Search results for "CA2+"
showing 10 items of 101 documents
Polymorphonuclear leukocyte membrane fluidity and cytosolic Ca2+ concentration in subjects with vascular atherosclerotic disease subdivided according…
2006
An abnormal activation state of polymorphonuclear leukocytes (PMN) plays a key role in organ injury induced by vascular atherosclerotic disease (VAD) and diabetes mellitus (DM). PMN membrane fluidity and cytosolic Ca2+ content can be considered markers of PMN activation. In this research we evaluated the PMN membrane fluidity and cytosolic Ca2+ content in VAD subjects with and without type 2 DM and examined the association between these parameters and the mono- or polyvascular localization. We enrolled 155 VAD subjects, including 92 non-diabetic (group A: mean age 63.6 +/- 9.2 years) and 63 diabetic patients (group B: mean age 65.4 +/- 7.8 years). Among group A 63 patients had monovascular …
Fluidity and cytosolic Ca2+ concentration of circulating polymorphonuclear leukocytes at baseline in some chronic and acute clinical conditions: revi…
2016
Abstract. Objective: In this mini-review we describe the behavior of polymorphonuclear leukocyte (PMN) membrane fluidity and of PMN cytosolic Ca2+ concentration in some chronic and acute clinical conditions. Methods: PMN membrane fluidity was evaluated employing the fluorescent probe Fura-2AM, and PMN cytosolic Ca2+ concentration was evaluated using the fluorescent probe TMA-DPH. Results: From the determination of these two parameters investigated on resting PMNs, an almost constant increase in PMN cytosolic Ca2+ concentration in chronic clinical conditions, such as vascular atherosclerotic disease with and without diabetes mellitus, essential hypertension, chronic kidney disease, and diabe…
Synthesis of substituted carbamo(dithioperoxo)thioates as potential BCA2-inhibitory anticancer agents
2015
A new, simple, one-step synthetic route to carbamo(dithioperoxo)thioates from commercially available starting materials is described. The key step of this new synthetic approach involves the tetrabromomethane-promoted reaction between secondary amines, carbon disulfide and alkyl thiols under basic conditions at room temperature. New compounds from this series selected for anticancer screening showed selective sub-micromolar activity within BCA2-expressing human breast cancer cell lines.
SULT1A1 gene deletion in BRCA2-associated male breast cancer: a link between genes and environmental exposures?
2013
SULT1A1, a member of sulfotransferase superfamily, is a drug and hormone metabolizing enzyme involved in the metabolism of a variety of potential mammary carcinogens of endogenous and exogenous origin. Interestingly, the metabolic activity of SULT1A1 can be affected by varia- tions in gene copy number. Male Breast Cancer (MBC) is a rare disease and less investigated disease compared to female BC (FBC). As in FBC, the concurrent effects of genetic risk factors, particularly BRCA2 mutations, increased exposure to estrogens and environmental carcinogens play a relevant role in MBC. By quantitative real-time PCR with TaqMan probes, we investigated the presence of SULT1A1 gene copy number variat…
Cáncer hereditario: Importancia y necesidad de su detección
2013
A pesar de que tan solo el 1% de los casos de cáncer se pueden considerar de tipo hereditario, en la actualidad se han descrito más de 200 síndromes caracterizados por la agregación familiar de distintos tipos de tumores. La identificación a lo largo del siglo XX de muchos de los genes responsables de estos síndromes ha permitido un gran avance en el manejo de estas familias, a la hora de identificar o descartar los individuos a riesgo y establecer unas medidas preventivas específicas. Sin embargo, existe todavía un alto porcentaje de los casos hereditarios en los que la causa de la susceptibilidad es desconocida, siendo la búsqueda de nuevos genes mediante las nuevas tecnologías una de la…
Pellets based on polyuronates: Relationship between gelation and release properties
2017
International audience; Three polyuronates: amidated low methoxyl pectin (ALMP), low methoxyl pectin (LMP) and polygalacturonic acid (PGA) were used to encapsulate rutin in pellets, and they were characterized by different techniques (macroscopic properties, Calcium and rutin release). The ability of the three polyuronates to bind calcium ions and the viscoelastic properties of gels were performed to relate the properties of the pellets to the gel structures. The pellets size, the water content, the water uptake, the release of calcium and rutin varied depending on the polyuronate used. The pellets size of ALMP were smaller than LMP and PGA with a lower water content, but this matrix was mo…
Light-dependent CK2-mediated phosphorylation of centrins regulates complex formation with visual G-protein.
2008
AbstractCentrins are Ca2+-binding EF-hand proteins. All four known centrin isoforms are expressed in the ciliary apparatus of photoreceptor cells. Cen1p and Cen2p bind to the visual G-protein transducin in a strictly Ca2+-dependent way, which is thought to regulate light driven movements of transducin between photoreceptor cell compartments. These relatively slow motile processes represent a novel paradigm in light adaptation of photoreceptor cells.Here we validated specific phosphorylation as a novel regulator of centrins in photoreceptors. Centrins were differentially phosphorylated during photoreceptor dark adaptation. Inhibitor treatments revealed protein kinase CK2 as the major protein…
Ekstracelulārās kalcija koncentrācijas ietekme uz krūts vēža šūnu līnijas MCF-7 migrāciju un invāziju
2018
Visbiežākais ar krūts vēzi saistītais nāves iemesls ir metastāzes citos orgānos. Lai metastāzes veidotos, šūnām ir jābūt spējīgām migrēt un invadēt. Tomēr pētījumu rezultāti par ekstracelulārā Ca2+ ietekmi uz krūts vēža šūnu spēju migrēt un invadēt ir pretrunīgi. Darba mērķis bija noteikt ekstracelulārā Ca2+ koncentrācijas ietekmi uz krūts vēža šūnu līnijas MCF-7 migrāciju un invāziju. Šūnas tika kultivētas barotnēs ar dažādu Ca2+ koncentrāciju. Tika konstatēts, ka palielināta ekstracelulārā Ca2+ koncentrācija barotnē samazina MCF-7 šūnu spēju migrēt un invadēt, bet neietekmē populācijas dubultošanās laiku. Šūnās, kas kultivētas barotnēs ar palielinātu Ca2+ koncentrāciju, ciešo starpšūnu ko…
The pharmacological rationale for combining muscarinic receptor antagonists and beta-adrenoceptor agonists in the treatment of airway and bladder dis…
2014
Highlights • Muscarinic receptors increase smooth muscle tone in airways and urinary bladder. • β-Adrenoceptors relax smooth muscle tone and oppose muscarinic contraction. • Opposition involves transmitter release, signal transduction and receptor expression. • This supports the combined use of muscarinic antagonists and β-adrenoceptor agonists.
Intron variants of the p53 gene are associated with increased risk for ovarian cancer but not in carriers of BRCA1 or BRCA2 germline mutations
1999
Two biallelic polymorphisms in introns 3 and 6 of the p53 gene were analysed for a possible risk-modifying effect for ovarian cancer. Germline DNA was genotyped from 310 German Caucasian ovarian cancer patients and 364 healthy controls. We also typed 124 affected and 276 unaffected female carriers with known deleterious BRCA1 or BRCA2 germline mutation from high-risk breast-ovarian cancer families. Genotyping was based on PCR and high-resolution gel electrophoresis. German ovarian cancer patients who carried the rare allele of the MspI restriction fragment length polymorphism (RELP) in intron 6 were found to have an overall 1.93-fold increased risk (95% confidence internal (CI) 1.27–2.91) w…