Search results for "CELLULAR"

Corrigendum to “Anticonvulsant effects of carbenoxolone in genetically epilepsy prone rats (GEPRs)”[Neuropharmacology 47 (2004) 1205-1216]

2009

Adenylate storage, metabolism and utilization in coelomic cells of the polychaeteNereis virens (Annelida, polychaeta)

1996

Eleocytes are specialized coelomic cells in nereid annelids which assume a central role during germ cell development. They may contain extremely high concentrations of both adenosine monophosphate (AMP) and adenosine diphosphate (ADP) (each >10 μmol/ml of cell vol.), whereas the adenosine triphosphate (ATP) content is comparatively low (0.8 μmol/ml cell vol.).31P nuclear magnetic, resonance (NMR) studies of living eleocytes suggest the compartmentalization of both AMP and ADP in the large acidic vacuole characteristic for this cell type. Eleocytes are thus capable of storing high concentrations of ADP and AMP without inhibiting energy metabolism, by sequestering these compounds in a separat…

Thermische Denaturierung von Kaltblüter-Enzymproteinen

1960

The temperature, which leads to 50% reduction of catalytic activity by heat denaturation, has been determined for 8 different enzymes from cod muscle, as being in the range between 30 and 52°C. Therefore, there are no indications of a generally different heat resistance of enzymes from cold-blooded animals as compared with those from warm-blooded animals. The same conclusion is derived from calculations ofQ10-values, measured between +37 and − 37°C for cathepsin and glycylglycine dipeptidase.

Untersuchung von Adenosintriphosphorsäure (ATP), Adenosindiphosphorsäure (ADP), Adenosinmonophosphorsäure (AMP) und Inosinsäure (IMP) in ruhenden und…

1954

A method for the estimation of muscle nucleotides is described. The separation of nucleotides has been accomplished by paper ionophoresis at high voltages, as well as by paper chromatography. Even little changes in the nucleotide content such as the changes in a single muscular contraction can be well observed by this method.

Über die Hemmung von Desoxyribonucleotide spaltenden Fermenten durch Colchicin

1949

The authors raise the question, if enzymatic processes possibly linked with the mitotic cell division may be influenced by mitotic poisons. The presented date show an inhibition of the dephosphorylation of desoxyribonucleotides at a rate of about 50% and of the deamination of about 40% by colchicine (final concentration 1·2·10−2M).

Differential incorporation of labeled amino acids in the territories of the sea urchin blastula

1962

Blastule con mesenchima primario diParacentrotus lividus esposte a un trattamento di 30 min con S35-metionina, leucina-C14 e alanina-C14 mostrano nel territorio vegetativo una densita di tracce considerevolmente minore che in quello animale. Se gli embrioni sono invece esposti all'aminoacido radioattivo durante tutto lo sviluppo o durante le prime ore dopo la fecondazione, tale differenza tra territori animale e vegetativo non si osserva. Si discute il significato di questi reperti.

Effects of Kaempferol and Myricetin on Inducible Nitric Oxide Synthase Expression and Nitric Oxide Production in Rats

2010

Abstract:  When administered as drugs or consumed as food components, polyphenolic compounds synthesized in plants interfere with intracellular signal transduction pathways, including pathways of nitric oxide synthase expression. However, effects of these compounds in vivo do not always correlate with nitric oxide synthase-inhibiting activities revealed in experiments with cultured cells. The initial goal of this work was to compare effects of flavonoids kaempferol and myricetin on inducible nitric oxide synthase mRNA and protein expression monitored by real-time RT-PCR and immunohistochemistry and to evaluate the impact of these effects on nitric oxide production in rat organs measured by …

Purification of a nuclease from human serum.

1981

The purification procedure for a nuclease from human serum is described. It includes ammonium sulfate precipitation, chromatography on DEAE-Sephadex and on Sephacryl-S 200, and preparative electrophoresis. The enzyme, purified about 2000-fold, is homogeneous in a sodium dodecyl sulfate electrophoretic system, where it has a mol. wt of 78,000. The pH optimum lies around pH 6.5; it is a sugar-nonspecific endonuclease.

Untersuchungen über den Einfluss verschiedener Kationen auf die ATP-Spaltung durch die Zytoplasmafraktion vom Plexus chorioideus des Rindes

1967

The cytoplasma fraction of the bovine choroid plexus epithelial cells was found to contain a considerable ATPase activity. The influence of Na+, K+, Li+, Rb+, Cs+, Co++, Mn++, Zn++ and Fe+++ on the activity of the Mg++-dependent enzyme has been studied. The monovalent cations do not influence the enzymic activity, whereas the effect of the bi- and trivalent cations is characterized by an inhibition of the ATPase.

On a glycoprotein of the sea urchin eggs and its changes following fertilization.

1960

Sbattendo con etere la frazione solubile in acido tricloroacetico di uova vergini diParacentrotus lividus si forma un precipitato all'interfacie tra etere e fase acquosa. L'analisi di questo precipitato ha dimostrato che si tratta di una glicoproteina. Il precipitato non si forma da estratti di uova fecondate.