Search results for "Bioinformatics"

showing 10 items of 1632 documents

MicroRNAs in solid organ and vascularized composite allotransplantation: Potential biomarkers for diagnosis and therapeutic use

2020

Abstract Nowadays, solid organ transplantation (SOT) is an established treatment for patients with end-organ dysfunction, which dramatically improves the quality-of-life. Vascularized composite allotransplants (VCAs) including hand and face have been reported worldwide over the last 20 years. However, VCAs, differently to SOT, are life-enhancing instead of life-saving and are not routinely performed due to the risk of immune rejection and the adverse effects of immunosuppression. Over the past decade, although considerable improvements in short-term outcomes after allotransplantation have been registered, these results have not been translated into major progress in long-term allograft acce…

Graft Rejectionmedicine.medical_specialtyImmune rejectionmedicine.medical_treatmentBioinformaticsVascularized Composite AllotransplantationOrgan transplantationRNA interferencemicroRNABiomarker; Immune rejection; Micro-RNAs; Organ transplantation; Tolerance; Vascularized composite allotransplantationsmedicineHumansBiomarker discoveryVascularized composite allotransplantationsImmunosuppression TherapyVascularized Composite AllotransplantationTransplantationOrgan transplantationbusiness.industryMicro-RNAsImmunosuppressionBiomarkerBiomarker (cell)MicroRNAsVascularized composite allotransplantations Micro-RNAs Biomarker Immune rejection Organ transplantation TolerancebusinessToleranceBiomarkersAllotransplantation
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Two Host Clades, Two Bacterial Arsenals: Evolution through Gene Losses in Facultative Endosymbionts.

2015

International audience; Bacterial endosymbiosis is an important evolutionary process in insects, which can harbor both obligate and facultative symbionts. The evolution of these symbionts is driven by evolutionary convergence, and they exhibit among the tiniest genomes in prokaryotes. The large host spectrum of facultative symbionts and the high diversity of strategies they use to infect new hosts probably impact the evolution of their genome and explain why they undergo less severe genomic erosion than obligate symbionts. Candidatus Hamiltonella defensa is suitable for the investigation of the genomic evolution of facultative symbionts because the bacteria are engaged in specific relations…

Hamiltonella defensaVirulence Factors[SDV]Life Sciences [q-bio]fungifood and beveragesGenomicscomparative genomicsbiochemical phenomena metabolism and nutritionBemisia tabaciEvolution MolecularHemipteraaphidsEnterobacteriaceaeCell WallAnimals[INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM]SymbiosisGene DeletionGenome BacterialPhylogenyResearch Article
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HBV recurrence after HCV clearance on DAAs: Sometimes they come back

2017

Hepatitis B virusHepatologybusiness.industryHcv clearanceHepacivirusHepatitis C ChronicHepatitis BBioinformaticsAntiviral AgentsHepatitis C03 medical and health sciencesHepatitis B Chronic0302 clinical medicineText mining030220 oncology & carcinogenesisHBVHumansMedicine030211 gastroenterology & hepatologybusinessJournal of Hepatology
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Identification of ELF3 as an early transcriptional regulator of human urothelium

2014

AbstractDespite major advances in high-throughput and computational modelling techniques, understanding of the mechanisms regulating tissue specification and differentiation in higher eukaryotes, particularly man, remains limited. Microarray technology has been explored exhaustively in recent years and several standard approaches have been established to analyse the resultant datasets on a genome-wide scale. Gene expression time series offer a valuable opportunity to define temporal hierarchies and gain insight into the regulatory relationships of biological processes. However, unless datasets are exactly synchronous, time points cannot be compared directly.Here we present a data-driven ana…

Hepatocyte Nuclear Factor 3-alphaTime seriesTime FactorsPPARγMicroarrayNormal Human UrotheliumComputational biologyBiologyReal-Time Polymerase Chain ReactionBioinformaticsProto-Oncogene ProteinsGene expressionElectric ImpedanceTranscriptional regulationHumansRNA Small InterferingGeneTranscription factorMolecular BiologyDNA PrimersGene knockdownProto-Oncogene Proteins c-etsReverse Transcriptase Polymerase Chain ReactionMicroarray analysis techniquesGene Expression Regulation DevelopmentalCell DifferentiationCell BiologyMicroarray AnalysisImmunohistochemistryELF3DNA-Binding ProteinsDifferentiationGene Knockdown TechniquesGene chip analysisGene expressionUrotheliumTranscription FactorsDevelopmental BiologyDevelopmental Biology
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SILAC labeling coupled to shotgun proteomics analysis of membrane proteins of liver stem/hepatocyte allows to candidate the inhibition of TGF-beta pa…

2014

Background: Despite extensive research on hepatic cells precursors and their differentiated states, much remains to be learned about the mechanism underlying the self-renewal and differentiation.Results: We apply the SILAC (stable isotope labeling by amino acids in cell culture) approach to quantitatively compare the membrane proteome of the resident liver stem cells (RLSCs) and their progeny spontaneously differentiated into epithelial/hepatocyte (RLSCdH). By means of nanoLC-MALDI-TOF/TOF approach, we identified and quantified 248 membrane proteins and 57 of them were found modulated during hepatocyte differentiation. Functional clustering of differentially expressed proteins by Ingenuity …

Hepatocyte differentiationProteomicsStem cellChemistryResearchLiver Stem CellProteomicProteomicsBioinformaticsBiochemistrySILACCell biologyMembrane proteinStable isotope labeling by amino acids in cell cultureTGF beta signaling pathwayHepatocyte; Proteomics; SILAC; Stem cell; Biochemistry; Molecular Biology[SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular BiologyHepatocyteStem cellShotgun proteomics[SDV.BDD]Life Sciences [q-bio]/Development BiologyMolecular BiologyProteome Science
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949 IDENTIFICATION OF TWO CLINICALLY DISTINCT SUBTYPES IN AUTOIMMUNE HEPATITIS BY COMPARATIVE FUNCTIONAL GENOMICS

2013

Conclusion: Incidence of autoimmune hepatitis is increasing, as other autoimmune diseases in developed countries. The main recognised hypothesis is the ‘hygienist theory’ (improvement of hygien leading to a decrease in infections) with few putative non exclusive mechanisms involving antigenic competition, extension of immune regulation induced by exogenous bacterial antigen or Toll Like Receptors.

HepatologyAntigenImmunologyImmune regulationmedicineIdentification (biology)Autoimmune hepatitisBacterial antigenBiologyReceptorBioinformaticsmedicine.diseaseFunctional genomicsJournal of Hepatology
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Azathioprine in inflammatory bowel disease: improved molecular insights and resulting clinical implications

2008

Azathioprine and 6-mercaptopurine represent important first-line immunosuppressive drugs in the treatment of inflammatory bowel disease. Owing to 45 years of clinical experience with thiopurines in inflammatory bowel disease, there currently exist strong data from numerous clinical trials and meta-analyses, which clearly document the therapeutic efficacy of azathioprine and 6-mercaptopurine in the treatment of inflammatory bowel disease. However, the exact molecular mechanism of action of these drugs was insufficiently understood for a long time. During the last few years, important new insights into the intracellular effects of azathioprine have been gained and thiopurines have been identi…

Hepatologybusiness.industryGastroenterologyAzathioprineInflammatory Bowel Diseasesmedicine.diseaseBioinformaticsInflammatory bowel diseaseClinical trialAzathioprineImmunologyMolecular mechanismHumansMedicinebusinessImmunosuppressive Agentsmedicine.drugExpert Review of Gastroenterology & Hepatology
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’-omics’ technology and human reproduction: reproductomics

2012

The success of assisted reproduction technology is highly dependent on a precise selection of gametes and embryos and determining the best endometrial window for embryo implantation. For many years, morphological criteria have constituted the only way to assess spermatozoon, oocytes, embryos and endometrial samples in order to improve outcomes. However, this approach has reached a ceiling of success and has been related to unacceptably high rates of multiple pregnancies. New technologies have been developed in order to improve the results and reduce risks via better selection of those gametes and embryos with the highest pregnancy potential, and of more favorable endometrial environments fo…

High ratemedicine.medical_specialtyPregnancyanimal structuresReproductive medicineObstetrics and GynecologyEmbryoBiologyOmicsBioinformaticsmedicine.diseaseTranscriptomeHuman reproductionMetabolomic profilingReproductive Medicineembryonic structuresMaternity and MidwiferyPediatrics Perinatology and Child HealthmedicineExpert Review of Obstetrics & Gynecology
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First Symposium of “Grupo Español de Investigación en Vesículas Extracelulares (GEIVEX)”, Segovia, 8–9 November 2012

2013

On the 8th and 9th of November, 71 scientists working in the field of extracellular vesicles from different laboratories in Spain met together in Segovia to present and discuss their research advances in this transformative and catalytic new field in translational biomedicine. (Published: 30 January 2013) Citation: Journal of Extracellular Vesicles 2013, 2 : 20256 - http://dx.doi.org/10.3402/jev.v2i0.20256

HistologyPolitical scienceLibrary scienceCell BiologyMeeting ReportBioinformaticsExtracellular vesiclesJournal of Extracellular Vesicles
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DNA and neutral lipid contents of peripheral cells as predictive biomarkers of mild cognitive impairment and Alzheimer's disease: Highlights on the i…

2014

Histologybusiness.industryCell BiologyDiseasemedicine.diseaseBioinformaticsPathology and Forensic MedicinePeripheralNeutral lipidchemistry.chemical_compoundchemistrymedicineDementiaAlzheimer's diseaseCognitive impairmentbusinessDNAPredictive biomarkerCytometry Part A
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