Purification of a nuclease from human serum.

6533b829fe1ef96bd128a2f8

RESEARCH PRODUCT

Purification of a nuclease from human serum.

Hanoch Slor Hanoch Slor R. K. Zahn R. K. Zahn G. Seibert G. Seibert E. J. Zöllner E. J. Zöllner

subject

Pharmacology chemistry.chemical_classification Nuclease Chromatography Deoxyribonucleases biology Cell Biology Cellular and Molecular Neuroscience Electrophoresis chemistry.chemical_compound Endonuclease Kinetics Enzyme chemistry biology.protein Molecular Medicine Humans Ammonium Sodium dodecyl sulfate Sulfate Molecular Biology Ammonium sulfate precipitation

description

The purification procedure for a nuclease from human serum is described. It includes ammonium sulfate precipitation, chromatography on DEAE-Sephadex and on Sephacryl-S 200, and preparative electrophoresis. The enzyme, purified about 2000-fold, is homogeneous in a sodium dodecyl sulfate electrophoretic system, where it has a mol. wt of 78,000. The pH optimum lies around pH 6.5; it is a sugar-nonspecific endonuclease.

year	journal	country	edition	language
1981-06-01	Experientia

10.1007/bf01990041 https://pubmed.ncbi.nlm.nih.gov/7262274

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