Search results for "Clone"
showing 10 items of 312 documents
Synthesis and cell surface display of class II determinants by long-term propagated rat T line cells
1987
We have investigated the capacity of the encephalitogenic BS rat T cell line bs 83 and its variant clone bs 83.III.C6 to synthesize and express RT1.B-specific class II molecule subsets defined by monoclonal antibodies (mAb) MRC-OX6 and MRC-OX3. Earlier studies had indicated that mAb MRC-OX6 recognizes three distinct molecular species: an immature oligomeric polypeptide chain complex comprised of the polymorphic subunits alpha, beta and the invariant proteins of the gamma group; a biosynthetic intermediate composed of post-translationally modified alpha, beta and gamma chain (denoted p35) and a fully glycosylated alpha, beta two-chain complex derived from the plasma membrane. MRC-OX3 was sho…
Immunological Methods for Analysis of Recombinant Proteins
1998
We want to introduce researchers to techniques that help to solve some problems in the work of the molecular biologist. After transformation of recombinant DNA in E. coli cells many clones are usually produced, and the same situation appears if recombinant DNA expression libraries are available. Furthermore, if appropriate monoclonal or polyclonal antibodies are available, the method of immunoscreening of colonies for direct immunological detection of translational products of cloned genes can be used. Selected clones could be chosen for further studies such as determination of their primary structure by DNA sequencing and for characterization of an appropriate expressed protein.
In situ observations of CH<sub>2</sub>Cl<sub>2</sub> and CHCl<sub>3</sub…
2021
Abstract. Efficient transport pathways for ozone-depleting very short-lived substances (VSLSs) from their source regions into the stratosphere are a matter of current scientific debate; however they have yet to be fully identified on an observational basis. Understanding the increasing impact of chlorine-containing VSLSs (Cl-VSLSs) on stratospheric ozone depletion is important in order to validate and improve model simulations and future predictions. We report on a transport study using airborne in situ measurements of the Cl-VSLSs dichloromethane (CH2Cl2) and trichloromethane (chloroform, CHCl3) to derive a detailed description of two transport pathways from (sub)tropical source regions in…
Stratosphere–Troposphere Exchange and Its Relation to Potential Vorticity Streamers and Cutoffs near the Extratropical Tropopause
2007
Abstract Two distinct dynamical processes near the dynamical tropopause (2-PVU surface) and their relation are discussed in this study: stratosphere–troposphere exchange (STE) and the formation of distinct potential vorticity (PV) structures in the form of stratospheric and tropospheric streamers and cutoffs on isentropic surfaces. Two previously compiled climatologies based upon the 15-yr European Centre for Medium-Range Weather Forecasts Re-Analysis (ERA-15) dataset (from 1979 to 1993) are used to establish and quantify the link between STE and these PV structures. An event-based analysis reveals a strong relation between the two processes. For instance, on isentropes below 320 K, 30%–50%…
Resolution Dependence of the Tropopause Inversion Layer in an Idealized Model for Upper-Tropospheric Anticyclones
2009
Abstract This note investigates the dependence of the extratropical tropopause inversion layer (TIL) on numerical resolution in an idealized modeling framework. Axisymmetric upper-tropospheric anticyclones are constructed by specifying potential vorticity (PV) and solving the nonlinear PV-inversion problem. The PV distribution has a smooth but near-discontinuous change of PV across the tropopause in a transition zone with vertical depth δ. For fixed δ the strength of the TIL changes with changing resolution until the transition zone is resolved by a fairly large number of grid points. The quality-controlled numerical solutions are used to study the behavior for δ → 0. This limit can lead to…
Long-lived Rossby wave trains as precursors to strong winter cyclones over Europe
2013
The statistical connection between strong surface cyclones over Europe and long-lived upper-tropospheric Rossby wave trains is examined for the Northern Hemisphere winter season using 45 years of reanalysis data. Dates are selected for which the surface pressure anomaly over Central Europe is below a threshold yielding the 5% of lowest values. Composites of upper tropospheric meridional wind for these dates (including a lead or lag in time) display clear signs of a wave train. The composite wave train lives for over two weeks and propagates eastward over more than 360° longitude. The phase speed of individual lows and highs, by contrast, is close to zero and the same is true for the composi…
The ATAL within the 2017 Asian Monsoon Anticyclone: Microphysical aerosol properties derived from aircraft-borne in situ measurements
2021
Abstract. The Asian summer monsoon is an effective pathway for aerosol particles and precursor substances from the planetary boundary layer over Central, South, and East Asia into the upper troposphere and lower stratosphere. An enhancement of aerosol particles within the Asian monsoon anticyclone (AMA) has been observed by satellites, called the Asian Tropopause Aerosol Layer (ATAL). In this paper we discuss airborne in situ and remote sensing observations of aerosol microphysical properties conducted during the 2017 StratoClim field campaign within the region of the Asian monsoon anticyclone. The aerosol particle measurements aboard the high-altitude research aircraft M55 Geophysica (reac…
Th0 to Th1 switch of CD4 T cell clones specific from the 16-kDa antigen of Mycobacterium tuberculosis after successful therapy: lack of involvement o…
2005
Abstract In this study, we have examined the influence of HLA-DR molecules and the structure of the epitope repertoire of the 16-kDa protein of Mycobacterium tuberculosis on the acquisition of the cytokine secretion pattern of CD4 T cell clones, obtained from tuberculous patients before and after anti-mycobacterial therapy. Our data indicate that TB patients have a predominant Th0 response against the 16-kDa protein and its epitopes and that healing, induced by anti-mycobacterial therapy, is associated with a shift toward a predominant Th1 phenotype. Moreover, both HLA-DR molecules restricting the clone specificity and the nature of the recognized epitope do not play any role in the generat…
MMP-10/stromelysin-2 promotes invasion of head and neck cancer.
2011
BackgroundPeriostin, IFN-induced transmembrane protein 1 (IFITM1) and Wingless-type MMTV integration site family, member 5B (Wnt-5b) were previously identified as the invasion promoted genes of head and neck squamous cell carcinoma (HNSCC) by comparing the gene expression profiles between parent and a highly invasive clone. We have previously reported that Periostin and IFITM1 promoted the invasion of HNSCC cells. Here we demonstrated that Wnt-5b overexpression promoted the invasion of HNSCC cells. Moreover, stromelysin-2 (matrix metalloproteinase-10; MMP-10) was identified as a common up-regulated gene among Periostin, IFITM1 and Wnt-5b overexpressing HNSCC cells by using microarray data s…
Adaptation of microphysical and chemical instrumentation to the airborne measuring platform Iljushin I1-18 ‘Cyclone’ and flight regime planning durin…
1997
Abstract In 1993 the joint Russian-German Research Project ‘Arctic Haze’ started (see the Introduction and editorial note of this issue, by Jaenicke, Khattatov, Jaeschke and Leiterer). Besides CAO, four German groups were involved. To the present three airborne measuring campaigns have been performed. In total 251 h of flight within altitudes up to 8.7 km were flown in the western and eastern part of the arctic leading to a comprehensive set of data of the northern arctic hemisphere. The measurements were conducted aboard the Russian research aircraft I1-18 ‘Cyclone’ which was used by CAO in numerous scientific projects mainly in the former USSR. This 4 engined turboprop aircraft is well eq…