Search results for "BS"

showing 10 items of 20952 documents

Flexible multi-beam light-sheet fluorescence microscope for live imaging without striping artifacts

2018

The development of light-sheet fluorescence microscopy (LSFM) has greatly expanded the experimental capabilities in many biological and biomedical research fields, enabling for example live studies of murine and zebrafish neural activity or of cell growth and division. The key feature of the method is the selective illumination of a sample single plane, providing an intrinsic optical sectioning and allowing direct 2D image recording. On the other hand, this excitation scheme is more affected by absorption or scattering artifacts in comparison to point scanning methods, leading to un-even illumination. We present here an easily implementable method, based on acousto-optical deflectors (AOD),…

0301 basic medicineMaterials scienceOptical sectioningNeuroscience (miscellaneous)acousto optic deflectorbrain imagingAcousto optic deflector; Brain imaging; Fast volumetric imaging; Light-sheet fluorescence microscopy; Striping artifacts; Zebrafish; Anatomy; Neuroscience (miscellaneous); Cellular and Molecular Neurosciencelight-sheet fluorescence microscopy striping artifacts fast volumetric imaging acousto optic deflector brain imaging zebrafishfast volumetric imaginglcsh:RC321-571lcsh:QM1-69503 medical and health sciencesCellular and Molecular Neuroscience0302 clinical medicineOpticsLive cell imagingFluorescence microscopeTechnology ReportAbsorption (electromagnetic radiation)lcsh:Neurosciences. Biological psychiatry. Neuropsychiatrybusiness.industryScatteringlcsh:Human anatomyzebrafishSample (graphics)striping artifactsAcousto optic deflector Brain imaging Fast volumetric imaging Light-sheet fluorescence microscopy Striping artifacts Zebrafish Anatomy Neuroscience (miscellaneous) Cellular and Molecular Neurosciencelight-sheet fluorescence microscopy030104 developmental biologyFeature (computer vision)Light sheet fluorescence microscopyAnatomybusiness030217 neurology & neurosurgeryNeuroscience
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Surface structure promoted high-yield growth and magnetotransport properties of Bi2Se3 nanoribbons

2019

AbstractIn the present work, a catalyst-free physical vapour deposition method is used to synthesize high yield of Bi2Se3 nanoribbons. By replacing standard glass or quartz substrates with aluminium covered with ultrathin porous anodized aluminium oxide (AAO), the number of synthesized nanoribbons per unit area can be increased by 20–100 times. The mechanisms of formation and yield of the nanoribbons synthesized on AAO substrates having different arrangement and size of pores are analysed and discussed. It is shown that the yield and average length of the nanoribbons can base tuned by adjustment of the synthesis parameters. Analysis of magnetotransport measurements for the individual Bi2Se3…

0301 basic medicineMaterials scienceYield (engineering)Nanowirelcsh:Medicinechemistry.chemical_elementSubstrate (electronics)TOPOLOGICAL INSULATORArticleInorganic Chemistry03 medical and health scienceschemistry.chemical_compound0302 clinical medicineBI2TE3AluminiumMaterials ChemistryElectronic devices[CHIM]Chemical SciencesTopological insulatorsDEPOSITIONlcsh:ScienceNANOWIRESurface statesMultidisciplinaryAnodizingPOROUS ALUMINAlcsh:ROrganic ChemistrySynthesis and processingCondensed Matter PhysicsARRAYS030104 developmental biologychemistryChemical engineeringAluminium oxidelcsh:QLayer (electronics)030217 neurology & neurosurgeryScientific Reports
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Mucoadhesive solid lipid microparticles for controlled release of a corticosteroid in the chronic obstructive pulmonary disease treatment.

2017

Therapeutic efficacy of inhaled drugs is limited by rapid clearance from the site of action due to absorption into systemic circulation or metabolic degradation by alveolar macrophages. Drug delivery systems offer new solutions to clinical problems especially in the treatment of pulmonary diseases. In particular, Solid Lipid Microparticles (SLM) in the range of 3-5 µm are suggested as systems for delivery of therapeutics to the lung as, because of their size, they are able to deposit into secondary bronchi, avoiding systemic absorption typical of alveolar regions. Here, we describe two novel different SLMs prepared with chitosan and alginate for sustained release of fluticasone propionate (…

0301 basic medicineMedicine (miscellaneous)Biocompatible Materials02 engineering and technologyPharmacologymedicine.disease_causeChitosanPulmonary Disease Chronic Obstructivechemistry.chemical_compoundDrug StabilityGlucuronic AcidAdrenal Cortex HormonesMucoadhesive Solid Lipid Nanoparticles (SLMs);Aerodynamic diameter;Chronic obstructive pulmonary disease (COPD)General Materials Sciencechronic obstructive pulmonary disease (COPD)LungChromatography High Pressure LiquidDrug CarriersHexuronic Acidsaerodynamic diameter; chronic obstructive pulmonary disease (COPD); mucoadhesive solid lipid microparticles (SLMs)021001 nanoscience & nanotechnologyLipidsControlled releasemucoadhesive solid lipid microparticles (SLMs)Microspheresmedicine.anatomical_structureDrug deliveryCorticosteroid0210 nano-technologymedicine.drugBiocompatibilityAlginatesCell SurvivalSurface Propertiesmedicine.drug_classBiomedical EngineeringBioengineeringDevelopmentFluticasone propionate03 medical and health sciencesAdministration InhalationmedicineHumansParticle Sizeaerodynamic diameterChitosanLungbusiness.industryEpithelial CellsDrug LiberationOxidative Stress030104 developmental biologychemistryDelayed-Action PreparationsImmunologyMicroscopy Electron ScanningFluticasonebusinessOxidative stress
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High Fruit and Vegetable Consumption and Moderate Fat Intake Are Associated with Higher Carotenoid Concentration in Human Plasma

2021

This research was funded by CICYT [AGL2016-75329-R] and CIBEROBN from the Instituto de Salud Carlos III, ISCIII from the Ministerio de Ciencia, Innovación y Universidades, (AEI/FEDER, UE), Generalitat de Catalunya (GC) [2017SGR196]. The PREDIMED-Plus trial was supported by the official Spanish Institutions for funding scientific biomedical research, CIBER Fisiopatología de la Obesidad y Nutrición (CIBERobn) and Instituto de Salud Carlos III (ISCIII), through the Fondo de Investigación para la Salud (FIS), which is co-funded by the European Regional Development Fund (four coordinated Fondo de Investigaciones Sanitarias projects lead by J.S.-S. and J.V., including the following projects: PI13…

0301 basic medicineMediterranean dietFruit (Feed)PhysiologyClinical BiochemistryCarotenoidesLiquid chromatography030209 endocrinology & metabolismmatrix effect absorptionBiochemistryArticle03 medical and health sciences0302 clinical medicineMediterranean cookingFat intakeplasma carotenoidsMediterranean dietCuina mediterràniapolycyclic compoundsliquid chromatographyFood scienceCuina (Hortalisses)Molecular BiologyCarotenoidmass spectrometry2. Zero hungerchemistry.chemical_classificationConsumption (economics)030109 nutrition & dieteticsbioactive compoundsMass spectrometryorganic chemicalslcsh:RM1-950food and beveragesPREDIMED-Plus studyCell Biologydietary fatsphytochemicalsCarotenoidsbiological factorsCooking (Vegetables)3. Good healthlcsh:Therapeutics. PharmacologychemistryFruita (Aliment)Human plasmasense organsMatrix effect absorptionAntioxidants
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Molecular Mechanism of Inhibition of DNA Methylation by Zebularine

2017

In this work, we have analyzed the molecular mechanism of inhibition of a C5-DNA methyltransferase by zebularine using classical and QM/MM simulations. We found that the reaction proceeds with the addition of an unprotonated cysteine to the C6 position of the ring followed by methyl transfer to the C5 position. However, while the first step is reversible and presents a moderate free-energy barrier, the second step presents a large free-energy barrier, preventing the formation of the methylated complex. This mechanistic proposal agrees with recent experimental observations that point to the formation of a reversible covalent complex between DNA containing zebularine and methyltransferases. T…

0301 basic medicineMethyltransferaseStereochemistrySubstrate (chemistry)General ChemistryCatalysisQM/MM03 medical and health scienceschemistry.chemical_compound030104 developmental biologychemistryZebularineCovalent bondDNACytosineCysteineACS Catalysis
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The diagnosis of chronic endometritis in infertile asymptomatic women: a comparative study of histology, microbial cultures, hysteroscopy, and molecu…

2017

Background Chronic endometritis is a persistent inflammation of the endometrial mucosa caused by bacterial pathogens such as Enterobacteriaceae, Enterococcus, Streptococcus, Staphylococcus, Mycoplasma, and Ureaplasma. Although chronic endometritis can be asymptomatic, it is found in up to 40% of infertile patients and is responsible for repeated implantation failure and recurrent miscarriage. Diagnosis of chronic endometritis is based on hysteroscopy of the uterine cavity, endometrial biopsy with plasma cells being identified histologically, while specific treatment is determined based on microbial culture. However, not all microorganisms implicated are easily or readily culturable needing …

0301 basic medicineMicrobiological cultureBiopsyStaphylococcusChlamydia trachomatismedicine.disease_causeGastroenterologyUreaplasmaEndometriumGonorrhea0302 clinical medicineGardnerella vaginalisPathology MolecularAsymptomatic InfectionsEscherichia coli Infections030219 obstetrics & reproductive medicinebiologymedicine.diagnostic_testObstetrics and GynecologyHigh-Throughput Nucleotide SequencingBacterial InfectionsMiddle AgedStaphylococcal InfectionsGardnerella vaginalisMycoplasma hominisKlebsiella pneumoniaeFemaleEndometritisInfertility FemaleAdultDNA Bacterialmedicine.medical_specialtyPlasma CellsMycoplasma hominisHysteroscopyReal-Time Polymerase Chain ReactionSensitivity and Specificity03 medical and health sciencesYoung AdultMolecular microbiologyInternal medicineCulture TechniquesStreptococcal InfectionsmedicineEscherichia coliHumansMycoplasma InfectionsGram-Positive Bacterial Infectionsbusiness.industryStreptococcusSequence Analysis DNAChlamydia Infectionsbiology.organism_classificationNeisseria gonorrhoeaeKlebsiella Infections030104 developmental biologyChronic DiseasebusinessChronic EndometritisChlamydia trachomatisEnterococcusEndometrial biopsyAmerican journal of obstetrics and gynecology
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Genome-wide Association Study Identifies Genetic Variants Associated With Early and Sustained Response to (Pegylated) Interferon in Chronic Hepatitis…

2019

Wong, Grace LH/0000-0002-2863-9389; Wong, Vincent WS/0000-0003-2215-9410; Mangia, A/0000-0002-2600-3555; Brahmania, Mayur/0000-0002-4671-1479; Chan, Henry Lik-Yuen/0000-0002-7790-1611; Brouwer, Willem Pieter/0000-0001-8713-1481; Feld, Jordan/0000-0003-2640-2211; Tanwandee, Tawesak/0000-0001-7634-0843; Jaroszewicz, Jerzy/0000-0003-0139-4753; Chuaypen, Natthaya/0000-0002-5415-510X

0301 basic medicineMicrobiology (medical)AdultMaleHBsAgHepatitis B virusSettore MED/09 - Medicina InternaGenotyping TechniquesGenome-wide association studymedicine.disease_causePeripheral blood mononuclear cellAntiviral Agents03 medical and health sciences0302 clinical medicineHepatitis B ChronicSDG 3 - Good Health and Well-beingPegylated interferonInterferonmedicineHumansGWASchronic hepatitis BgeneticsProspective StudiespeginterferonArticles and CommentariesHepatitis B virusresponsebusiness.industryInterleukinInterferon-alphaMiddle Aged3. Good health030104 developmental biologyInfectious DiseasesHBeAgImmunologyMultivariate Analysis030211 gastroenterology & hepatologyFemaleInterferonsbusinessmedicine.drugGenome-Wide Association StudyClinical Infectious Diseases
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(1,3)-β-d-Glucan-based antifungal treatment in critically ill adults at high risk of candidaemia: an observational study.

2016

OBJECTIVES To determine the effects of a strategy that uses serum (1,3)-β-d-glucan (BDG) results for antifungal treatment of ICU patients at high risk of invasive candidiasis. PATIENTS AND METHODS Adult patients admitted to the ICU from January 2012 to June 2014 were included if they exhibited sepsis at the time of BDG testing and they met Candida score components ≥3. A retrospective analysis of collected data was performed. RESULTS In total, 198 patients were studied. Of 63 BDG-positive patients, 47 with candidaemia and 16 with probable Candida infection, all [31.8% (63/198)] received antifungal therapy. Of 135 BDG-negative patients, 110 [55.5% (110/198)] did not receive antifungal therapy…

0301 basic medicineMicrobiology (medical)AntifungalAdultMalemedicine.medical_specialtyAntifungal AgentsAntigens Fungalbeta-GlucansLetterAdolescentMedicine (all); Pharmacology; Infectious Diseases; Pharmacology (medical)medicine.drug_classCritical Illness030106 microbiologyAntifungal drugSettore MED/17 - MALATTIE INFETTIVESettore MED/07 - MICROBIOLOGIA E MICROBIOLOGIA CLINICAlaw.inventionSepsis03 medical and health sciencesYoung AdultlawInternal medicineSepsismedicineHumansPharmacology (medical)Candidiasis InvasiveMedical prescriptionYoung adultCandidaAgedRetrospective StudiesPharmacologyAged 80 and overbusiness.industryMedicine (all)Retrospective cohort studyMiddle Agedmedicine.diseaseIntensive care unitSurgeryInfectious DiseasesObservational studyFemaleProteoglycansbusinessThe Journal of antimicrobial chemotherapy
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Nitrogen Loss from Pristine Carbonate-Rock Aquifers of the Hainich Critical Zone Exploratory (Germany) Is Primarily Driven by Chemolithoautotrophic A…

2017

Despite the high relevance of anaerobic ammonium oxidation (anammox) for nitrogen loss from marine systems, its relative importance compared to denitrification has less been studied in freshwater ecosystems, and our knowledge is especially scarce for groundwater. Surprisingly, phospholipid fatty acids (PLFA)-based studies identified zones with potentially active anammox bacteria within two superimposed pristine limestone aquifer assemblages of the Hainich Critical Zone Exploratory (CZE; Germany). We found anammox to contribute an estimated 83% to total nitrogen loss in suboxic groundwaters of these aquifer assemblages at rates of 3.5-4.7 nmol L -1 d -1, presumably favored over denitrificati…

0301 basic medicineMicrobiology (medical)DenitrificationBrocadia fulgida030106 microbiologyved/biology.organism_classification_rank.specieslcsh:QR1-502chemistry.chemical_elementAquifersubsurfaceBiologyMicrobiologylcsh:Microbiology03 medical and health sciencesgroundwaterladderane lipidsOriginal Researchgeographydenitrificationgeography.geographical_feature_categoryved/biologyEcologyNitrite reductaseNitrogenchemolithoautotrophy030104 developmental biologychemistryMicrobial population biologyAnammoxanammoxNitrificationFrontiers in Microbiology
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Erratum for Yahav et al., "New β-Lactam-β-Lactamase Inhibitor Combinations".

2021

The limited armamentarium against drug-resistant Gram-negative bacilli has led to the development of several novel β-lactam–β-lactamase inhibitor combinations (BLBLIs). In this review, we summarize their spectrum of in vitro activities, mechanisms of resistance, and pharmacokinetic-pharmacodynamic (PK-PD) characteristics. A summary of available clinical data is provided per drug. Four approved BLBLIs are discussed in detail. All are options for treating multidrug-resistant (MDR) Enterobacterales and Pseudomonas aeruginosa. Ceftazidime-avibactam is a potential drug for treating Enterobacterales producing extended-spectrum β-lactamase (ESBL), Klebsiella pneumoniae carbapenemase (KPC), AmpC, a…

0301 basic medicineMicrobiology (medical)General Immunology and MicrobiologyCarbapenem resistantbiologyEpidemiologyChemistryStereochemistryKlebsiella pneumoniae030106 microbiologyPublic Health Environmental and Occupational HealthReviewbiochemical phenomena metabolism and nutritionbiology.organism_classificationbacterial infections and mycoses03 medical and health scienceschemistry.chemical_compound030104 developmental biologyInfectious Diseasesβ lactamase inhibitorLactampolycyclic compoundsbacteriaClinical microbiology reviews
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