Search results for "Biostimulation"
showing 10 items of 11 documents
Successful aerobic bioremediation of groundwater contaminated with higher chlorinated phenols by indigenous degrader bacteria
2018
The xenobiotic priority pollutant pentachlorophenol has been used as a timber preservative in a polychlorophenol bulk synthesis product containing also tetrachlorophenol and trichlorophenol. Highly soluble chlorophenol salts have leaked into groundwater, causing severe contamination of large aquifers. Natural attenuation of higher-chlorinated phenols (HCPs: pentachlorophenol + tetrachlorophenol) at historically polluted sites has been inefficient, but a 4-year full scale in situ biostimulation of a chlorophenol-contaminated aquifer by circulation and re-infiltration of aerated groundwater was remarkably successful: pentachlorophenol decreased from 400 μg L−1 to <1 μg L−1 and tetrachlorophen…
Biostimulation proved to be the most efficient method in the comparison of in situ soil remediation treatments after a simulated oil spill accident
2016
The use of in situ techniques in soil remediation is still rare in Finland and most other European countries due to the uncertainty of the effectiveness of the techniques especially in cold regions and also due to their potential side effects on the environment. In this study, we compared the biostimulation, chemical oxidation, and natural attenuation treatments in natural conditions and pilot scale during a 16-month experiment. A real fuel spill accident was used as a model for experiment setup and soil contamination. We found that biostimulation significantly decreased the contaminant leachate into the water, including also the non-aqueous phase liquid (NAPL). The total NAPL leachate was …
BIODIVERSITA’ DELLE COMUNITA’ BATTERICHE IN UN SUOLO SOTTOPOSTO A BIORISANAMENTO IN MICROCOSMO
2021
Gli idrocarburi (HC) sono uno dei principali inquinanti ambientali. Una tecnologia promettente per il trattamento di siti contaminati si basa sulla biodegradazione degli inquinanti da parte di popolazioni microbiche capaci di utilizzare HC come fonte di carbonio ed energia (Biorisanamento). La necessità di risanare siti contaminati richiede da una parte la caratterizzazione delle comunità microbiche coinvolte nella biodegradazione, dall’altra lo sviluppo di interventi volti a migliorare il potenziale metabolico dei microrganismi degradatori. La nostra attività di ricerca ha avuto come obiettivo quello di caratterizzare e analizzare la biodiversità di comunità microbiche di un suolo contamin…
Removal of pharmaceuticals from municipal wastewaters at laboratory scale by treatment with activated sludge and biostimulation.
2017
Abstract Municipal wastewater containing 21 pharmaceutical compounds, as well as activated sludge obtained from the aeration tank of the same wastewater treatment plant were used in lab-scale biodegradation experiments. The concentrations of pharmaceutical compounds were determined by high-performance liquid chromatography coupled to Orbitrap high-resolution mass spectrometry and ranged from 13.2 ng/L to 51.8 μg/L. Activated sludge was characterized in the terms of phylogenetic and catabolic diversity of microbial community, as well as its morphology. Proteobacteria (24.0%) represented the most abundant phylum, followed by Bacteroidetes (19.8%) and Firmicutes (13.2%). Bioaugmentation of was…
MICROBIAL COMMUNITY CHANGES IN TNT SPIKED SOIL BIOREMEDIATION TRIAL USING BIOSTIMULATION, PHYTOREMEDIATION AND BIOAUGMENTATION
2013
Trinitrotoluene (TNT), a commonly used explosive for military and industrial applications, can cause serious environmental pollution. 28-day laboratory pot experiment was carried out applying bioaugmentation using laboratory selected bacterial strains as inoculum, biostimulation with molasses and cabbage leaf extract, and phytoremediation using rye and blue fenugreek to study the effect of these treatments on TNT removal and changes in soil microbial community responsible for contaminant degradation. Chemical analyses revealed significant decreases in TNT concentrations, including reduction of some of the TNT to its amino derivates during the 28-day tests. The combination of bioaugmentation…
The role of nutrients in the biodegradation of 2,4,6-trinitrotoluene in liquid and soil.
2011
The widely used explosive 2,4,6-trinitrotoluene (TNT) has residues that are potentially explosive, toxic, and mutagenic. TNT and other explosives can be degraded by microorganisms; however, biostimulation is needed for process efficiency. To investigate the effectiveness of using biostimulation to degrade TNT, we added varying concentrations of a nutrient amendment consisting of inorganic salts, plant extracts, and molasses to soil and liquid media. For the inoculum we used a consortium of bacteria AM 06 that had exhibited the ability to degrade TNT and which had been previously isolated from explosives-contaminated soils. Phylogenetically, the clones clustered into seven different genera: …
Evidence for shifts in the structure and abundance of the microbial community in a long-term PCB-contaminated soil under bioremediation.
2011
International audience; Although the impact of bioremediation of PCB-contaminated sites on the indigenous microbial community is a key question for soil restoration, it remains poorly understood. Therefore, a small-scale bioremediation assay made of (a) a biostimulation treatment with carvone, soya lecithin and xylose and (b) two bioaugmentation treatments, one with a TSZ7 mixed culture and another with a Rhodococcus sp. Z6 pure strain was set up. Changes in the structure of the global soil microbial community and in the abundances of different taxonomic phyla were monitored using ribosomal intergenic spacer analysis (RISA) and real-time PCR. After an 18-month treatment, the structure of th…
Analysis of Chemical and Biochemical Parameters of Petrol-Contaminated Soil after Biostimulation with an Enzyme Reagent
2020
This study aimed to assess the effect of petrol and the Fyre Zyme reagent on selected chemical and biochemical properties of loamy sand. The experiment was conducted under laboratory conditions. First, petrol was introduced into the soil at doses of 0 and 50 g k-1dry matter (DM). Next, 6% Fyre-Zyme enzyme reagent solution was added to the samples contaminated and uncontaminated with petrol, in the following combinations: 0 (control), once at 40 cm3 kg&ndash
Biostimulation of in situ microbial degradation processes in organically-enriched sediments mitigates the impact of aquaculture
2019
Fish farm deposition, resulting in organic matter accumulation on bottom sediments, has been identified as among the main phenomena causing negative environmental impacts in aquaculture. An in situ bioremediation treatment was carried out in order to reduce the organic matter accumulation in the fish farm sediments by promoting the natural microbial biodegradation processes. To assess the effect of the treatment, the concentration of organic matter in the sediment and its microbial degradation, as well as the response of the benthic prokaryotic community, were investigated. The results showed a significant effect of the treatment in stimulating microbial degradation rates, and the consequen…
Evaluation of effectiveness of photobiostimulation in alleviating side effects after dental implant surgery. A randomized clinical trial
2020
Background To assess if photobiostimulation (PBS) alleviates pain intensity/duration and swelling after implant surgery. Material and Methods Sixty subjects (27 male and 33 female, with a mean age of 47,13 8.05 years) were included and randomly assigned to experimental group (implant surgery and photobiostimulation), placebo group (implant surgery and simulated photobiostimulation) and control group (implant surgery only). Inclusion criteria: subjects older than 20 years, with a healthy oral mucosa and requiring implant surgery. Exclusion criteria: pregnancy, history of implant failure, light sensitivity, metabolic deseases, consumption of antibiotics or corticosteroids in the last two week…