Search results for "Botrytis"

Characterization of a new, nonpathogenic mutant of Botrytis cinerea with impaired plant colonization capacity.

2006

International audience; Botrytis cinerea is a necrotrophic pathogen that attacks more than 200 plant species.Here, the nonpathogenic mutant A336, obtained via insertional mutagenesis, was characterized.Mutant A336 was nonpathogenic on leaves and fruits, on intact and wounded tissue, while still able to penetrate the host plant. It grew normally in vitro on rich media but its conidiation pattern was altered. The mutant did not produce oxalic acid and exhibited a modified regulation of the production of some secreted proteins (acid protease 1 and endopolygalacturonase 1). Culture filtrates of the mutant triggered an important oxidative burst in grapevine ( Vitis vinifera ) suspension cells, a…

Isolation frequency and efficiency of mycoparasitic Pythium species in the West Mediterranean region of Turkey

2021

Abstract A total of 132 mycoparasitic Pythium isolates were obtained from the soil samples taken from fruit orchards, vegetable fields, carnation greenhouses, sugarbeet fields and cereal fields in the West Mediterranean region of Turkey. The Oomycetes were isolated by using; surface soil dilution plate, sclerotia bait and precolonised plate methods. Isolates were identified as P. acanthophoron, P. amasculinum, P. lycopersicum, P. oligandrum, P. periplocum and P. paroecandrum, according to their morphological and molecular characteristics. The most commonly isolated mycoparasitic species were P. amasculinum and P. lycopersicum. The majority of the mycoparasites came from soil samples taken f…

Priming for JA-dependent defenses using hexanoic acid is an effective mechanism to protect Arabidopsis against B. cinerea

2011

Abstract Soil drench treatments with hexanoic acid can effectively protect Arabidopsis plants against Botrytis cinerea through a mechanism based on a stronger and faster accumulation of JA-dependent defenses. Plants impaired in ethylene, salicylic acid, abscisic acid or glutathion pathways showed intact protection by hexanoic acid upon B. cinerea infection. Accordingly, no significant changes in the SA marker gene PR-1 in either the SA or ABA hormone balance were observed in the infected and treated plants. In contrast, the JA signaling pathway showed dramatic changes after hexanoic acid treatment, mainly when the pathogen was present. The impaired JA mutants, jin1-2 and jar1 , were unable …

Can the grey mould disease of the grape-vine be controlled by yeast?

2000

Botrytis cinerea has been found to be highly pathogenic to ‘Chardonnay’ and ‘Pinot noir’ cultivars of the grape-vine producing the characteristic grey mould symptoms within 7 days of inoculation to the vitro-plants. The yeast Pichia anomala (strain FY-102), isolated from apple skin, was found to be antagonistic to B. cinerea as it completely inhibited the appearance of the grey mould symptoms when grown together. The yeast was responsible for morphological changes such as coagulation and leakage of the cytoplasm of B. cinerea. The pathogen, when applied together with P. anomala, failed to bring about the grey mould symptoms on the grape-vine, suggesting that the yeast could control the expr…

Resistance to Nano-Based Antifungals Is Mediated by Biomolecule Coronas.

2018

Fungal infections are a growing global health and agricultural threat, and current chemical antifungals may induce various side-effects. Thus, nanoparticles are investigated as potential novel antifungals. We report that nanoparticles' antifungal activity strongly depends on their binding to fungal spores, focusing on the clinically important fungal pathogen Aspergillus fumigatus as well as common plant pathogens, such as Botrytis cinerea. We show that nanoparticle-spore complex formation was enhanced by the small nanoparticle size rather than the material, shape or charge, and could not be prevented by steric surface modifications. Fungal resistance to metal-based nanoparticles, such as Zn…

The vacuole membrane (tonoplast) from the meristematic cells of Brassica oleracea var. Botrytis contains major intrinsic proteins related to tips: A …

1995

An untargeted global metabolomic analysis reveals the biochemical changes underlying basal resistance and priming in Solanum lycopersicum, and identi…

2015

n this study, we have used untargeted global metabolomic analysis to determine and compare the chemi-cal nature of the metabolites altered during the infection of tomato plants (cv. Ailsa Craig) with Botry-tis cinerea (Bot)orPseudomonas syringae pv. tomato DC3000 (Pst), pathogens that have different invasionmechanisms and lifestyles. We also obtained the metabolome of tomato plants primed using the naturalresistance inducer hexanoic acid and then infected with these pathogens. By contrasting the metabolomicprofiles of infected, primed, and primed + infected plants, we determined not only the processes or compo-nents related directly to plant defense responses, but also inferred the metabolic…

Validation of a predictive model for the growth of Botrytis cinerea and Penicillium expansum on grape berries.

2010

The objective of this study was to develop and to validate a model for predicting the combined effect of temperature and a(w) on the radial growth rate, mu, of Botrytis cinerea and Penicillium expansum on grape berries. The proposed strategy was based on the gamma-concept developed previously [Zwietering, M.H., Wijtzes, T., de Wit, J.C., van't Riet, K. 1992. A decision support system for prediction of the microbial spoilage in foods. Journal of Food Protection. 12, 973-979]: mu=mu(opt).gamma(T).gamma(a(w)), where the gamma functions were cardinal models with inflection (CMI), mu(opt) the radial growth rate on grape berries. Firstly, the cardinal temperatures and a(w)'s were estimated indepe…

Identification of a copper chaperone from tomato fruits infected with Botrytis cinerea by differential display

2003

Differential display was used to isolate tomato genes responding to fungal infection. Here we describe the isolation and characterization of a gene that is down-regulated in tomato fruits infected with the phytopathogen Botrytis cinerea. The cDNA identified encodes a protein that shares sequence similarity to the amino terminal region of CCH, a copper chaperone from Arabidopsis thaliana, that participates in intracellular copper homeostasis by delivering Cu to the secretory pathway. The fact that this newly characterized tomato gene, referred to as LeCCH (Lycopersicon esculentum copper chaperone), be differentially expressed after fungal infection, suggests an interesting relationship betwe…

Characterisation of the yeast Pichia membranifaciens and its possible use in the biological control of Botrytis cinerea, causing the grey mould disea…

2001

Pichia membranifaciens strain FY-101, isolated from grape skins, was found to be antagonistic to Botrytis cinerea, the causal organism of the grey mould disease of the grapevine. When grown together on solid as well as liquid media, the yeast brings about the inhibition of this parasitic fungus, coagulation and leakage of its cytoplasm, and suppression of its ability to produce the characteristic grey mould symptoms on the grapevine plantlets. In vitro experiments confirm that this yeast can be used as a biological control organism against B. cinerea. An account of the molecular characterisation of P. membranifaciens (complete sequence of the ITS region of its ribosomal DNA, GenBank accessi…