Search results for "CHON"

Electron Microscopic Contrast of the Cytoskeleton and Junctional Complexes of Intestinal Epithelial Cells by Ethanolic Phosphotungstic Acid

2000

After glutaraldehyde fixation and treatment with ethanolic phosphotungstic acid (E-PTA) before plastic embedding, sections of rat large intestine showed a characteristic electron contrasting pattern in epithelial cells. The axis of microvilli, terminal web, a thin band below the luminal plasma membrane, centrioles and junctional complexes (tight junctions, adherens junctions, and desmosomes) appeared highly contrasted. In addition to protein components of microfilaments and intermediate filaments, proteins from the junctional complexes could also be implicated in the contrasting reaction with E-PTA. Mitochondrial membranes, chromatin masses, and nucleoli of enterocytes showed considerable e…

Dichrorampha dinarica, new species, a century of confusion in European lepidopterology (Lepidoptera: Tortricidae) resolved by combining morphology an…

2012

Dichrorampha dinarica, new species, is described from the Dinaric mountains (Macedonia, Bosnia and Herzegovina). The newspecies was formerly confused with D. ligulana (Herrich-Schäffer, 1851) from the Alps and D. rilana Drenowsky, 1909 fromthe Rila mountains (Bulgaria). Therefore, a re-description of D. rilana is provided based on topotypical specimens. The diag-nostic morphological characters of the involved species, supported by significant divergence of the mtDNA barcode (COI fragment of 658 bp), are discussed in detail.

Peroxisome proliferators and peroxisome proliferator activated receptors (PPARs) as regulators of lipid metabolism.

1997

Peroxisome proliferation (PP) in mammalian cells, first described 30 years ago, represents a fascinating field of modern research. Major improvements made in its understanding were obtained through basic advances that have opened up new areas in cell biology, biochemistry and genetics. A decade after the first report on PP, a new metabolic pathway (peroxisomal beta-oxidation) and its inducibility by peroxisome proliferators were discovered. More recently, a new type of nuclear receptor, the peroxisome proliferator-activated receptor (PPAR), has been described. The first PPAR was discovered in 1990. Since then, many other PPARs have been characterized. This original class of nuclear receptor…

Heme oxygenase-1 induction modulates microsomal prostaglandin E synthase-1 expression and prostaglandin E2 production in osteoarthritic chondrocytes

2009

Pro-inflammatory cytokines such as interleukin-1beta (IL-1beta) may participate in the pathogenesis of cartilage damage in osteoarthritis (OA) through the production of catabolic enzymes and inflammatory mediators. Induction of heme oxygenase-1 (HO-1) has previously been shown to exert anti-inflammatory effects in different cell types. We have investigated whether HO-1 induction may modify chondrocyte viability and the production of relevant mediators such as oxidative stress and prostaglandin E(2) (PGE(2)) elicited by IL-1beta in OA chondrocytes. Chondrocytes were isolated from OA cartilage and used in primary culture. Cells were stimulated with IL-1beta in the absence or presence of the H…

Overexpression of Human and Fly Frataxins in Drosophila Provokes Deleterious Effects at Biochemical, Physiological and Developmental Levels

2011

10 pages, 5 figures. 21779322[PubMed] PMCID: PMC3136927

FP187MITOCHONDRIAL DYSFUNCTION INDUCED BY TENOFOVIR IN RENAL CELLS. POTENTIATION OF THE EFFECTS BY CO-STIMULATION WITH ANGIOTENSIN II

2015

Quantitative real-time ARMS-qPCR for mitochondrial DNA enables accurate detection of microchimerism in renal transplant recipients

2011

Hoerning A, Kalkavan H, Rehme C, Menke J, Worm K, Garritsen HSP, Buscher R, Hoyer PF. Quantitative real-time ARMS-qPCR for mitochondrial DNA enables accurate detection of microchimerism in renal transplant recipients. Pediatr Transplantation 2011: 15: 809–818. © 2011 John Wiley & Sons A/S. Abstract:  The presence of microchimerism in peripheral blood of solid organ transplant recipients has been postulated to be beneficial for allograft acceptance. Kinetics of donor cell trafficking and accumulation in pediatric allograft recipients are largely unknown. In this study, we implemented SNPs of the HVRs I and II of mitochondrial DNA to serve as molecular genetic markers to detect donor-specific…

Origin of SiO2-rich components in ordinary chondrites

2006

Abstract Silica-rich objects are common minor components in ordinary chondrites (OC), occurring as fragments and as chondrules. Their typical paragenesis is orthopyroxene + SiO 2 (with bulk SiO 2 >65 wt%) and occasionally with additional olivine and/or spinel. Individual silica-rich components (SRC) have previously been studied in various types of OCs, although there is only one comprehensive study of these objects by Brigham et al. [Brigham, C.A., Murrell, M.T., Yabuki, H., Ouyang, Z., El Goresy, A., 1986. Silica-bearing chondrules and clasts in ordinary chondrites. Geochim. Cosmochim. Acta 50, 1655–1666]. Several different explanations of how SRCs formed have been published. The main ques…

Design and experimental test of a low costweather buoy

2013

The importance of prevention plans based on realistic inundation scenarios was dramatically highlighted by the Indian Ocean Tsunami of 26 December 2004. But also in the Mediterranean, sea storms basin are a quite common event especially during winter months. These scenarios are generally based on numerical models of tsunami wave propagation that can become more realistic and effective when calibrated with the distribution of true data acquired by special weather buoy. In this paper we present the result of an experimental weather buoy built by DEIM of University of Palermo, that is characterized by small cost and big versatility.

Possible Mechanisms for Tumour Cell Sensitivity to TNF-a and Potential Therapeutic Applications

2001

TNF is a macrophage / monocyte-derived cytokine with cytostatic and cytotoxic anti-tumour activity. TNF-alpha can cause haemorrhagic necrosis and regression of experimental tumours. Nevertheless, the TNF-alpha doses required to cure tumour-bearing mice lead to injury of normal tissues and, eventually, may cause a lethal shock syndrome. This toxicity implies severe limitations for the therapeutic use of TNF-a. Reactive oxygen intermediates (ROIs) are involved in TNF-a-induced cell killing. Different studies are consistent with the hypothesis that tumour cell sensitivity to TNF-alpha is related to its capacity to buffer oxidative attack. Recently, we have demonstrated that the sensitivity of …