Search results for "Cuba."
showing 10 items of 340 documents
Study of the potential toxicity of enniatins A, A(1), B, B(1) by evaluation of duodenal and colonic bioavailability applying an in vitro method by Ca…
2010
Abstract The bioavailability of the minor Fusarium mycotoxins enniatins (ENs) utilizing an in vitro method which allows the simulation of the small and large intestine tracts has been studied. This method, based on the application of the Caco-2 cells grown alone or in symbiosis with several strains characteristics of the gastrointestinal tract, has permitted to simulate the duodenal and colonic intestinal compartments, respectively. The duodenal bioavailability expressed as absorption value after 4 h of exposure, ranged from 57.7 to 76.8% for EN A, from 68.8 to 70.2% for EN A1, from 65.0 to 67.0% for EN B, and from 62.2 to 65.1% for EN B1. Colonic bioavailability after 48 h of incubation ra…
Relationship between the Stages of Development of Fusarium moniliforme ATCC 38932 and Production of Fusarin C
2019
A study of the kinetics of fusarin C production by Fusarium moniliforme ATCC 38932, a known producer of fusarin C, was carried out. This strain was subcultured on an EG medium for an adequate sporulation, and a 4% inoculum was transferred to the 10% ICI N medium. The conditions for the production of fusarin C in this synthetic culture medium were optimized. The time-course study of fusarin C performed over 26 days with this strain showed three different developmental stages in which a maximum production of fusarin C was reached on the 8th day of incubation; thereafter this strain ceased growing exponentially and exhibited a sharp decrease of fusarin C from that moment on.
Cytotoxic effects of mycotoxin combinations in mammalian kidney cells
2011
The cytotoxicity of three Fusarium mycotoxins (beauvericin, deoxynivalenol and T-2 toxin) has been investigated using the NR assay, after 24, 48 and 72h of incubation. The IC(50) values ranged from 6.77 to 11.08, 3.30 to 10.00 and 0.004 to 0.005 for beauvericin, deoxynivalenol and T-2 toxin, respectively. Once the potential interaction has been detected, a quantitative assessment is necessary to ensure and characterize these interactions, that is, each mycotoxin contributes to the toxic effect in accord with its own potency. Combination of mycotoxins was determined in Vero cells after 24, 48 and 72h of exposure. Isobolograms and median effect method of Chou and Talalay were used to assess t…
66 In vitro comparison of six raw materials on gastric ecosystem activity and acidity
2021
International audience; The great biochemical variability of the raw materials used in horse feeds can induce changes in gastric fermentation, and therefore affect gastric health through the resulting acidity of the bacterial end products. This in vitro trial compared the effect of 6 raw materials (BA: barley; WB: wheat bran; OA: oats; SM: sunflower meal; SB: sugar beet pulp; AL: alfalfa – Table 1) commonly used in horse feeds on gastric bacterial fermentation and buffering capacity. In 3 horses fed a mixture of the 6 raw materials 3h before sampling, gastric chyme was collected via a nasogastric tube and mixed. Bottles containing 1mL of the mixed gastric chymes, 50mL dilution medium and 1.…
An unusual magnetic response in a π-stacked 66-dia net structure of [4 + 2] copper(II) cubane
2015
A phenoxo bridged antiferromagnetic copper(II) cubane features a π-stacked 66-dia net framework and creates long range ferromagnetic ordering, as evidenced from a coercivity maximum (∼2000 Oe) at 20 K with very unusual saturation magnetization.
Coscienza e potere. Narrazioni attraverso il mito
2009
Il libro, che prende spunto dal seminario Il mito come evento ciclico nella cronaca del quotidiano. Coscienza e potere (Palermo -17 ottobre 2008), mette a confronto le diverse letture del mito, individuandone le differenti forme di attualizzazione. I personaggi di Antigone e Ecuba rappresentano, in tal senso, figure emblematiche. Attraverso una prospettiva letteraria, socio-giuridica e comunicativa, viene analizzato il nesso che lega sapere e potere, il conflitto tra dimensione individuale e assetto giuridico, il rapporto tra soggettività e coscienza collettiva.
(R)-NODAGA-PSMA: A Versatile Precursor for Radiometal Labeling and Nuclear Imaging of PSMA-Positive Tumors
2015
Purpose The present study aims at developing and evaluating an urea-based prostate specific membrane antigen (PSMA) inhibitor suitable for labeling with 111In for SPECT and intraoperative applications as well as 68Ga and 64Cu for PET imaging. Methods The PSMA-based inhibitor-lysine-urea-glutamate-coupled to the spacer Phe-Phe-D-Lys(suberoyl) and functionalized with the enantiomerically pure prochelator (R)-1-(1-carboxy-3-carbotertbutoxypropyl)-4,7-carbotartbutoxymethyl)-1,4,7-triazacyclononane ((R)-NODAGA(tBu)3), to obtain (R)-NODAGA-Phe-Phe-D-Lys(suberoyl)-Lys-urea-Glu (CC34). CC34 was labeled with 111In, 68Ga and 64Cu. The radioconjugates were further evaluated in vitro and in vivo in LNC…
The second component of human complement: Use of glycosidases and glucosylation to distinguish the two forms
1988
Abstract The two forms of human plasma C2 that were described in the preceding report (1) were investigated for their functional and biochemical differences. Incubation with the neuraminidase (NAN'dase) of Clostridium perfringens at 37°C resulted in a four- to fivefold increase in the hemolytic activity of both forms. The increase in activity was different than the increase caused by treatment with iodine. The mechanism of increased activity of NAN'dase-treated C2 was the generation of increased molecules of activated C3 (C3b), resulting in more molecules of C5 binding to (C4b, 2a, 3b)n. Removal of N-acetyl-neuraminate from C2 did not alter its binding to a cationic exchanger. Nonenzymatic …
Cell wall mannoproteins during the population growth phases in Saccharomyces cerevisiae.
1987
Mannoproteins from cell walls of Saccharomyces cerevisiae synthesized at successive stages of the population growth cycle have been solubilized with Zymolyase and subsequently analyzed. The major change along the population cycle concerned a large size mannoprotein material; the size of the newly-synthesized molecules varied from 120,000–500,000 (mean of about 200,000) at early exponential phase to 250,000–350,000 (mean of about 300,000) at late exponential phase. These differences are due to modifications in the amount of N-glycosidically linked mannose residues, since the size of the peptide moiety was 90,000–100,000 at all growth stages and the level of O-glycosylation changed only sligh…
Effects of sublethal levels of tributyltin chloride on a new toxicity test organism,Liza saliens (osteichthyes, mugilidae): a histological study
2006
The histopathological effects of 10−7 and 10−9M tributyltin(IV)chloride,TBTCl, solutions on different Liza saliens organs have been studied by light microscope. The fish were sacrificed after 3–4 h incubation in 10−7M TBTCl solution or after 15 days incubation in 10−9M solution. The observed histopathological changes were dose- and time-dependent. The 10−7M TBTCl concentration resulted in major damage to the gill epithelium, indicating that TBTCl primarily interfered with the respiration, osmoregulation, acid balance and nitrogenous waste excretion processes. After incubation in 10−9M TBTCl solution the fish lived 20 or more days, but many of the organs were altered. Thymus atrophy, reduced…