0000000000542168

AUTHOR

R. K. Zahn

showing 6 related works from this author

Gas-chromatographische Bestimmung des DNS-Gehaltes biologischen Materials über den Thymingehalt

1970

ChemistryEnvironmental chemistryClinical BiochemistryGeneral Materials ScienceGeneral MedicineAnalytical ChemistryFresenius' Zeitschrift für analytische Chemie
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Purification of a nuclease from human serum.

1981

The purification procedure for a nuclease from human serum is described. It includes ammonium sulfate precipitation, chromatography on DEAE-Sephadex and on Sephacryl-S 200, and preparative electrophoresis. The enzyme, purified about 2000-fold, is homogeneous in a sodium dodecyl sulfate electrophoretic system, where it has a mol. wt of 78,000. The pH optimum lies around pH 6.5; it is a sugar-nonspecific endonuclease.

Pharmacologychemistry.chemical_classificationNucleaseChromatographyDeoxyribonucleasesbiologyCell BiologyCellular and Molecular NeuroscienceElectrophoresischemistry.chemical_compoundEndonucleaseKineticsEnzymechemistrybiology.proteinMolecular MedicineHumansAmmoniumSodium dodecyl sulfateSulfateMolecular BiologyAmmonium sulfate precipitationExperientia
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Genotoxicity of the fungicide dichlofluanid in seven assays

1991

Seven different endpoints for detection of genotoxicity have been used to demonstrate the DNA-altering properties of Dichlofluanid, a fungicide commonly used in viticulture pest control. Each endpoint (DNA synthesis inhibition test, alkaline viscosimetry, umu-test, alkaline filter elution, FADU-test, 32P-postlabeling, and electron microscopy) shows clear evidence of genotoxicity. These data indicate that application of the fungicide dichlofluanid may be mutagenic and/or carcinogenic for exposed humans.

DNA ReplicationSalmonella typhimuriumDNA AlterationEpidemiologyHealth Toxicology and MutagenesisDichlofluanidmedicine.disease_causeCell LineMicechemistry.chemical_compoundmedicineAnimalsHumansBioassayGenetics (clinical)CaptanCarcinogenAniline CompoundsMutagenicity TestsFishesDNAPesticideFungicides IndustrialFungicideBiochemistrychemistryGenotoxicityDNA DamageHeLa CellsMutagensEnvironmental and Molecular Mutagenesis
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Desoxyribonuclease-Aktivität im Seminalplasma von normalem und pathologischem Sperma1

2009

Zusammenfassung Das Verteilungsmuster der DNase-Aktivitaten im Seminalplasma von 9 Normalpersonen und 18 Patienten mit einem pathologischen Spermabefund wurde mit dem mikro-disc-elektrophoretischen Verfahren untersucht. Dabei ergaben die Aktivitatsbestimmungen der DNase bei Patienten mit einer Oligoasthenoteratozoospermie als auch bei alien Probanden, die einen pathologischen Spermabefund aufwiesen, in der 1. und 2. Bande deutliche Aktivitatsminderungen im Vergleich zum Seminalplasma von Normalpersonen. Nach den vorliegenden Untersuchungsbefunden stellt sich die Frage, ob den DNasen im Seminalplasma die Aufgabe zukommt, geschadigte und uberalterte Spermatozoen, deren Membran permeabler gewo…

Gynecologymedicine.medical_specialtyEndocrinologybusiness.industryUrologyDistribution patternmedicineHealthy subjectsGeneral MedicinebusinessAndrologia
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A Headful of T4 Coliphage DNA Packaged to Fasces-Like Globules in Fractal Models

1998

We present a new model for T4 DNA packaging based on fractal considerations. The proposed model is based on electron microscopic observations of spread and packaged DNA. The model takes into account enzymatically and unidirectionally driven packaging, and quick release of the DNA during infection. We also consider the different biochemical reactions of the packaging process.

chemistry.chemical_compoundFractalMaterials sciencechemistrybiologyBiophysicsActive packagingBiochemical reactionsColiphagebiology.organism_classificationElectron microscopicDna packagingDNA
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Determination of radioactivity-labelled derivatives of [8-14C]-6-mercaptopurine in homogenates of L5178Y mouse lymphoma cells using high-pressure liq…

1976

ChemistryClinical BiochemistryGeneral Materials ScienceGeneral MedicineBiochemistryAnalytical ChemistryFresenius' Zeitschrift f�r Analytische Chemie
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