Search results for "Caprolactone"
showing 10 items of 72 documents
Covalent RGD modification of the inner pore surface of polycaprolactone scaffolds
2011
Scaffold production for tissue engineering was demonstrated by means of a hot compression molding technique and subsequent particulate leaching. The utilization of spherical salt particles as the pore-forming agent ensured complete interconnectivity of the porous structure. This method obviated the use of potentially toxic organic solvents. To overcome the inherent non-cell-adhesive properties of the hydrophobic polymer polycaprolactone (PCL) surface activation with a diamine was performed, followed by the covalent immobilization of the adhesion-promoting RGD-peptide. The wet-chemical approach was performed to guarantee modification throughout the entire scaffold structure. The treatment wa…
Integration of PCL and PLA in a monolithic porous scaffold for interface tissue engineering.
2016
A novel bi-layered multiphasic scaffold (BLS) have been fabricated for the first time by combining melt mixing, compression molding and particulate leaching. One layer has been composed by polylactic acid (PLA) presenting pore size in the range of 90-110µm while the other layer has been made of polycaprolactone (PCL) with pores ranging from 5 to 40µm. The different chemo-physical properties of the two biopolymers combined with the tunable pore architecture permitted to realize monolithic functionally graded scaffolds engineered to be potentially used for interface tissues regenerations. BLS have been characterized from a morphological and a mechanical point of view. In particular, mechanica…
Electrospun Polyhydroxyethyl-Aspartamide-Polylactic Acid Scaffold for Biliary Duct Repair: A Preliminary In Vivo Evaluation
2017
Abstract Tissue engineering has emerged as a new approach with the potential to overcome the limitations of traditional therapies. The objective of this study was to test whether our polymeric scaffold is able to resist the corrosive action of bile and to support a cell's infiltration and neoangiogenesis with the aim of using it as a biodegradable tissue substitute for serious bile duct injuries. In particular, a resorbable electrospun polyhydroxyethyl-aspartamide–polylactic acid (90 mol% PHEA, 10 mol% PLA)/polycaprolactone (50:50 w/w) plate scaffold was implanted into rabbit gallbladder to assess the in vivo effects of the lytic action of the bile on the scaffold structure and then as a tu…
Heparin functionalized polyaspartamide/polyester scaffold for potential blood vessel regeneration
2013
An interesting issue in tissue engineering is the development of a biodegradable vascular graft able to substitute a blood vessel and to allow its complete regeneration. Here, we report a new scaffold potentially useful as a synthetic vascular graft, produced through the electrospinning of α,β-poly(N-2-hydroxyethyl) (2-aminoethylcarbamate)-D,L-aspartamide-graft-polylactic acid (PHEA-EDA-g-PLA) in the presence of polycaprolactone (PCL). The scaffold degradation profile has been evaluated as well as the possibility to bind heparin to electrospun fibers, being it a known anticoagulant molecule able to bind growth factors. In vitro cell compatibility has been investigated using human vascular e…
Ring opening polymerization of ε-caprolactone initiated by titanium and vanadium complexes of ONO-type schiff base ligand
2021
AbstractA phenoxy-imine proligand with the additional OH donor group, 4,6-tBu2-2-(2-CH2(OH)-C6H4N = CH)C6H3OH (LH2), was synthesized and used to prepare group 4 and 5 complexes by reacting with Ti(OiPr)4 (LTi) and VO(OiPr)3 (LV). All new compounds were characterized by the FTIR, 1H and 13C NMR spectroscopy and LTi by the single-crystal X-ray diffraction analysis. The complexes were used as catalysts in the ring opening polymerization of ε-caprolactone. The influence of monomer/transition metal molar ratio, reaction time, polymerization temperature as well as complex type was investigated in detail. The complexes showed high (LTi) and moderate (LV) activity in ε-caprolactone polymerization a…
Conductive polycaprolactone/gelatin/polyaniline nanofibres as functional scaffolds for cardiac tissue regeneration
2022
Abstract The endorsement of functional features such as biocompatibility, mechanical integrity, or electrical conductivity to tissue engineering (TE) scaffolds is essential to stimulate cell adhesion and proliferation. In this study, electrospun nanofibers based on polycaprolactone (PCL) and gelatin (Ge) (ratios 60/40, 50/50, and 40/60), and polyaniline (PAni) particles (0.25, 0.50, and 1.00%wt) were prepared. The time of dissolution in an acid solvent mixture before electrospinning allowed for obtaining nanofibers with controlled features. Changes in the molar mass (Mn from 90·103 to 15·103 g·mol−1), in the crystalline microstructure (Xc from 60 to 25%) and the surface morphology (diameter…
GALACTOSE-DECORATED POLYMERIC CARRIERS FOR HEPATOCYTE-SELECTIVE DRUG TARGETING
2015
In this paper, the current available strategies to realize galactose-decorated nanostructured polymeric systems are summarized. These carriers are designed in order to obtain targeted drug delivery to hepatocytes via galactose (GAL) moieties, i.e., for the treatment of viral hepatitis or liver cancer that are the greater causes of global disability and mortality. Usually, the main followed strategy to obtain galactosylated polymeric carriers is to use galactosylated copolymers. The chemical modifications of preformed polymers with sugar-containing reagents is followed for obtaining lactosaminated human albumin, galactosylated phospholipid-polyaminoacid and polylactide (PLA)- polyaminoacid c…
Improvement of actinorhodin production yield in Streptomyces coelicolor by immobilized-cell cultivations by using PCL- and PLA-based films
2016
Actinomycetes are Gram-positive bacteria producing most of naturally occurring antibiotics (Donadio et al., 2010). At industrial level, antibiotics are produced by submerged fermentations where the actinomycete filamentous morphology negatively affects bioproductivity (van Dissel et al., 2014). Microporous membranes for bacterial cell-immobilization were already proven increasing bioproductivity in Streptomyces coelicolor, that is a model actinomycete producing the blue pigmented actinorhodin (ACT) antibiotic (Scaffaro et al., 2016). To develop an immobilized-cell bioreactor system, different kinds of polycaprolactone (PCL) and polylactic acid (PLA) films were produced by an electrospinning…
Actinorhodin production intensification by nanofibrous membranes in Streptomyces coelicolor cultures
2016
In this work, electrospun polycaprolactone (PCL) and polylactic acid (PLA) membranes, subjected or not to O2-plasma treatment, werwe used as support for cell-immobilization in S. coelicolor immobilized-cells created a compact biofilm on both kinds of membranes.