes involved in endocrine pathways in Danio rerio. Zebrafish embryos were exposed to 4-MBC (0.083–0.77 mg/l) from 0 to 96 h post-fertil…
Acute hepatotoxicity induced by quetiapine fumarate in larval zebrafish
2016
Diferenciācijas indukcijas izpēte pēc BRAF un mTOR inhibitoru apstrādes BRAF mutētās ādas melanomas šūnās
2022
Darba mērķis bija padziļināti izpētīt BRAF inhibīcijas un sekojoši kombinētās BRAF un mTOR inhiibīcijas ietkemi uz metastātiskās BRAF mutēās melanomas šūnu līnijas SKMel28 šūnu ciklu, dzīvotspēju un inducēto atgriezensiko epitelilālo diferenciāciju. Ar advancētām mikroskopijas un šūnu analīzes metodēm tika apskatīti šos procesus iespējami ietkmējošie mehānismi. BRAF inhibīcija ar Vemurafenib rezultējās melanomas šūnu epiteliālā diferenciācijā un G1 arestā. Kombinētā BRAF un mTOR inhibīcija šūnās pagarināja šūnu diferencētā fenotipa stadiju, kas norāda uz iespējamu PI3K ceļa iesaisti SKMel28 šūnu augšanas un proliferācijas procesos. Lai arī pēc kombinētās BRAF-mTOR inhibīcijas diferencētais …
THE HDAC INHIBITOR ITF2357 (GIVINOSTAT) AS A KEY PLAYER IN EPIGENETIC TARGETING OF MELANOMA AND COLON CANCER CELLS
2023
Histone deacetylase inhibitors (HDACIs) are epigenetic compounds that have been recently considered for their promising anti-tumor activity. The aim of this PhD thesis was to elucidate and characterize the anti-tumor effect of the HDAC inhibitor ITF2357 (Givinostat) in melanoma and colon cancer cells that are characterized by oncogenic BRAF mutations. Interestingly, data reported in this thesis demonstrate that ITF2357 exerts a remarkable anti-tumor effect in melanoma cells by inducing a switch from a pro-survival autophagy to caspase-dependent apoptosis. The thesis provides the first evidences that ITF2357 is able to target oncogenic BRAF and oncogenic p53. The ITF2357 decreasing effect on…
Oncogenic BRAF and p53 Interplay in Melanoma Cells and the Effects of the HDAC Inhibitor ITF2357 (Givinostat)
2023
Oncogenic BRAF mutations have been widely described in melanomas and promote tumour progression and chemoresistance. We previously provided evidence that the HDAC inhibitor ITF2357 (Givinostat) targets oncogenic BRAF in SK-MEL-28 and A375 melanoma cells. Here, we show that oncogenic BRAF localises to the nucleus of these cells, and the compound decreases BRAF levels in both the nuclear and cytosolic compartments. Although mutations in the tumour suppressor p53 gene are not equally frequent in melanomas compared to BRAF, the functional impairment of the p53 pathway may also contribute to melanoma development and aggressiveness. To understand whether oncogenic BRAF and p53 may cooperate, a po…
An experimental approach to oxidative stress effect on sperm motility and DNA fragmentation
2018
En el estudio de la fertilidad masculina en todas las especies animales, la movilidad espermática, junto a la concentración y morfometría son los parámetros principales y más básicos en la evaluación de la calidad de los espermatozoides. La infertilidad masculina habitualmente se asocia con tasas anormales de estos parámetros seminales. Aún teniendo, los espermatozoides tasas adecuadas de todos estos parámetros no se puede garantizar la capacidad de fertilizar y/o desarrollar un cigoto. Por lo tanto, está claro que el examen rutinario del semen y los espermatozoides en la eyaculación no puede evaluar otros parámetros importantes en el proceso de fecundación como es capacitación espermática …
Visual Contrast Modulates Operant Learning Responses in Larval Zebrafish.
2018
The larval zebrafish is a promising vertebrate model organism to study neural mechanisms underlying learning and memory due to its small brain and rich behavioral repertoire. Here, we report on a high-throughput operant conditioning system for zebrafish larvae, which can simultaneously train 12 fish to associate a visual conditioned pattern with electroshocks. We find that the learning responses can be enhanced by the visual contrast, not the spatial features of the conditioned patterns, highlighted by several behavioral metrics. By further characterizing the learning curves as well as memory extinction, we demonstrate that the percentage of learners and the memory length increase as the co…