Search results for "Cholera"
showing 7 items of 67 documents
Effects of adjuvants of the cholera toxin family on CD4 + T cell responses in a murine model of intrarectal immunization with rotavirus-like particles
2011
Mucosal immunization is an important goal of vaccine development to protect against pathogens that use mucosa as portals of entry. However, the use of non-replicating antigens requires the addition of adjuvants.Cholera-like enterotoxins, cholera toxin (CT) from Vibrio cholerae and the heat-labile enterotoxin (LT) from toxinogenic strains of E. coli, as well as the mutant LR-192G and their B subunits (CTB and LTB) have been shown to increase immune responses against unrelated co-administered antigens by mucosal routes. However, their mechanism of action is very complex and not completely understood and differences exist between holotoxins and B subunits and within molecules, differences exis…
The selective advantage of cystic fibrosis heterozygotes tested by aDNA analysis: A preliminary investigation
2000
Recently a heterozygote advantage was suggested to explain the high incidence (1:25 carrier individuals in Europeans) of the cystic fibrosis gene. This selective advantage was speculated to be due to a high resistance to chloride-secreting diarrhea, including cholera. Up to now the major efforts to test directly this hypothesis have been limited to animal models.
Identification of the membrane penetrating domain of Vibrio cholerae cytolysin as a β-barrel structure
2005
Summary Vibrio cholerae cytolysin (VCC) is an oligomerizing pore-forming toxin that is related to cytolysins of many other Gram-negative organisms. VCC contains six cysteine residues, of which two were found to be present in free sulphydryl form. The positions of two intramolecular disulphide bonds were mapped, and one was shown to be essential for correct folding of protoxin. Mutations were created in which the two free cysteines were deleted, so that single cysteine substitution mutants could be generated for site-specific labelling. Employment of polarity-sensitive fluorophores identified amino acid side-chains that formed part of the pore-forming domain of VCC. The sequence commenced at…
ChemInform Abstract: Polycondensed Nitrogen Heterocycles. Part 17. Isoxazolo(4,3-d)pyrazolo-(3,4-f)(1,2,3)triazepine. A New Ring System.
1988
The title compounds were prepared by nitration of compounds 2, reduction of the dinitro derivatives 4 and diazotization of the diamino derivatives 6 followed by an intramolecular coupling reaction. Compound 4a showed good activity against Salmonella cholerasuis and Clostridium perfringens bacteria.
Science and law in the time of cholera: the mystery of the biscuits in Torroja (1865)
2018
By focussing on a case of collective poisoning in 1865, four features of forensic cultures are analysed: technologies for detection, authorized voices, regulations in action and the predominant social anxieties. Local newspapers and archival documents kept in the medical academies of Barcelona and Madrid are analysed. The analysis shows the tensions between various types of proof employed in legal medicine and the ambiguities between epidemic diseases and poisoning accidents. In the first section, I review the fears caused by the cholera epidemic in 1865, which was the first explanation of the accident. The changes of popular perception are also discussed by analysing contemporary newspaper…
Intrarectal immunization with rotavirus 2/6 virus-like particles induces an antirotavirus immune response localized in the intestinal mucosa and prot…
2006
ABSTRACTRotavirus (RV) is the main etiological agent of severe gastroenteritis in infants, and vaccination seems the most effective way to control the disease. Recombinant rotavirus-like particles composed of the viral protein 6 (VP6) and VP2 (2/6-VLPs) have been reported to induce protective immunity in mice when administered by the intranasal (i.n.) route. In this study, we show that administration of 2/6-VLPs by the intrarectal (i.r.) route together with either cholera toxin (CT) or a CpG-containing oligodeoxynucleotide as the adjuvant protects adult mice against RV infection. Moreover, when CT is used, RV shedding in animals immunized by the i.r. route is even reduced in comparison with…
Genetic relationship between clinical and environmental Vibrio cholerae isolates in Tanzania: A comparison using repetitive extragenic palindromic (R…
2015
The bacterium causing cholera, Vibrio cholerae, is a marine organism and coastal waters are important reservoirs of the organism. There are more than 200 serogroups of V. cholerae, of which serogroups O1 and O139 are known to be the causative agent of the cholera. The main virulent factor in V. cholerae is cholera toxin gene (ctx) that is found from the epidemic O1 and O139 strains, but may also be found in some strains other than O1 and O139 (non-O1 and non-O139). In this study, 48 V. cholerae strains isolated from three estuaries of Tanzania and 20 stool isolates were characterized in terms of their serogroups and possession of ctx gene and then compared using two PCR based fingerprinting…