6533b85afe1ef96bd12b9682
RESEARCH PRODUCT
Flexible multi-beam light-sheet fluorescence microscope for live imaging without striping artifacts
Natascia TisoVladislav GavryusevVladislav GavryusevGiuseppe VitoGiuseppe De VitoLapo TurriniLapo TurriniFrancesco VanziFrancesco VanziChiara FornettoChiara FornettoMassimiliano LocatelliMassimiliano LocatelliFrancesco S. PavoneFrancesco S. PavoneGiuseppe SancataldoGiuseppe SancataldoLudovico SilvestriLudovico Silvestrisubject
0301 basic medicineMaterials scienceOptical sectioningNeuroscience (miscellaneous)acousto optic deflectorbrain imagingAcousto optic deflector; Brain imaging; Fast volumetric imaging; Light-sheet fluorescence microscopy; Striping artifacts; Zebrafish; Anatomy; Neuroscience (miscellaneous); Cellular and Molecular Neurosciencelight-sheet fluorescence microscopy striping artifacts fast volumetric imaging acousto optic deflector brain imaging zebrafishfast volumetric imaginglcsh:RC321-571lcsh:QM1-69503 medical and health sciencesCellular and Molecular Neuroscience0302 clinical medicineOpticsLive cell imagingFluorescence microscopeTechnology ReportAbsorption (electromagnetic radiation)lcsh:Neurosciences. Biological psychiatry. Neuropsychiatrybusiness.industryScatteringlcsh:Human anatomyzebrafishSample (graphics)striping artifactsAcousto optic deflector Brain imaging Fast volumetric imaging Light-sheet fluorescence microscopy Striping artifacts Zebrafish Anatomy Neuroscience (miscellaneous) Cellular and Molecular Neurosciencelight-sheet fluorescence microscopy030104 developmental biologyFeature (computer vision)Light sheet fluorescence microscopyAnatomybusiness030217 neurology & neurosurgeryNeurosciencedescription
The development of light-sheet fluorescence microscopy (LSFM) has greatly expanded the experimental capabilities in many biological and biomedical research fields, enabling for example live studies of murine and zebrafish neural activity or of cell growth and division. The key feature of the method is the selective illumination of a sample single plane, providing an intrinsic optical sectioning and allowing direct 2D image recording. On the other hand, this excitation scheme is more affected by absorption or scattering artifacts in comparison to point scanning methods, leading to un-even illumination. We present here an easily implementable method, based on acousto-optical deflectors (AOD), to overcome this obstacle. We report the advantages provided by flexible and fast AODs in generating simultaneous angled multiple beams from a single laser beam and in fast light sheet pivoting and we demonstrate the suppression of illumination artifacts.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2018-10-01 |