Search results for "BoP"

showing 10 items of 284 documents

ProC Global: the first functional screening assay for the complete protein C pathway.

1997

Abstract In clinical practice, venous thromboembolic complications are much more frequent than bleeding disorders. In fact, disturbances within the protein C pathway due to coagulation factor V (FV) Leiden mutation and deficiency of protein C or protein S are the most frequent abnormalities in hereditary thrombophilia. Furthermore, acquired dysfunctions of the protein C system may predispose the single individual to an increased thrombotic risk. A routine-suited screening assay that would allow the monitoring of the proper interplay of factors in the protein C pathway could add an important factor to the basic coagulation profile. This consists of the prothrombin time and of the activated p…

Clinical BiochemistryBlood DonorsSensitivity and SpecificityProtein SProtein SProtein C deficiencyReference ValuesMedicineHumansMass ScreeningProtein S deficiencyProthrombin timemedicine.diagnostic_testbiologybusiness.industryBiochemistry (medical)Factor VFactor VProtein C DeficiencyReproducibility of ResultsThrombosisBlood Coagulation Disordersmedicine.diseaseImmunologyMutationbiology.proteinCancer researchPartial Thromboplastin TimeDisease SusceptibilityReagent Kits DiagnosticActivated protein C resistancebusinessProtein CPartial thromboplastin timemedicine.drugProtein CClinical chemistry
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Effects of pasireotide treatment on coagulative profile: a prospective study in patients with Cushing's disease.

2018

Introduction: Cushing’s disease (CD) is characterized by procoagulative profile. Treatment with cortisol-reducing medications might normalize the coagulation impairment potentially eliminating the risk of thromboembolic complications. Aim: The aim of this prospective study is to evaluate the effectiveness of 6–12 months of treatment with pasireotide (Signifor®, Novartis) 600 µg twice daily on coagulative factors in 21 patients (16 females, mean age 46 ± 12.2 years) with CD. Biochemical, hormonal (urinary free cortisol, UFC; late night salivary cortisol, LNSC; ACTH) and coagulative parameters as Protrombin time (PT), aPTT, factors VIII, IX and XI, antithrombin III, protein C, protein S, fibr…

Cortisol secretionAdultBlood GlucoseMalemedicine.medical_specialtymedicine.drug_classEndocrinology Diabetes and Metabolism030209 endocrinology & metabolismGastroenterologySettore MED/13 - Endocrinologia03 medical and health scienceschemistry.chemical_compound0302 clinical medicineEndocrinologyInternal medicinemedicineHumansProspective StudiesProspective cohort studyThromboprophylaxisPituitary ACTH HypersecretionBlood CoagulationClotting factorCoagulationbusiness.industryAnticoagulantCoagulation; Cushing’s disease; Pasireotide; Pituitary; Thromboprophylaxis; Endocrinology Diabetes and Metabolism; EndocrinologyCushing's diseaseCushing’s diseaseMiddle Agedmedicine.diseasePasireotidePasireotideDiabetes and MetabolismCoagulative necrosisTreatment OutcomechemistryPituitary030220 oncology & carcinogenesisConcomitantThromboprophylaxiFemaleBlood Coagulation TestsbusinessSomatostatinEndocrine
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Matrix solid-phase dispersion extraction procedure for multiresidue pesticide analysis in oranges.

1996

A multiresidue extraction method based on matrix solid-phase dispersion (MSPD) is optimized for the extraction and gas chromatographic screening of eighteen insecticides (aldrin, carbophenothion, captafol, chlorpyriphos, chlorfenvinphos, diazinon, dicofol, alpha-endosulfan, beta-endosulfan, ethion, fenitrothion, folpet, methidathion, malathion, methyl-azinphos, methyl-parathion, phosmet, and tetradifon) from oranges. After optimization of different parameters, such as type of solid phase used and the amount of solid phase or eluent, recoveries ranged from 67 to 102% with relative standard deviations ranging from 2 to 10%. The limits of detection, calculated as 3 times the baseline noise ran…

Detection limitCitrusChromatographyChromatography GasPesticide residueMicrochemistryOrganic ChemistryAnalytical chemistryPesticide ResiduesGeneral MedicinePhosmetMethidathionAcetatesEthionBiochemistrySensitivity and SpecificityAnalytical ChemistryTetradifonchemistry.chemical_compoundchemistryCarbophenothionSolventsAldrinJournal of chromatography. A
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Paradoxical embolism after a femoral fracture

1998

The foramen ovale is anatomically open in 25 % of individuals, but functionally closed by the higher pressure in the left antrum. Right-to-left shunt and subsequent paradoxical embolism may occur when pressure in the left antrum rises, for example, as a result of pulmonary embolism. In the present case we demonstrate a patient who presented 20 days after osteosynthetic treatment of a femoral fracture with word-finding deficits. Cerebral MRT revealed a fresh ischemic insult. Duplex ultrasound of the legs showed a fresh thrombosis of the superficial femoral vein and scintigraphy of the lungs detected pulmonary embolism. Transesophageal contrast echocardiography trapped a hemodynamically spont…

Diagnostic Imagingmedicine.medical_specialtyDeep veinIschemiaFemoral veinHeart Septal Defects AtrialFracture Fixation InternalPostoperative ComplicationsParadoxical embolismRisk FactorsmedicineHumansOrthopedics and Sports MedicineAgedHip Fracturesbusiness.industryFemoral fractureIntracranial Embolism and ThrombosisThrombophlebitismedicine.diseaseThrombosisPulmonary embolismmedicine.anatomical_structureEmbolismEmergency MedicineFemaleSurgeryRadiologyPulmonary EmbolismbusinessEmbolism ParadoxicalDer Unfallchirurg
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Rat fetuin: distribution of protein and mRNA in embryonic and neonatal rat tissues

1998

Fetuin is a serum protein widely distributed in the animal kingdom and found in all mammalian species so far investigated. It is mainly a fetal protein, in the sense that the highest concentrations are found in serum and body fluids of embryos and fetuses. In order to elucidate possible biological functions of fetuin, we have studied its synthesis and distribution during the prenatal development of the rat with immunohistochemistry and in situ hybridization. We have isolated fetuin from rat serum and produced an antibody against this protein. In situ hybridization was performed using a 375-nucleotides-long digoxigenin-labeled riboprobe. Fetuin was unevenly distributed in all organ systems d…

Embryologymedicine.medical_specialtyCellular differentiationIn situ hybridizationBiologyGastrointestinal epitheliumImmunoenzyme TechniquesMiceInternal medicinemedicineAnimalsTissue DistributionRNA MessengerIn Situ HybridizationFetal proteinFetusRiboprobeCell BiologyBlotting NorthernEmbryo MammalianFetuinRatsCell biologyEndocrinologyAnimals NewbornAntibody FormationImmunohistochemistryFemaleRabbitsalpha-FetoproteinsAnatomyDevelopmental BiologyAnatomy and Embryology
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Graphene coating obtained in a cold-wall CVD process on the Co-Cr Alloy (L-605) for medical applications

2021

Graphene coating on the cobalt-chromium alloy was optimized and successfully carried out by a cold-wall chemical vapor deposition (CW-CVD) method. A uniform layer of graphene for a large area of the Co-Cr alloy (discs of 10 mm diameter) was confirmed by Raman mapping coated area and analyzing specific G and 2D bands

ErythrocytesMicroscopeScanning electron microscope02 engineering and technologyChemical vapor deposition01 natural scienceslaw.inventionlcsh:ChemistryMiceCoated Materials BiocompatibleCoatinglawMaterials TestingComposite materiallcsh:QH301-705.5SpectroscopySettore CHIM/02 - Chimica Fisicagraphene coating ; biocompatibility ; cobalt chromium alloy ; cold wall chemical vapor deposition methodGeneral Medicine021001 nanoscience & nanotechnologyMicrostructureBlood Coagulation FactorsComputer Science ApplicationsGraphitePartial Thromboplastin TimeBiocompatibility0210 nano-technologyLayer (electronics)Blood PlateletsMaterials scienceCell SurvivalSurface PropertiesPrimary Cell Cultureengineering.material010402 general chemistryCobalt-chromium alloyGraphene coatingCold-wall chemical vapor deposition methodArticleCatalysisInorganic ChemistryAnimalsHumansPhysical and Theoretical ChemistryMolecular BiologyGrapheneOrganic Chemistrytechnology industry and agricultureNanoindentationPlatelet Activation0104 chemical scienceslcsh:Biology (General)lcsh:QD1-999NIH 3T3 CellsengineeringChromium AlloysVolatilization
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Glucocorticoid receptor (DlGR1) is expressed in pre-larval and larval stages of the teleost fish Dicentrarchus labrax.

2008

Glucocorticoid hormone receptors (GR), members of the nuclear hormone receptor superfamily, are ligand-dependent transcription factors expressed in various tissues by binding to specific DNA sequences. Since glucocorticoids have a role in maintaining the homeostatic status in fish, we previously cloned and sequenced a GR (DlGR1) of adult Dicentrarchus labrax; we also showed mRNA expression (in situ hybridization) and tissue immunohistochemical localization of DlGR1 in several organs. This work has now been extended to the examination of the expression, tissue distribution, and cytolocalization of DlGR1 in larval developmental stages by similar methods to those used for the adult organs. The…

Fish ProteinsHistologySettore BIO/05 - ZoologiaGene ExpressionIn situ hybridizationGlucocorticoid receptorBiologyArticlePathology and Forensic MedicineGlucocorticoid receptorReceptors GlucocorticoidComplementary DNAGene expressionAnimalsDicentrarchus labrax (Teleostei)Larval development . Glucocorticoid receptor . In situ hybridization . Immunohistochemistry . Dicentrarchus labrax (TeleosteiReceptorPeptide sequenceRiboprobeCell BiologyMolecular biologyImmunohistochemistryNuclear receptorLarvaLarval developmentBassIn situ hybridizationCell and tissue research
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Expression of a glucocorticoid receptor (DlGR1) in several tissues of the teleost fish Dicentrarchus labrax

2008

Since glucocorticoids have a role in maintaining the homeostatic status in fish, in the present paper mRNA expression (in situ hybridization) and tissue immunohistochemical localization of a glucocorticoid receptor (DlGR1) in several Dicentrarchus labrax organs are reported. Riboprobe and specific antibodies were prepared by using the DlGR1 that has been previously cloned and sequenced from peritoneal cavity leukocytes. Both mRNA and receptor were identified in head kidney, spleen, gills, intestine, heart and liver tissues. The functional roles of DlGR1 localization are discussed.

Fish Proteinsmedicine.medical_specialtyBlotting WesternGene ExpressionSpleenIn situ hybridizationBiologyPeritoneal cavityReceptors GlucocorticoidGlucocorticoid receptorInternal medicinemedicineAnimalsRNA MessengerReceptorIn Situ HybridizationRiboprobeCell BiologyGeneral Medicinebiology.organism_classificationImmunohistochemistryMolecular biologymedicine.anatomical_structureEndocrinologyImmunohistochemistryBassDicentrarchusDevelopmental BiologyTissue and Cell
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Comparison of the Enhancement Pattern of Hepatic Hemangioma on Magnetic Resonance Imaging Performed With Gd-EOB-DTPA Versus Gd-BOPTA

2019

Abstract Purpose To compare magnetic resonance imaging (MRI) findings with gadoxetic acid and gadobenate dimeglumine for the diagnosis of hepatic hemangiomas. Materials and Methods In this retrospective study, we included 26 hemangiomas (mean size was 14 mm ± 10 mm) in 19 patients (mean age 60 ± 14 years) scanned with both gadobenate dimeglumine MRI and gadoxetic acid MRI. For each patient, we collected multiple lesion variables including location, number, size and enhancement pattern on arterial, portal venous, 3-minute and hepatobiliary phases with both gadoxetic acid and gadobenate dimeglumine. The enhancement pattern with the two contrast agents was then compared. Results The typical en…

Gadolinium DTPAMaleHepatic HemangiomaGadoxetic acidGd boptaContrast MediaGd-EOB-DTPAEnhancement patternliver030218 nuclear medicine & medical imaging03 medical and health sciencesmagnetic resonance imaging.Meglumine0302 clinical medicineImage Interpretation Computer-AssistedOrganometallic CompoundsmedicineHumansRadiology Nuclear Medicine and imagingRetrospective Studiesmedicine.diagnostic_testbusiness.industryLiver NeoplasmsMagnetic resonance imagingMiddle AgedImage EnhancementMagnetic Resonance ImagingHyperintensitygadoxetic acid030220 oncology & carcinogenesisFemaleNuclear medicinebusinessHemangiomaArterial phasemedicine.druggadobenate dimeglumine
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Screening for multiple hereditary hypercoagulability factors using the amplification refractory mutation system

2003

Many hereditary factors have been implicated in the development of arterial and/or venous thromboembolic diseases. A number of these risk factors can be identified by the amplification refractory mutation system (ARMS). However, the underlying technical conditions for performing ARMS are highly variable, and depend on which risk factors are being analyzed. We have now developed a novel ARMS-based system to simultaneously screen for multiple hypercoagulability factors under identical PCR conditions. This can greatly simplify the process of screening for hereditary hypercoagulability.

GeneticsBase SequenceGenetic Carrier ScreeningHomozygoteGenetic Carrier ScreeningSingle-strand conformation polymorphismBlood ProteinsHematologyBlood Coagulation DisordersBiologymedicine.diseaseThrombophiliaBioinformaticsPolymerase Chain ReactionThrombosisBlood Coagulation FactorsRefractoryMutation (genetic algorithm)medicineCoagulopathyHumansMass ScreeningRisk factorDNA PrimersThrombosis Research
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