Search results for "Clostridium perfringens"

showing 3 items of 13 documents

Antibacterial effect of the bioactive compound beauvericin produced by Fusarium proliferatum on solid medium of wheat.

2010

To obtain the bioactive compound beauvericin (BEA), Fusarium proliferatum CECT 20569 was grown on a solid medium of wheat, utilizing the technique of the solid state fermentation (SSF), being this mycotoxin purified by high performance liquid chromatography (HPLC) with a reverse phase semi-preparative column using as the mobile phase acetonitrile/water in gradient condition. The purity of the BEA was verified by analytical HPLC and liquid chromatography tandem mass spectrometry (LC/MS-MS). The pure fractions of BEA were utilized to determinate the antibiotic effects on several bacterial strains that are considered normally pathogens of the intestinal tract as: Escherichia coli, Enterococcus…

Spectrometry Mass Electrospray IonizationFusarium proliferatumToxicologymedicine.disease_causeHigh-performance liquid chromatographymycotoxinchemistry.chemical_compoundFusariumTandem Mass SpectrometryLiquid chromatography–mass spectrometryDepsipeptideswheatmedicineTriticumAntibacterial agentChromatographybiologybeauvericinfood and beveragesClostridium perfringensbiology.organism_classificationBeauvericinBioactive compoundAnti-Bacterial AgentschemistrySolid-state fermentationSpectrophotometry UltravioletChromatography Liquid
researchProduct

Enzyme molecular mechanism as a starting point to design new inhibitors: a theoretical study of O-GlcNAcase.

2011

O-Glycoprotein 2-acetamino-2-deoxy-β-d-glucopyranosidase (O-GlcNAcase) hydrolyzes O-linked 2-acetamido-2-deoxy-β-d-glucopyranoside (O-GlcNAc) residues from post-translationally modified serine/threonine residues of nucleocytoplasmic protein. The chemical process involves substrate-assisted catalysis, where two aspartate residues have been identified as the two key catalytic residues of O-GlcNAcase. In this report, the first step of the catalytic mechanism used by O-GlcNAcase involving substrate-assisted catalysis has been studied using a hybrid quantum mechanical/molecular mechanical (QM/MM) Molecular Dynamics (MD) calculations. The free energy profile shows that the formation of the oxazol…

chemistry.chemical_classificationProtonStereochemistryClostridium perfringensOxazolineMolecular Dynamics Simulationbeta-N-AcetylhexosaminidasesSurfaces Coatings and FilmsCatalysisSubstrate SpecificitySerinechemistry.chemical_compoundHydrolysisMolecular dynamicsEnzymechemistryMaterials ChemistryQuantum TheoryThermodynamicsPhysical and Theoretical ChemistryThreonineEnzyme InhibitorsOxazolesThe journal of physical chemistry. B
researchProduct

ChemInform Abstract: Polycondensed Nitrogen Heterocycles. Part 17. Isoxazolo(4,3-d)pyrazolo-(3,4-f)(1,2,3)triazepine. A New Ring System.

1988

The title compounds were prepared by nitration of compounds 2, reduction of the dinitro derivatives 4 and diazotization of the diamino derivatives 6 followed by an intramolecular coupling reaction. Compound 4a showed good activity against Salmonella cholerasuis and Clostridium perfringens bacteria.

chemistry.chemical_elementGeneral MedicineClostridium perfringensRing (chemistry)medicine.disease_causeNitrogenMedicinal chemistryCoupling reactionSalmonella cholerasuischemistry.chemical_compoundchemistryIntramolecular forceNitrationmedicineChemInform
researchProduct