Search results for "Chip"
showing 10 items of 386 documents
Revisited BIA-MS combination: Entire "on-a-chip" processing leading to the proteins identification at low femtomole to sub-femtomole levels
2008
International audience; We present the results of a study in which biomolecular interaction analysis (BIA, Biacore 2000) was combined with mass spectrometry (MS) using entire "on-a-chip" procedure. Most BIA-MS studies included an elution step of the analyte prior MS analysis. Here, we report a low-cost approach combining Biacore analysis with homemade chips and MS in situ identification onto the chips without elution step. First experiments have been made with rat serum albumin to determine the sensitivity and validation of the concept has been obtained with an antibody/antigen couple. Our "on-a-chip" procedure allowed complete analysis by MS-MS of the biochip leading to protein identificat…
Microarray analysis in sperm from fertile and infertile men without basic sperm analysis abnormalities reveals a significantly different transcriptom…
2007
Sperm analysis following World Health Organization guidelines is unable to explain the molecular causes of male infertility when basic sperm parameters are within a normal range and women do not present gynecologic pathology. Consequently, there is a need for accurate diagnostic tools in this area, and microarray technology emerges as promising. We present, for the first time, preliminary results of a comparison of sperm mRNA expression profiles between fertile and infertile men with normal semen parameters, discovering profound discrepancies between groups, with potential diagnostic and therapeutic possibilities.
Luminometric Sub-nanoliter Droplet-to-droplet Arrays (LUMDA-chip) for Drug Screening on Phase I Metabolism Enzymes
2013
Here we show the fabrication of the Luminometric Sub-nanoliter Droplet-to-droplet Array (LUMDA chip) by inkjet printing. The chip is easy to be implemented and allows for amultiplexed multi-step biochemical assay in sub-nanoliter liquid spots. This concept is here applied to the integral membrane enzyme CYP3A4, i.e. themost relevant enzymatic target for phase I drug metabolism, and to some structurally-related inhibitors.
Model and Control Strategy Simulation of a Racing Series Hybrid Car
2014
Action-reaction principle of Energetic Macroscopic Representation (EMR) and its Inversion Based Control (IBC) is used to organize subsystems interconnection of Noao, a plug-in series hybrid race car equipped with lithium-ion battery pack and an engine/generator set as its range extender, according to the physical causality. Results from drive test of the real car are used to validate the model. The objective of this paper is to provide a dynamic model of this car in order to improve its control scheme and analyse the impact of its optimisation.
Advanced Monitoring Systems Based on Battery-Less Asset Tracking Modules Energized through RF Wireless Power Transfer
2020
Asset tracking involving accurate location and transportation data is highly suited to wireless sensor networks (WSNs) featuring battery-less nodes that can be deployed in virtually any environment and require little or no maintenance. In response to the growing demand for advanced battery-less sensor tag solutions, this article presents a system for identifying and monitoring the speeds of assets in a WSN with battery-less tags that receive all their operating energy through radio frequency (RF) wireless power transfer (WPT) architecture, and a unique measurement approach to generate time-domain speed readouts. The assessment includes performance characteristics and key features of a syste…
Additions to knowledge of the biodiversity of benthic hydroids (Cnidaria: Hydrozoa) in the Balleny Islands (Antarctica)
2021
The Balleny Islands, an isolated archipelago located 240 km off the coast of Victoria Land (East Antarctica), are separated from the Antarctic continental shelf by waters more than 2000 m deep. The benthic hydroid fauna of these islands is little known, with only 25 species reported so far. The present study contributes to a better knowledge of the benthic hydrozoans inhabiting this archipelago by studying a small collection held at the National Institute of Water and Atmospheric Research Invertebrate Collection at Wellington, New Zealand. Sixteen species of benthic hydroids were found in the collection, belonging to nine families and 12 genera of Leptothecata. Kirchenpaueriidae and Oswalde…
Contributo alla conoscenza del genere Tamarix L. nell'Isola del Giglio (Arcipelago Toscano)
2009
Gli Autori, sulla base di una revisione dei campioni d'erbario depositati presso l'Università di Firenza e di personali raccolte, forniscono un contributo alla conoscenza delle tamerici dell'Isola del Giglio e propongono una chiave di identificazione dei taxa censiti.
I funghi dell'Isola del Giglio (Arcipelago Toscano): primo contributo
2010
The ecological and distributive data on macromycetes of Isola del Giglio (Tuscany Archipelago, ltaly), published in 1900 by Stefano Sommier; are integrated by new data on forty-live macromycetes. The recorded taxa are new for the island with the exception of seven species previously reported by Sommier.
A Tropical Archipelago
2017
The tropical maritime climate of the Philippines is marked by high temperature and abundant rainfall. The chapter first examines the major characteristics of the climate, dominated by the seasonal alternation of the Amihan and Habagat monsoons, leading to sharp differences between regions for their patterns of rainy seasons. Typhoons from the western Pacific hit the islands with brutal force, even if some years are more prone than others to the onslaught of tropical storms, depending on the general atmospheric circulation and the strength—or absence—of El Nino or La Nina. Typhoons not directly hitting the Philippines can still generate high levels of rainfall and enhance flooding, due to th…
Preparation of Biomolecule Microstructures and Microarrays by Thiol-ene Photoimmobilization
2009
A mild, fast and flexible method for photoimmobilization of biomolecules based on the light-initiated thiol-ene reaction has been developed. After investigation and optimization of various surface materials, surface chemistries and reaction parameters, microstructures and microarrays of biotin, oligonucleotides, peptides, and MUC1 tandem repeat glycopeptides were prepared with this photoimmobilization method. Furthermore, MUC1 tandem repeat glycopeptide microarrays were successfully used to probe antibodies in mouse serum obtained from vaccinated mice. Dimensions of biomolecule microstructures were shown to be freely controllable through photolithographic techniques, and features down to 5 …