Search results for "Coma"

pRb suppresses camptothecin-induced apoptosis in human osteosarcoma Saos-2 cells by inhibiting c-Jun N-terminal kinase

2001

AbstractThis paper studies the cytotoxic effect induced by the topoisomerase I inhibitor camptothecin in human osteosarcoma Saos-2 cells, which lack p53 and contain a non-functional form of the product of the retinoblastoma gene, pRb. Cytotoxicity induced by camptothecin was dose- and time-dependent; the treatment with 100 nM camptothecin reduced cell viability by 50% at 32 h and by 75% at 72 h of exposure. The cytotoxic effect was caused by apoptosis, as ascertained by morphological evidence, acridine orange-ethidium bromide staining and flow cytometric analysis. Apoptosis was accompanied by both the activation of caspase-3 and the fragmentation of poly(ADP-ribose) polymerase. Treatment wi…

Let-7d miRNA Shows Both Antioncogenic and Oncogenic Functions in Osteosarcoma-Derived 3AB-OS Cancer Stem Cells

2015

Osteosarcoma (OS), an aggressive highly invasive and metastatic bone-malignancy, shows therapy resistance and recurrence, two features that likely depend on cancer stem cells (CSCs), which hold both self-renewing and malignant potential. So, effective anticancer therapies against OS should specifically target and destroy CSCs. We previously found that the let-7d microRNA was downregulated in the 3AB-OS-CSCs, derived from the human OS-MG63 cells. Here, we aimed to assess whether let-7d modulation affected tumorigenic and stemness properties of these OS-CSCs. We found that let-7d-overexpression reduced cell proliferation by decreasing CCND2 and E2F2 cell-cycle-activators and increasing p21 an…

Induction of apoptosis in human osteosarcoma Saos-2 cells by the proteasome inhibitor MG132 and the protective effect of pRb

2003

Induction of apoptosis in human osteosarcoma Saos-2 cells by the proteasome inhibitor MG132 and the protective effect of pRb

Safety and efficacy of changing to the travoprost/timolol maleate fixed combination (DuoTrav) from prior mono- or adjunctive therapy.

2010

Norbert Pfeiffer1, Maria-Luise Scherzer2, Hubert Maier3, Sonja Schoelzel4, Mark C Jasek5, Jeanette A Stewart6, William C Stewart6 On behalf of the DuoTravMED study group1Johannes Gutenberg-Universität Mainz, Mainz, Germany; 2Regenstauf, Germany; 3Gerolzhofen, Germany; 4Institutes of Molecular Medicine, University of Freiburg, Department of Surgery, Freiburg, Germany; 5Alcon Laboratories, Inc., Fort Worth, TX, USA; 6PRN Pharmaceutical Research Network, LLC, Charleston, SC, USAPurpose: To assess the safety and efficacy of changing to the travoprost/timolol fixed combination (TTFC) from other mono- or adjunctive therapies.Patients and methods: A prospective, open-label, observational …

Tono oculare espessore oculare

2004

Uso della monoterapia con Bimatoprost nella clinica quotidiana

2011

Gli Autori riportano i risultati di uno studio multicentrico,prospettico, osservazionale condotto nel 2010 sull'uso nella pratica clinica della monoterapia con l'analogo prostanidico ,Bimatoprost 0.3 mg/ml, in pazienti con glaucoma cronico ad angolo aperto mai trattati in precedenza se non con farmaci betabloccanti.

La legislación más relevante en materia de adicciones

2005

El presente trabajo tiene como objetivo, exponer sucintamente, y acotada cronológicamente, la legislación más destacada en materia de adicción, con especial referencia al alcoholismo, tabaquismo y drogas. Igualmente se expondrán aquellos elementos comunes y principios que identifica la legislación estatal, y las legislacio­nes autonómicas en la materia.

Mediación en drogodependencias: reflexión práctica y ámbitos de aplicación

2012

La mediación es una disciplina emergente que se está implantando gradualmente en todos los ámbitos de la sociedad. La mediación trabaja desde la gestión positiva del confl icto, acercándolo a las partes y haciéndolas responsables de sus propias decisiones. Para ello, se sirve de la ayuda de una fi gura neutral e imparcial que dirige el proceso para conseguir que las partes lleguen a un acuerdo satisfactorio y benefi cioso para ambas. Este procedimiento de solución de confl ictos está avalado a nivel estatal por la Ley 5/2012, de 6 de julio, de mediación en asuntos civiles y mercantiles. Sin embargo, por su estructura y fl exibilidad, la mediación es una herramienta que puede instaurarse en …

Revista española de drogodependencias

2008

Antecedentes. Los trastornos por uso de sustancias (TUS) se pueden presentan en cualquier sociedad. En el pais y en sus instituciones educativas son escasos los estudios epidemiologicos. Esta situacion dificulta la compresion epidemiologica, su prevencion, planificacion y evaluacion.Objetivo. Determinar la prevalencia vital e incidencia del ultimo ano de TUS en estudiantes de la Universidad de Los Andes, Merida -Venezuela.Método. Estudio observacional transversal en estudiantes seleccionados aleatoriamente de tres campus universitarios. La muestra represento el 5% del universo: n=1.018 y se evaluo mediante un instrumento autoadministrado, anonimo y validado para Venezuela con denominaciones…

Insights of Tris(2-pyridylmethyl)amine as anti-tumor agent for osteosarcoma: experimental and in silico studies

2021

Abstract Osteosarcoma (OS) is a malignant bone tumor and its occurrence is associated with high levels of microRNAs (miRNAs) and critical protein sinvolved on intracellular signaling pathways. Cisplatin and Doxorubicin are employed on chemotherapy, but their use cause side effects and contributes to multidrug resistance. Since several tripodal amines have been studied as anticancer agents, tris(2-pyridylmethyl)amine (TPA) was investigated against osteosarcoma cells. Here we show that TPA exhibits activity against MG-63 and Saos-2 cells. Cyclic voltammetry results indicate an interaction between TPA and dsDNA, denoted by blocking of the proton-assisted reduction of the 2-pyridylmethyl moiety…