Search results for "CEF"
showing 10 items of 318 documents
Cocirculation of Hajj and non-Hajj strains among serogroup W meningococci in Italy, 2000 to 2016
2019
In Italy, B and C are the predominant serogroups among meningococci causing invasive diseases. Nevertheless, in the period from 2013 to 2016, an increase in serogroup W Neisseria meningitidis (MenW) was observed. This study intends to define the main characteristics of 63 MenW isolates responsible of invasive meningococcal disease (IMD) in Italy from 2000 to 2016. We performed whole genome sequencing on bacterial isolates or single gene sequencing on culture-negative samples to evaluate molecular heterogeneity. Our main finding was the cocirculation of the Hajj and the South American sublineages belonging to MenW/clonal complex (cc)11, which gradually surpassed the MenW/cc22 in Italy. All M…
Validated HPLC-UV detection method for the simultaneous determination of ceftolozane and tazobactam in human plasma
2018
Aim: A simple, rapid, economical and sensitive HPLC-UV method was developed for the simultaneous quantification of ceftolozane and tazobactam in plasma samples. Methodology: After deproteinization followed by a liquid–liquid back-extraction, the compounds were separated on a C18 column (150 mm × 4.6 mm, 5 μm) with UV-visible detection at 220 nm. The mobile phase consisted of acetonitrile and potassium dihydrogenphosphate buffer at pH 3.0 (8:92, v/v), delivered isocratically at a flow rate of 1.0 ml/min and at a column oven temperature of 30°C. Cefepime was used as an internal standard. Results: Linearity was achieved in the concentration range of 0.50–100.00 μg/ml for ceftolozane and 0.25–…
Preservation of Multiple Mammalian Tissues to Maximize Science Return from Ground Based and Spaceflight Experiments.
2016
Background Even with recent scientific advancements, challenges posed by limited resources and capabilities at the time of sample dissection continue to limit the collection of high quality tissues from experiments that can be conducted only infrequently and at high cost, such as in space. The resources and time it takes to harvest tissues post-euthanasia, and the methods and duration of long duration storage, potentially have negative impacts on sample quantity and quality, thereby limiting the scientific outcome that can be achieved. Objectives The goals of this study were to optimize methods for both sample recovery and science return from rodent experiments, with possible relevance to b…
Human Mesenchymal Stem Cells Prevent Neurological Complications of Radiotherapy
2019
Radiotherapy is a highly effective tool for the treatment of brain cancer. However, radiation also causes detrimental effects in the healthy tissue, leading to neurocognitive sequelae that compromise the quality of life of brain cancer patients. Despite the recognition of this serious complication, no satisfactory solutions exist at present. Here we investigated the effects of intranasal administration of human mesenchymal stem cells (hMSCs) as a neuroprotective strategy for cranial radiation in mice. Our results demonstrated that intranasally delivered hMSCs promote radiation-induced brain injury repair, improving neurological function. This intervention confers protection against inflamma…
A case of infective colitis due to Yersinia enterocolitica complicated by microliver abscesses mimicking multiple liver occult metastases: a case rep…
2021
Abstract Background We report an unusual case of infective colitis by Yersinia enterocolitica complicated by microliver abscesses mimicking multiple liver metastases in a 79 yr old female without any risk factors for bacteriaemia by this pathogen. Case presentation The patient was admitted to the Internal Medicine with Stroke Care ward of University Policlinico “P. Giaccone” in Palermo because of the appearance of diarrhoea. After the antimicrobial treatment for infective colitis, the clinicians observed a persistently increased white blood cells (WBC) count and multiple hepatic lesions; after having excluded any neoplastic disease and inflammatory bowel disease (IBD), blood cultures positi…
Word derivational knowledge and writing proficiency: How do they link?
2016
Abstract Although word derivational (WD) knowledge, i.e., how new words are formed from existing words with help of derivational affixes, is considered important for learners of second or foreign languages (L2), there is still no clear answer as to what aspects comprise the construct of L2 English word derivational knowledge and how it develops. The present study adds to our knowledge on how the ability to derive English words develops among L2 English learners. More specifically, it sheds light on how word derivational knowledge relates to communicatively defined Common European Framework of Reference (CEFR) language proficiency levels regarding learners' writing skills. In the study, 117 …
Feedback practices in language classes in Finnish general upper secondary schools
2020
As feedback and formative assessment have a substantial effect on learning, the aim with this paper is to report on a study of the perceptions of Finnish general upper secondary school students of feedback in Swedish and English classes, and to compare how the perceptions differ at language proficiency (CEFR) levels. The data were collected by using a survey and were analysed quantitatively. The results show that several differences occur in Swedish: students with higher proficiency levels find feedback more useful, feel that they receive feedback from teachers, and are more willing to correct their own mistakes. There were no differences in perceptions according to language proficiency lev…
Papel de los neuroesteroides en la modulación de la vía glutamato-óxido nítrico-GMP cíclico. Alteraciones en hiperamonemia crónica. Papel en las alte…
2013
En el cerebelo de ratas hiperamonémicas existe un aumento del tono GABAérgico que está implicado en el deterioro de la función de la vía Glu-NO-GMPc y de las capacidades de aprendizaje y memoria moduladas por esta vía. Al aumento del tono GABAérgico contribuyen: • un aumento de la concentración extracelular de GABA en cerebelo. • un aumento de la cantidad de algunas subunidades de receptores GABAA. • un aumento de la concentración de neuroesteroides que modulan la activación de receptores GABAA. Los neuroesteroides pueden modular diversos receptores incluyendo el receptor GABAA, el NMDA y los receptores sigma. Esta tesis parte de las hipótesis de que: • algunos neuroesteroides modulan, a co…
Estudio de alteraciones neurofisiológicas durante el sueño en pacientes con encefalopatía hepática mínima
2021
Una proporción importante de los pacientes con cirrosis y otras enfermedades hepáticas van a desarrollar una afectación del sistema nervioso central en forma de encefalopatía hepática. En fases iniciales, la clínica puede pasar desapercibida, estadio que da lugar al concepto de encefalopatía hepática mínima (EHM), que es habitualmente diagnosticada mediante test neuropsicológicos. Las alteraciones del sueño pueden ser las primeras manifestaciones neurológicas de la EHM y se asocian con una disminución de la calidad de vida. Existe un interés en la obtención de biomarcadores que permitan identificar a estos pacientes para su tratamiento precoz. La hipótesis del estudio parte de si la polisom…
Efectos de la hiperamonemia y la encefalopatía hepática sobre la conectividad neuronal en cerebro de pacientes cirróticos y modelos animales. Implica…
2018
La encefalopatía hepática (HE) es un complejo síndrome neuropsiquiátrico producido por la alteración en la función del sistema nervioso central asociada a la cirrosis hepática. Entre un 33-55% de los pacientes cirróticos sin síntomas clínicos evidentes de EH padecen encefalopatía hepática mínima (EHM) con deterioro cognitivo leve que empeora su calidad y esperanza de vida. Los pacientes cirróticos con EHM presentan alteraciones en la generación del potencial MMN (Mismatch Negativity), concretamente una disminución del área bajo la curva del potencial, que se correlaciona con los déficits de atención. En esta tesis hemos demostrado que las ratas con hiperamonemia crónica reproducen las alter…