Search results for "Cattle"

showing 10 items of 608 documents

REDUCTION OF NILUTAMIDE BY NO SYNTHASES : IMPLICATIONS FOR THE ADVERSE EFFECTS OF THIS NITROAROMATIC ANTIANDROGEN DRUG

2003

Nitric oxide synthases (NOSs) are flavohemeproteins that catalyze the oxidation of l-arginine to l-citrulline with formation of the widespread signal molecule NO. Beside their fundamental role in NO biosynthesis, these enzymes are also involved in the formation of reactive oxygen species and in the interactions with some xenobiotic compounds. Nilutamide is a nonsteroidal antiandrogen that behaves as a competitive antagonist of the androgen receptors and is proposed in the treatment of metastatic prostatic carcinoma. However, therapeutic effects of nilutamide are overshadowed by the occurrence of several adverse reactions mediated by toxic mechanism(s), which remain(s) poorly investigated. H…

Time FactorsFree RadicalsNitric Oxide Synthase Type IIImedicine.drug_class[CHIM.THER] Chemical Sciences/Medicinal ChemistryNitric Oxide Synthase Type IINitric Oxide Synthase Type I[CHIM.THER]Chemical Sciences/Medicinal ChemistryToxicologyAntiandrogenImidazolidinesNitric oxide03 medical and health scienceschemistry.chemical_compoundMice0302 clinical medicineHydroxylaminemedicineAnimalsAnaerobiosisAmines030304 developmental biologychemistry.chemical_classification0303 health sciencesReactive oxygen speciesElectron Spin Resonance SpectroscopyImidazolesAndrogen AntagonistsGeneral MedicineRecombinant Proteins3. Good healthRatsAndrogen receptorEnzymechemistryBiochemistryCompetitive antagonist030220 oncology & carcinogenesisNilutamideCattleNitric Oxide SynthaseOxidation-ReductionNADPmedicine.drug
researchProduct

Biodegradation of differently cross-linked collagen membranes: an experimental study in the rat.

2005

Contains fulltext : 47774.pdf (Publisher’s version ) (Closed access) The aim of the present study was to compare the biodegradation of differently cross-linked collagen membranes in rats. Five commercially available and three experimental membranes (VN) were included: (1) BioGide (BG) (non-cross-linked porcine type I and III collagens), (2) BioMend (BM), (3) BioMendExtend (BME) (glutaraldehyde cross-linked bovine type I collagen), (4) Ossix (OS) (enzymatic-cross-linked bovine type I collagen), (5) TutoDent (TD) (non-cross-linked bovine type I collagen, and (6-8) VN(1-3) (chemical cross-linked porcine type I and III collagens). Specimens were randomly allocated in unconnected subcutaneous po…

Tissue engineering and reconstructive surgery [UMCN 4.3]Time FactorsSwineForeign-Body ReactionBovine Type I CollagenTissue integrationCollagen membraneMembranes ArtificialAnatomyBiodegradationRatsAndrologychemistry.chemical_compoundMembranechemistryAbsorbable ImplantsAnimalsAnimal studyCattleGlutaraldehydeCollagenOral SurgeryRats Wistar
researchProduct

Functional coupling of nitric oxide synthase and soluble guanylyl cyclase in controlling catecholamine secretion from bovine chromaffin cells

1997

This study was designed to evaluate whether the enzymes of the nitric oxide/cyclic-GMP pathway, nitric oxide synthase and soluble guanylyl cyclase, are functionally coupled in controlling catecholamine secretion in primary cultures of bovine chromaffin cells. In immunocytochemical studies, 80-85% of the tyrosine hydroxylase-positive chromaffin cells also possessed phenylethanolamine-N-methyltransferase, f1p4cating their capability to synthesize epinephrine. Immunoreactivity for neuronal-type nitric oxide synthase was found in over 90% of all chromaffin cells. Reverse transcription-polymerase chain reaction also demonstrated neuronal-type nitric oxide synthase messenger RNA. Immunoreactivity…

Tyrosine 3-MonooxygenaseChromaffin CellsPolymerase Chain ReactionNitric oxidechemistry.chemical_compoundCatecholaminesCytosolAdrenal GlandsmedicineAnimalsRNA MessengerCyclic GMPbiologyChemistryPhenylethanolamine N-MethyltransferaseGeneral NeuroscienceNitric oxide synthasemedicine.anatomical_structureBiochemistryGuanylate CyclaseChromaffin cellCatecholaminebiology.proteinCalciumCattleSodium nitroprussideNitric Oxide SynthaseAdrenal medullaSoluble guanylyl cyclaseAcetylcholinemedicine.drugNeuroscience
researchProduct

Analysis of β2-agonists in cattle hair samples using a rapid UHPLC–ESI–MS/MS method

2016

A simple and efficient method was developed for simultaneous analysis of five illegal residual β2-agonists in cattle hair. β2-Agonists were quantified by ultra high performance liquid chromatography coupled with electrospray ionisation tandem mass spectrometry operating in positive multiple-reaction monitoring mode. The method was validated as quantitative confirmatory method according to the EU Decision 2002/657/EC: instrumental linearity, specificity, precision, recovery, decision limit (CCα) and detection capability (CCβ) were evaluated. The recovery were greater than 90% and the method appeared suitable for the control of these β2-agonists in cattle hair samples with LOQ values between …

UHPLC –ESI–MS/MSβ2-agonist0301 basic medicineElectrospray030109 nutrition & dieteticsChromatographycattle hair; Commission Decision 2002/657/EC; UHPLC–ESI–MS/MS; β2-agonists; Plant Science; Biochemistry; Analytical Chemistry; Organic ChemistryChemistryβ2 agonistsElectrospray ionization010401 analytical chemistryOrganic ChemistryPlant ScienceContaminationTandem mass spectrometry01 natural sciencesBiochemistry0104 chemical sciencesAnalytical Chemistry03 medical and health sciencesCommission Decision 2002/657/ECcattle hairAdrenergic beta-AgonistsUltra high performanceDecision limitNatural Product Research
researchProduct

Novel inhibitors of mitochondrial respiratory chain: endoperoxides from the marine tunicate Stolonica socialis.

2001

The Mediterranean tunicate Stolonica socialis contains a new class of powerful cytotoxic acetogenins, generically named stolonoxides. In this paper, which also details the isolation and chemical characterization of a minor component (3a) of the tunicate extract, we report the potent inhibitory activity (IC(50) < 1 microM) of stolonoxides (1a and 3a) on mitochondrial electron transfer. The compounds affect specifically the functionality of complex II (succinate:ubiquinone oxidoreductase) and complex III (ubiquinol:cytochrome C oxidoreductase) in mammalian cells, thereby causing a rapid collapse of the whole energetic metabolism. This result, which differs from the properties of similar known…

UbiquinolMagnetic Resonance SpectroscopyStereochemistryIn Vitro TechniquesFunctional activityElectron Transportchemistry.chemical_compoundElectron Transport Complex IIIMarine Natural ProductOxidoreductaseMultienzyme ComplexesDrug DiscoveryMediterranean SeaAnimalsNADH NADPH OxidoreductasesUrochordataEnzyme InhibitorsFuranschemistry.chemical_classificationElectron Transport Complex IbiologyCytochrome cElectron Transport Complex IISuccinate dehydrogenaseElectron Transport Complex IIMyocardiumDioxolanesMitochondriaPeroxidesSuccinate DehydrogenaseMitochondrial respiratory chainchemistryBiochemistryElectron Transport Complex ICoenzyme Q – cytochrome c reductasebiology.proteinMolecular MedicineCattleStructure ElucidationOxidoreductasesJournal of medicinal chemistry
researchProduct

Kinetic characterization of mitochondrial complex I inhibitors using annonaceous acetogenins

1999

The NADH:ubiquinone oxidoreductase (complex I) of the mitochondrial respiratory chain is by far the largest and most complicated of the proton-translocating enzymes involved in the oxidative phosphorylation. Many clues regarding the electron pathways from matrix NADH to membrane ubiquinone and the links of this process with the translocation of protons are highly controversial. Different types of inhibitors become valuable tools to dissect the electron and proton pathways of this complex enzyme. Therefore, further knowledge of the mode of action of complex I inhibitors is needed to understand the underlying mechanism of energy conservation. This study presents for the first time a detailed …

UbiquinoneSubmitochondrial ParticlesBiophysicsOxidative phosphorylationBiologyBiochemistryMitochondria HeartLactonesOxidoreductaseRotenoneNAD(P)H Dehydrogenase (Quinone)Mammalian enzymeAnimalsFuransMolecular Biologychemistry.chemical_classificationNADH-Ubiquinone OxidoreductasePlant ExtractsNADKineticsMitochondrial respiratory chainEnzymechemistryBiochemistryCattleAnnonaceous AcetogeninsMitochondrial Complex I
researchProduct

Synthetic vaccines consisting of tumor-associated MUC1 glycopeptide antigens and bovine serum albumin.

2005

Vaccines SyntheticbiologyChemistryMolecular Sequence DataMucin-1Serum albuminGlycopeptidesSerum Albumin BovineStereoisomerismGeneral ChemistryCancer VaccinesCatalysisGlycopeptideBiochemistryAntigenCarbohydrate Sequencebiology.proteinCarbohydrate ConformationAnimalsHumansCattleCarbohydrate conformationBovine serum albuminMUC1Angewandte Chemie (International ed. in English)
researchProduct

Synthetic vaccines of tumor-associated glycopeptide antigens by immune-compatible thioether linkage to bovine serum albumin.

2007

Vaccines SyntheticbiologyMolecular StructureGlycopeptidesSerum Albumin BovineGeneral ChemistrySulfidesCancer VaccinesCatalysisGlycopeptidechemistry.chemical_compoundImmune systemBiochemistryAntigenThioetherchemistryAntigens Neoplasmbiology.proteinAnimalsCattleBovine serum albuminAngewandte Chemie (International ed. in English)
researchProduct

Comparison of growth &amp; function of endothelial progenitor cells cultured on deproteinized bovine bone modified with covalently bound fibronectin …

2016

Objectives The objective of this study was to assess and compare the growth and function of Endothelial Progenitor Cells (EPCs) cultured on covalently bonded Vascular Endothelial Growth Factor (VEGF) and covalently bonded Fibronectin (FN) coating on deproteinized bovine bone (DBB) (test samples), compared to non-modified DBB blocks (control sample). Materials and methods The test samples were prepared by plasma polymerization of allylamine onto DBB blocks. Group1 of test samples were prepared with VEGF coating (VEGF-DBB) where as the Group2 test samples were coated with FN (FN-DBB). Non-modified DBB blocks served as a Control. EPCs were isolated and cultivated from buffy coats of peripheral…

Vascular Endothelial Growth Factor ANitric Oxide Synthase Type IIIAngiogenesis0206 medical engineeringNitric Oxide Synthase Type IICell CountEnzyme-Linked Immunosorbent Assay02 engineering and technologyReal-Time Polymerase Chain ReactionCell morphologyAllylamine03 medical and health scienceschemistry.chemical_compound0302 clinical medicineEnosAnimalsHumansMTT assayProgenitor cellCells CulturedCell ProliferationEndothelial Progenitor CellsMicroscopy Confocalbiology030206 dentistrybiology.organism_classification020601 biomedical engineeringMolecular biologyFibronectinsVascular endothelial growth factorFibronectinchemistryBone SubstitutesImmunologybiology.proteinCattleOral SurgeryClinical Oral Implants Research
researchProduct

Characterization of Membrane-Bound Cyclic Nucleotide Phosphodiesterases from Bovine Aortic Smooth Muscle

1992

This study reports the isolation and characterization of cyclic nucleotide phosphodiesterases (PDEs) associated with membrane fraction in comparison to cytosolic forms from bovine aorta. DEAE-Sephacel chromatography of a solubilized membrane fraction from a homogenate, prepared under isotonic conditions in the presence of protease inhibitors, yielded one major peak of PDE activity that specifically hydrolyzed cAMP and was not stimulated by calmodulin: It appeared to contain two subtypes of PDE. The first subtype belonged to the cyclic GMP (cGMP)-inhibited PDE family, (PDE III): It had an apparent Km value of 0.4 microM and was potently inhibited by cGMP, LY186126, and cilostamide. The secon…

Vascular smooth muscleCalmodulinPhosphodiesterase InhibitorsMuscle Smooth Vascularchemistry.chemical_compoundCytosolCalmodulinCyclic AMPmedicineAnimalsheterocyclic compoundsCyclic GMPRolipramPharmacologyCilostamidebiologyCyclic nucleotide phosphodiesterasePhosphoric Diester HydrolasesHydrolysisCell MembraneBiological membranemusculoskeletal systemenzymes and coenzymes (carbohydrates)Mechanism of actionBiochemistrychemistryEnzyme inhibitorbiology.proteinCattleChromatography Thin Layersense organsmedicine.symptomCardiology and Cardiovascular Medicinecirculatory and respiratory physiologymedicine.drugJournal of Cardiovascular Pharmacology
researchProduct