Search results for "Cucumber"
showing 10 items of 30 documents
Histological Change in Cucumber Tissue and Cellulase Activity of Plectosphaerella melonis Strain 502
2022
In the last ten years, many countries around the world recorded a new disease of the Cucurbitaceae, the agent of which was P. melonis. The ability of P. melonis 502 to form intracellular mycelium in the epidermal and parenchymal tissues of roots was shown. Leading tissues (xylem and phloem) did not colonize, which indicates the impossibility of plant vessel clogging and shows the fungus’s biochemical effects on plants, which causes the process of pathogenesis. P. melonis 502 is able to develop in a wide range of pH values, while the pH-optimum is 8.5. P. melonis 502 is able to adjust the pH of the medium to the optimal value—8.5. We also showed that cellulase enzyme synthesis de…
Studies on Holothuriapolii (echinodermata) coelomocyte lysate II. Isolation of coelomocyte hemolysins
1988
The lytic activity of the Holothuria polii coelomocyte lysate resides in two electrophoretically distinct hemolysins identified as He1 and He2. He1 represents the calcium dependent, heat-labile component whereas He2 is calcium independent and heat-stable. The two hemolysins share serological identity. Both hemolysins appear as single protein molecules of 80KDa molecular weight by SDS-PAGE and transblotting analysis under non-reducing conditions. However under reducing conditions, they are doublets of 76 and 80KDa molecular weight. The hypothesis that the two hemolysins could be isoforms is discussed.
How does climate change affect a fishable resource? The case of the royal sea cucumber (Parastichopus regalis) in the central Mediterranean Sea
2022
Holothurians or sea cucumbers are key organisms in marine ecosystems that, by ingesting large quantities of sediments, provide important ecosystem services. Among them, Parastichopus regalis (Cuvier, 1817) is one of the living sea cucumbers in the Mediterranean actively fished for human consumption mainly in Spain, where it is considered a gastronomic delicacy. In the Strait of Sicily (central Mediterranean Sea), this species is not exploited for commercial use even if it is used as bait by longline fishery. P. regalis is frequently caught by bottom trawling and discarded at sea by fishers after catch, and because of its capacity to resist air exposition (at least in cold months), it is rea…
Engagement of the periesophageal ring during Holothuria polii response to erythrocyte injection.
2009
In Holothuria polii, the periesophageal ring is an important organ supplying spherule cells after stimulation with foreign material. In animals injected with formalinized sheep erythrocytes, in fact, a depletion of spherule cells is observed in the periesophageal ring, whereas in the connective tissue, in the external epithelium and around the antigen- injected site, small, transparent cells can be visualized. It is supposed that the latter are stem cells of spherule cells.
Cucumis sativus l. Seeds ameliorate muscular spasm-induced gastrointestinal and respiratory disorders by simultaneously inhibiting calcium mediated s…
2021
Cucumis sativus L. is globally cultivated as an edible vegetable. Besides its nutritional benefits, it is used in traditional medicines against various ailments. The current study was designed to elucidate the multi-target mechanisms of a C. sativus seeds extract against asthma and diarrhea using network pharmacology along with a molecular docking approach. Furthermore, in-vitro and in-vivo experiments were conducted to verify the mechanistic insight of in silico studies. LC-ESI-MS/MS was performed to identify the bioactive compounds in the extract; later, some compounds were quantified by HPLC. C. sativus seed. EtOH has kaempferol in higher concentration 783.02 µg/g, followed by quercetin …
Antimicrobial peptides from echinoderms as antibiofilm agents: a natural strategy to combat bacterial infections
2014
AbstractIncreased attention has been focused on marine invertebrates as a source of bioactive molecules for biomedical applications. Many bioactive molecules are part of the innate immune system. Some more recently isolated compounds, mainly from the sea urchin and the sea cucumber, are antimicrobial peptides (AMPs) active against Gram-positive and Gram-negative bacteria, and fungi. In this review we described the most recent studies on AMPs isolated from echinoderms. AMPs are small peptides (< 10 kDa) with cationic charge and amphipathic structure. Recently, it was demonstrated that in the coelomocyte lysates of Paracentrotus lividus and Holothuria tubulosa, AMPs possess activity against s…
Alternating current voltammetric determination of DNA damage
1990
Abstract The conditions for alternating current (a.c.) voltammetric DNA determinations have been investigated with respect to its use with alkaline filter elution techniques at low DNA concentrations. In inorganic electrolyte solutions three current peaks can be distinguished: peak I around −1.1 V caused by the reorientation or desorption of DNA segments; peak II around −1.2 V caused by the native DNA (nDNA) form; peak III caused by denatured DNA (dDNA) at −1.4 V. Sonication of nDNA increases the peak current, however not with dDNA. Both dDNA and nDNA give linear peak current increments with DNA increments, their regression lines cutting the concentration axis at the origin. In filter eluti…
Population genetics of cucumber mosaic virus infecting medicinal, aromatic and ornamental plants from northern Italy
2012
An epidemiological survey carried at the Herb Garden of Casola Valsenio (Emilia Romagna region; Northern Italy) from 2006 to 2009 resulted in finding Cucumber mosaic virus (CMV) as the infectious agent of several ornamental, medicinal and aromatic plants. All CMV-infected plant species showed leaf symptoms followed, in some cases, by flower colour-breaking and stunting. Genetic analysis consisted in the characterization of movement protein gene of each CMV-isolate by single strand polymorphism analysis and sequencing. Phylogenetic trees identified only two isolates as member of subgroup II, while all other isolates were part of subgroup IA. This survey confirms an unexpected increment of CM…
Characterization of a Cucumber mosaic virus isolate infecting Mandevilla sanderi (Hemsl.) Woodson
2005
In 2004, virus-like symptoms consisting of yellow rings in older leaves were observed in the foliage of Mandevilla sanderi grown from shoots in the province of Catania (Sicily, Italy). The plants were tested for virus presence using DAS-ELISA, mechanical inoculations to the host, RT-PCR and SSCP. Serological tests detected Cucumber mosaic virus (CMV) in all symptomatic leaf samples. The virus was mechanically transmitted to 11 of the 15 host species tested, including some of the Chenopodiaceae and Solanaceae families; indicator plants showed symptoms typical of CMV subgroup I. RT-PCR, SSCP and cloning were employed to characterize this CMV isolate, and to make clear its genetic relationship…
Humoral responses during wound healing in Holothuria tubulosa (Gmelin, 1788)
2021
Abstract Wounds in living organisms trigger tissue-repair mechanisms. The sea cucumber (Holoturia tubulosa) is an excellent model species for achieving a better understanding of the humoral and cellular aspects involved in such healing processes. Consequently, this study assesses data on its morphometric, physiological and humoral responses 1, 2, 6, 24 and 48h after wound induction. In particular, morphometric data on the weight, width, length and coelomic-fluid volume of the species were estimated at different times during our experiments. In addition, the humoral aspects related to the enzymatic activity of esterase, alkaline phosphatase and peroxidase, as well as the cytotoxic activity o…