Search results for "Blood cells"

showing 10 items of 116 documents

Circulating monocyte subsets and heart failure prognosis

2018

Altres ajuts: Fundacio' La MARATO' de TV3 (201502 to ABG, 201516), AdvanceCat with the support of ACCIÓ [Catalonia Trade & Investment, Generalitat de Catalunya], Fundació Bancària La Caixa. Monocytes are a heterogeneous population of effector cells with key roles in tissue integrity restoration and maintenance. Here, we explore the association of monocyte subsets and prognosis in patients with ambulatory heart failure (HF). Monocyte subsets were classified as classical (CD14 ++ /CD16 -), intermediate (CD14 ++ /CD16 +), or non-classical (CD14 + /CD16 ++). Percentage distribution and absolute cell count were assessed in each subset, and multivariable Cox regression analyses were performed wit…

Male0301 basic medicineEtiologylcsh:Medicine030204 cardiovascular system & hematologyPathology and Laboratory MedicineMonocytesWhite Blood CellsMathematical and Statistical Techniques0302 clinical medicineAnimal CellsCell MovementRisk FactorsCause of DeathMedicine and Health Scienceslcsh:ScienceCause of deathMultidisciplinaryEjection fractionHeartPrognosismedicine.anatomical_structurePhysical SciencesAmbulatoryCardiologyRegression AnalysisFemaleCellular TypesAnatomyStatistics (Mathematics)Research Articlemedicine.medical_specialtyImmune CellsCD14ImmunologyCell Enumeration TechniquesCardiologyResearch and Analysis MethodsDisease-Free Survival03 medical and health sciencesDiagnostic MedicineInternal medicinemedicineHumansStatistical MethodsAgedProportional Hazards ModelsHeart FailureBlood Cellsbusiness.industryProportional hazards modelMonocytelcsh:RBiology and Life SciencesCell Biologymedicine.disease030104 developmental biologyVentricleHeart failureCardiovascular Anatomylcsh:QbusinessMathematicsEjection FractionPLOS ONE
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Impact of Consuming Extra-Virgin Olive Oil or Nuts within a Mediterranean Diet on DNA Methylation in Peripheral White Blood Cells within the PREDIMED…

2017

DNA methylation could be reversible and mouldable by environmental factors, such as dietary exposures. The objective was to analyse whether an intervention with two Mediterranean diets, one rich in extra-virgin olive oil (MedDiet + EVOO) and the other one in nuts (MedDiet + nuts), was influencing the methylation status of peripheral white blood cells (PWBCs) genes. A subset of 36 representative individuals were selected within the PREvención con DIeta MEDiterránea (PREDIMED-Navarra) trial, with three intervention groups in high cardiovascular risk volunteers: MedDiet + EVOO, MedDiet + nuts, and a low-fat control group. Methylation was assessed at baseline and at five-year follow-up. Ingenui…

Male0301 basic medicineTime FactorsMediterranean diet; DNA methylation; nuts; olive oil; blood cellsMediterranean dietADNPhysiologyComorbidity030204 cardiovascular system & hematologyDiet MediterraneanEpigenesis Genetic0302 clinical medicineRisk FactorsLeukocytesNutsCooking (Dried foods)Aged 80 and overchemistry.chemical_classificationDNA methylationNutrition and DieteticsMethylationMiddle AgedOli d'olivaTreatment OutcomeCpG siteCardiovascular DiseasesDNA methylationFemaleDiet HealthyMetilaciólcsh:Nutrition. Foods and food supplyPolyunsaturated fatty acidBlood cellslcsh:TX341-641nutsBiologyMethylationArticle03 medical and health sciencesMediterranean cookingMediterranean dietDiabetes mellitusCuina mediterràniamedicineHumansEpigeneticsOlive OilAgedMetabolismDNAProtective Factorsmedicine.diseaseCuina (Fruita seca)030104 developmental biologychemistrySpainCèl·lules sanguíniesCpG Islandsblood cellsEnergy MetabolismOlive oilFood Science
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Blood cell transcript levels in 5-year-old children as potential markers of breastfeeding effects in those small for gestational age at birth

2019

BackgroundNutrition of the newborn during the early postnatal period seems to be of capital importance and there is clinical evidence showing the protective effect of breastfeeding compared with formula feeding on childhood obesity and its comorbidities. Infants born small for gestation age may be more sensitive to the type of feeding during lactation. Here, we aimed to analyze the impact of birth weight and the type of infant feeding on the expression levels in peripheral blood cells of selected candidate genes involved in energy homeostasis in 5-year-old children, to find out potential early biomarkers of metabolic programming effects during this period of metabolic plasticity.MethodsFort…

Male0301 basic medicinehumanosBreastfeedingPhysiologylcsh:MedicineSangBlood Pressureregulación de la expresión génicalactancia materna0302 clinical medicineLactationBirth Weightcélulas sanguíneasGeneral MedicineARNmedicine.anatomical_structureBreast FeedingChild Preschool030220 oncology & carcinogenesisInfant Small for Gestational AgeInfant formulaGestationFemaleInfantspresión sanguíneaBreast milkSystoleBirth weightBreast milkGeneral Biochemistry Genetics and Molecular BiologyChildhood obesity03 medical and health sciencessístoleMetabolic programmingmedicineHumansRNA Messengerpeso al nacerlactanteBlood Cellsbusiness.industryResearchlcsh:RInfant NewbornInfantmedicine.diseaseCardiometabolic risk factors030104 developmental biologyInfant formulaGene Expression RegulationSmall for gestational ageRNAbusinessBiomarkers
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Heterogeneity of circulating CD8 T-cells specific to islet, neo-antigen and virus in patients with type 1 diabetes mellitus

2018

Auto-reactive CD8 T-cells play an important role in the destruction of pancreatic β-cells resulting in type 1 diabetes (T1D). However, the phenotype of these auto-reactive cytolytic CD8 T-cells has not yet been extensively described. We used high-dimensional mass cytometry to phenotype autoantigen- (pre-proinsulin), neoantigen- (insulin-DRIP) and virus- (cytomegalovirus) reactive CD8 T-cells in peripheral blood mononuclear cells (PBMCs) of T1D patients. A panel of 33 monoclonal antibodies was designed to further characterise these cells at the single-cell level. HLA-A2 class I tetramers were used for the detection of antigen-specific CD8 T-cells. Using a novel Hierarchical Stochastic Neighb…

Male0301 basic medicinelcsh:MedicineCD8-Positive T-LymphocytesBiochemistryAutoantigensEndocrinologyInsulin-Secreting CellsCellular typesCytotoxic T celllcsh:ScienceStainingMultidisciplinaryImmune cellsCell StainingPhenotypePhenotypesData AcquisitionPhenotypeWhite blood cellsFemaleSingle-Cell AnalysisResearch ArticleAdultCell biologyBlood cellsComputer and Information SciencesEndocrine Disordersmedicine.drug_classImmunologyT cellsCytotoxic T cellsBiologyResearch and Analysis MethodsMonoclonal antibodyPeripheral blood mononuclear cellVirus03 medical and health sciencesHLA-A2 AntigenGeneticsDiabetes MellitusmedicineHumansMass cytometryMedicine and health sciencesType 1 diabetesBiology and life scienceslcsh:Rmedicine.diseaseDiabetes Mellitus Type 1030104 developmental biologyAnimal cellsSpecimen Preparation and TreatmentMetabolic DisordersImmunologyLeukocytes Mononuclearlcsh:QCytometryBiomarkersCD8
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Fatty Acids Composition of Blood Cell Membranes and Peripheral Inflammation in the PREDIMED Study: A Cross-Sectional Analysis

2019

There is limited evidence from epidemiological studies for the inflammatory or anti-inflammatory properties of fatty acids in blood cell membranes. Therefore, this study examined associations between baseline (n = 282) and 1-year (n = 143) changes in the levels of fatty acids in blood cell membranes with circulating inflammatory markers in older adults at high cardiovascular risk. The data for this cross-sectional analysis was obtained from a case-control study within the PREDIMED study. Linear regression with elastic net penalty was applied to test associations between measured fatty acids and inflammatory markers. Several fatty acids were associated with interferon-&gamma

Male0301 basic medicinemedicine.medical_specialtyCross-sectional studyÀcids grassoslcsh:TX341-641Inflammation030204 cardiovascular system & hematologyfatty acidsArticleBlood cell03 medical and health sciences0302 clinical medicine:Ciencias de la Salud::Medicina preventiva [Materias Investigacion]Internal medicinemedicineHumansFatty acidsAgedcell membranesAged 80 and overInflammationBlood Cells030109 nutrition & dieteticsNutrition and DieteticsChemistryCell MembraneInterleukinMiddle AgedPredimedPeripheralCell membranesCross-Sectional StudiesMembraneEndocrinologymedicine.anatomical_structureinflammationCase-Control StudiesCytokinesFemaleFatty acid compositionmedicine.symptomlcsh:Nutrition. Foods and food supplyMembranes cel·lularsFood ScienceNutrients
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Antilymphocyte Globulin for Prevention of Chronic Graft-versus-Host Disease.

2016

Clinical Trial, Phase III; Journal Article; Multicenter Study; Randomized Controlled Trial; Research Support, Non-U.S. Gov't; BACKGROUND Chronic graft-versus-host disease (GVHD) is the leading cause of later illness and death after allogeneic hematopoietic stem-cell transplantation. We hypothesized that the inclusion of antihuman T-lymphocyte immune globulin (ATG) in a myeloablative conditioning regimen for patients with acute leukemia would result in a significant reduction in chronic GVHD 2 years after allogeneic peripheral-blood stem-cell transplantation from an HLA-identical sibling. METHODS We conducted a prospective, multicenter, open-label, randomized phase 3 study of ATG as part of …

Male:Analytical Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Statistics as Topic::Models Statistical::Proportional Hazards Models [Medical Subject Headings]T-LymphocytesPhases of clinical researchGraft vs Host Disease:Named Groups::Persons::Age Groups::Adult::Middle Aged [Medical Subject Headings]Linfocitos t:Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings]Immunosuppressive Agent0302 clinical medicineTrasplante homólogoEstudios prospectivosMedicineCumulative incidenceProspective StudiesProspective cohort studyChildTransplantation Homologou:Chemicals and Drugs::Amino Acids Peptides and Proteins::Proteins::Blood Proteins::Immunoproteins::Immunoglobulins::Antibodies::Immune Sera::Antilymphocyte Serum [Medical Subject Headings]Acute leukemia:Anatomy::Cells::Blood Cells::Leukocytes::Leukocytes Mononuclear::Lymphocytes::T-Lymphocytes [Medical Subject Headings]:Diseases::Pathological Conditions Signs and Symptoms::Pathologic Processes::Disease Attributes::Chronic Disease [Medical Subject Headings]:Named Groups::Persons::Age Groups::Child::Child Preschool [Medical Subject Headings]Medicine (all)IncidenceSuero antilinfocíticoGeneral MedicineMiddle AgedModelos de riesgos proporcionales3. Good healthHumanosSurvival Rate030220 oncology & carcinogenesis:Named Groups::Persons::Age Groups::Adolescent [Medical Subject Headings]Child PreschoolFemale:Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Physiological Effects of Drugs::Immunologic Factors::Immunosuppressive Agents [Medical Subject Headings]Immunosuppressive AgentsHumanHomologousAdultmedicine.medical_specialty:Analytical Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Epidemiologic Study Characteristics as Topic::Epidemiologic Studies::Cohort Studies::Longitudinal Studies::Prospective Studies [Medical Subject Headings]:Named Groups::Persons::Age Groups::Adult::Young Adult [Medical Subject Headings]AdolescentEnfermedad injerto contra huéspedDisease-Free Survival03 medical and health sciencesYoung AdultInternal medicine:Named Groups::Persons::Age Groups::Adult [Medical Subject Headings]HumansTransplantation HomologousSupervivencia sin enfermedadPreschoolSurvival rate:Analytical Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Prognosis::Disease-Free Survival [Medical Subject Headings]:Named Groups::Persons::Age Groups::Child [Medical Subject Headings]Antilymphocyte SerumProportional Hazards ModelsTransplantationbusiness.industryEnfermedad crónicamedicine.diseaseInmunosupresoresAnti-thymocyte globulinSurgeryTransplantation:Diseases::Immune System Diseases::Graft vs Host Disease [Medical Subject Headings]Prospective Studie:Analytical Diagnostic and Therapeutic Techniques and Equipment::Surgical Procedures Operative::Transplantation::Transplantation Homologous [Medical Subject Headings]Graft-versus-host diseaseT-Lymphocyte:Check Tags::Female [Medical Subject Headings]Chronic DiseaseProportional Hazards ModelAdolescent; Adult; Antilymphocyte Serum; Child; Child Preschool; Chronic Disease; Disease-Free Survival; Female; Graft vs Host Disease; Humans; Immunosuppressive Agents; Incidence; Male; Middle Aged; Proportional Hazards Models; Prospective Studies; Survival Rate; T-Lymphocytes; Transplantation Homologous; Young Adult; Medicine (all)business030215 immunology
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The nutrigenetic influence of the interaction between dietary vitamin E and TXN and COMT gene polymorphisms on waist circumference: a case control st…

2015

Background Abdominal obesity (AO) is a common modifiable risk factor for certain non-communicable diseases associated with enhanced oxidative stress (OS). The objective of this work was to investigate whether the interaction between antioxidant vitamin intake and OS-related polymorphisms modulates gene-associated anthropometry in a Spanish population. Methods A total of 246 subjects with AO, and 492 age and gender matched non-AO subjects were included in the study. Anthropometric, biochemical, and OS parameters, and antioxidant dietary intake data were assessed using validated procedures. DNA from white blood cells was isolated and the genotype of seven polymorphisms from genes involved in …

MaleAntioxidantPolimorphismmedicine.medical_treatment:Chemicals and Drugs::Amino Acids Peptides and Proteins::Proteins::Thioredoxins [Medical Subject Headings]Antioxidantes:Analytical Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Statistics as Topic::Probability::Risk::Risk Factors [Medical Subject Headings]AntioxidantsVitamin E intakeObesidad abdominalchemistry.chemical_compoundNutrigenomicsThioredoxinsPolymorphism (computer science):Anatomy::Cells::Blood Cells::Leukocytes [Medical Subject Headings]Risk FactorsGenotypeVitamin E:Chemicals and Drugs::Nucleic Acids Nucleotides and Nucleosides::Nucleosides::Deoxyribonucleosides::Deoxyguanosine [Medical Subject Headings]:Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Physiological Effects of Drugs::Growth Substances::Micronutrients::Vitamins [Medical Subject Headings]:Phenomena and Processes::Metabolic Phenomena::Metabolism::Oxidative Stress [Medical Subject Headings]Abdominal obesityNutrigenómicaMedicine(all)AnthropometryAge FactorsGeneral MedicineAbdominal obesity:Phenomena and Processes::Physiological Phenomena::Body Constitution::Body Weights and Measures::Body Size::Body Weight::Overweight::Obesity::Obesity Abdominal [Medical Subject Headings]Middle Aged:Analytical Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Statistics as Topic::Models Statistical::Logistic Models [Medical Subject Headings]Waist circumferenceDietaFemale:Phenomena and Processes::Genetic Phenomena::Genotype [Medical Subject Headings]medicine.symptomFactores de riesgoVitaminAdultmedicine.medical_specialtyGenotypeVitamina ECatechol-O-methyltransferaseBiology:Phenomena and Processes::Physiological Phenomena::Nutritional Physiological Phenomena::Diet [Medical Subject Headings]:Phenomena and Processes::Genetic Phenomena::Genetic Structures::Genome::Genome Components::Genes::Genes Reporter [Medical Subject Headings]Catechol O-Methyltransferase:Chemicals and Drugs::Biological Factors::Pigments Biological::Carotenoids::Retinoids::Vitamin A [Medical Subject Headings]Polymorphism Single NucleotideGeneral Biochemistry Genetics and Molecular BiologySex FactorsInternal medicine:Chemicals and Drugs::Inorganic Chemicals::Oxygen Compounds::Reactive Oxygen Species [Medical Subject Headings]:Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Physiological Effects of Drugs::Protective Agents::Antioxidants [Medical Subject Headings]medicinePerímetro abdominal:Phenomena and Processes::Physiological Phenomena::Body Constitution::Body Weights and Measures::Body Size::Waist Circumference [Medical Subject Headings]:Chemicals and Drugs::Heterocyclic Compounds::Heterocyclic Compounds 2-Ring::Benzopyrans::Vitamin E [Medical Subject Headings]HumansObesityPolymorphismThioredoxinAgedCatechol-O-methyl transferaseBiochemistry Genetics and Molecular Biology(all)Vitamin EResearchCase-control studyGenes informadores:Disciplines and Occupations::Natural Science Disciplines::Biological Science Disciplines::Biology::Genetics::Genomics::Nutrigenomics [Medical Subject Headings]DietOxidative StressEndocrinologychemistrySpainOxidative stressCase-Control Studies:Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Transferases::One-Carbon Group Transferases::Methyltransferases::Catechol O-Methyltransferase [Medical Subject Headings]:Chemicals and Drugs::Nucleic Acids Nucleotides and Nucleosides::Nucleic Acids::DNA [Medical Subject Headings]Genotipo
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Synergistic effect between amoxicillin and TLR ligands on dendritic cells from amoxicillin-delayed allergic patients.

2013

Journal Article; Amoxicillin, a low-molecular-weight compound, is able to interact with dendritic cells inducing semi-maturation in vitro. Specific antigens and TLR ligands can synergistically interact with dendritic cells (DC), leading to complete maturation and more efficient T-cell stimulation. The aim of the study was to evaluate the synergistic effect of amoxicillin and the TLR2, 4 and 7/8 agonists (PAM, LPS and R848, respectively) in TLR expression, DC maturation and specific T-cell response in patients with delayed-type hypersensitivity (DTH) reactions to amoxicillin. Monocyte-derived DC from 15 patients with DTH to amoxicillin and 15 controls were cultured with amoxicillin in the pr…

MaleCélulas dendríticasmedicine.medical_treatmentLymphocyte proliferationPharmacology:Chemicals and Drugs::Chemical Actions and Uses::Specialty Uses of Chemicals::Laboratory Chemicals::Ligands [Medical Subject Headings]Monocytes:Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings]:Phenomena and Processes::Physiological Phenomena::Pharmacological Phenomena::Pharmacological Processes::Drug Interactions::Drug Synergism [Medical Subject Headings]Cells CulturedAmoxicilinaMultidisciplinarymedicine.diagnostic_testChemistryQRLinfocitosImidazolesCitocinasMiddle AgedHumanosCytokineMedicineCytokinesFemaleDrug EruptionsResearch Article:Diseases::Skin and Connective Tissue Diseases::Skin Diseases::Exanthema [Medical Subject Headings]AdultSinergismo medicamentosoScienceFlow cytometryHipersensibilidad retardada:Chemicals and Drugs::Organic Chemicals::Amides::Lactams::beta-Lactams::Penicillins::Penicillin G::Ampicillin::Amoxicillin [Medical Subject Headings]Immune systemAntigen:Diseases::Immune System Diseases::Hypersensitivity::Hypersensitivity Delayed [Medical Subject Headings]ExantemamedicineHypersensitivity:Anatomy::Cells::Blood Cells::Leukocytes::Leukocytes Mononuclear::Lymphocytes [Medical Subject Headings]HumansLigandos:Chemicals and Drugs::Amino Acids Peptides and Proteins::Peptides::Intercellular Signaling Peptides and Proteins::Cytokines [Medical Subject Headings]:Anatomy::Cells::Antigen-Presenting Cells::Dendritic Cells [Medical Subject Headings]TLR9AmoxicillinTLR7Dendritic CellsToll-Like Receptor 2TLR2ImmunologyPloS one
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Analysis of Mycobacterium tuberculosis-Specific CD8 T-Cells in Patients with Active Tuberculosis and in Individuals with Latent Infection

2009

CD8 T-cells contribute to control of Mycobacterium tuberculosis infection, but little is known about the quality of the CD8 T-cell response in subjects with latent infection and in patients with active tuberculosis disease. CD8 T-cells recognizing epitopes from 6 different proteins of Mycobacterium tuberculosis were detected by tetramer staining. Intracellular cytokines staining for specific production of IFN-gamma and IL-2 was performed, complemented by phenotyping of memory markers on antigen-specific CD8 T-cells. The ex-vivo frequencies of tetramer-specific CD8 T-cells in tuberculous patients before therapy were lower than in subjects with latent infection, but increased at four months a…

MaleEpitopes T-Lymphocytelcsh:MedicineCD8-Positive T-LymphocytesEpitopeDiagnostic RadiologyInfectious Diseases/Bacterial InfectionsSpectrum Analysis TechniquesCellular typesCytotoxic T celllcsh:ScienceImage Cytometryeducation.field_of_studyMultidisciplinarybiologyRadiology and ImagingImmune cellsInfection ImagingMiddle AgedFlow CytometryActinobacteriaPhenotypeSpectrophotometryCytokinesWhite blood cellsFemaleCytophotometryResearch Articlemedicine.drugInterleukin 2Cell biologyBlood cellsTuberculosisImaging TechniquesImmunologyPopulationT cellsCytotoxic T cellsResearch and Analysis MethodsMycobacterium tuberculosisDiagnostic MedicineImmunology/Immunity to InfectionsHLA-A2 AntigenmedicineHumansTuberculosiseducationMedicine and health sciencesHLA-A AntigensBacteriaFluorimetrylcsh:ROrganismsBiology and Life SciencesMycobacterium tuberculosisbiology.organism_classificationmedicine.diseaseVirologyRetractionAnimal cellsImmunology/Immune ResponseImmunologyMycobacterium tuberculosis CD8 T cells Tuberculosis Latent Infectionlcsh:QCD8MycobacteriumPLoS ONE
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Migration of CD11b+ accessory cells during murine lung regeneration

2013

In many mammalian species, the removal of one lung leads to growth of the remaining lung to near-baseline levels. In studying post-pneumonectomy mice, we used morphometric measures to demonstrate neoalveolarization within 21 days of pneumonectomy. Of note, the detailed histology during this period demonstrated no significant pulmonary inflammation. To identify occult blood-borne cells, we used a parabiotic model (wild-type/GFP) of post-pneumonectomy lung growth. Flow cytometry of post-pneumonectomy lung digests demonstrated a rapid increase in the number of cells expressing the hematopoietic membrane molecule CD11b; 64.5% of the entire GFP(+) population were CD11b(+). Fluorescence microscop…

MaleLeukocyte migrationmedicine.medical_treatmentPopulationBiologyArticleGreen fluorescent proteinFlow cytometryMice03 medical and health sciencesPneumonectomy0302 clinical medicineCell MovementLeukocytesmedicineAnimalsRegenerationPneumonectomyeducationLung030304 developmental biologyMedicine(all)0303 health scienceseducation.field_of_studyBlood CellsCD11b AntigenLungmedicine.diagnostic_testHistologyGeneral MedicineCell BiologyMolecular biology3. Good healthMice Inbred C57BLHaematopoiesisPhenotypemedicine.anatomical_structureCD18 Antigens030220 oncology & carcinogenesisImmunologyTranscriptomeBronchoalveolar Lavage FluidDevelopmental BiologyStem Cell Research
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