Search results for "Cytochrome"

An update of the known distribution and status of Cherax spp. in Italy (Crustacea, Parastacidae)

2022

To date, only two Cherax species have been reported to occur in Italy, i.e., C. destructor Clark, 1936 and C. quadricarinatus (von Martens, 1868), both in the wild and in aquaculture farms. Therefore, we aimed to update their current status and distribution in Italian mainland and Sicily. In addition, we investigated the origin of their known populations, and their possible routes of invasion. In order to genetically characterize the Cherax populations occurring in Italian inland waters and aquaculture facilities, the barcode region of the mtDNA gene cytochrome oxidase subunit I was sequenced in the available specimens originating from an aquaculture facility and a museum collection. The sa…

An in vitro tool to assess cytochrome P450 drug biotransformation-dependent cytotoxicity in engineered HepG2 cells generated by using adenoviral vect…

2011

Many adverse drug reactions leading to hepatotoxicity are caused by the cytochrome P450-dependent activation of non-toxic drugs or chemicals into reactive metabolites. To this end, adenoviruses were used as a tool to efficiently deliver specific CYP genes into cultured cells (i.e., human hepatoma cell line HepG2). Recombinant-defective adenoviral vectors encoding for genes CYP3A4 (Adv-CYP3A4), CYP2E1 (Adv-CYP2E1), CYP2A6 (Adv-CYP2A6) and CYP1A2 (Adv-CYP1A2) were used to confer specific CYP drug metabolic capabilities to HepG2 cells. Upgraded cells transiently expressed single specific cytochrome P450 enzymatic activities in terms of the number of the infecting virus particles used in their …

Assessing the use of the mitochondrial cox1 marker for use in DNA barcoding of red algae (Rhodophyta)

2011

The red algae, a remarkably diverse group of organisms, are difficult to identify using morphology alone. Following the proposal to use the mitochondrial cytochrome c oxidase subunit 1 (cox1) for DNA barcoding animals, we assessed the use of this, ne in the identification of red algae using 48 samples plus 31 sequences obtained from GenBank. The data set spanned six gene orders of red algae: the Bangiales, Ceramiales, Corallinales, Gigartinales, Gracilariales and Rhodymeniales. The results indicated that species could be discriminated. Intraspecific variation was between 0 and 4 bp over 539 bp analyzed except in Mastocarpus stellatus (0-14 bp) and Gracilaria gracilis (0-11 bp). Cryptic dive…

The musculature of the electric fish Eigenmannia virescens (South American green knife fish) characterized with cytochrome oxidase staining

1986

Using cytochrome C-oxidase staining, different types of somatic musculature were clearly distinguished in the gymnotoid fish Eigenmannia virescens. Except for a few thin fibres in the region of the horizontal septum, which stained faintly, no others in the trunk muscle stained. Strong staining appeared in the fibres of the anal fin muscles. According to the classification of fish musculature into white, intermediate and red, only the locomotory organ of this fish has red fibres, whereas the trunk muscles are white. The red muscles along the horizontal septum, found in all other fish which have been investigated in this respect, seem to be absent. This is noteworthy since the anal fin alone …

Cytochrome oxidase activity in the mitochondria of unfertilized and fertilized sea urchin eggs

1959

Abstract The cytochrome oxidase activity of isolated mitochondria of unfertilized and fertilized eggs of Paracentrotus lividus has been studied. It has been found that whereas in the mitochondria of unfertilized eggs saturation is reached at a cytochrome c concentration of 3.28 × 10−5 M, in those of fertilized eggs this occurs at a concentration of 5.74 × 10−5 M. It is shown that upon fertilization an increase of about 30 per cent of the activity of cytochrome oxidase takes place and no further changes appear to occur until blastula stage.

2019

The olfactory epithelium is continuously exposed to exogenous chemicals, including odorants. During the past decade, the enzymes surrounding the olfactory receptors have been shown to make an important contribution to the process of olfaction. Mammalian xenobiotic metabolizing enzymes, such as cytochrome P450, esterases and glutathione transferases (GSTs), have been shown to participate in odorant clearance from the olfactory receptor environment, consequently contributing to the maintenance of sensitivity toward odorants. GSTs have previously been shown to be involved in numerous physiological processes, including detoxification, steroid hormone biosynthesis, and amino acid catabolism. The…

The mechanism of the antioxidant effect of smoked paprika from La Vera, Spain

2013

This study aimed at further investigating the antioxidant effect of smoked paprika (Pimenton La Vera, Spain) in healthy subjects. Crossover study included male students (age 20.6 ± 1.7 years) who consumed smoked or non-smoked paprika (2 g/day, 20 times, over 30 days). After ingestion of smoked paprika, plasma malondialdehyde (MDA) levels were significantly (p < 0.01) reduced versus basal and non-smoked paprika ingestion values. Reduced glutathione (GSH) levels were increased and oxidised glutathione (GSSG) levels reduced significantly (p < 0.05) in paired subjects after consumption of smoked paprika. Smoked paprika significantly increased mRNA levels of glutathione peroxidase and cytochrome…

Action of low doses of Aspirin in Inflammation and Oxidative Stress induced by aβ1-42 on Astrocytes in primary culture

2020

Aspirin has been used as anti-inflammatory and anti-aggregate for decades but the precise mechanism(s) of action after the presence of the toxic peptide Aβ1-42 in cultured astrocytes remains poorly resolved. Here we use low-doses of aspirin (10-7 M) in astrocytes in primary culture in presence or absence of Aβ1-42 toxic peptide. We noted an increase of cell viability and proliferation with or without Aβ1-42 peptide presence in aspirin treated cells. In addition, a decrease in apoptosis, determined by Caspase 3 activity and the expression of Cyt c and Smac/Diablo, were detected. Also, aspirin diminished necrosis process (LDH levels), pro-inflammatory mediators (IL-β and TNF-α) and NF-ᴋB prot…

Encapsulation of cytochrome C by multilayer microcapsules. A model for improved enzyme immobilization

1997

Cytochrome C was used as a model compound to study the behavior of proteins during the encapsulation procedure in alginate microcapsules and the loss by diffusion during storage. Alginate gel beads were coated with a multilayer membrane consisting of alternating layers of poly(N-vinylamine) and poly(acrylic acid) or water soluble anionic cellulose derivatives, respectively. To obtain high encapsulation yields, it is recommended to use the lowest possible concentration of salts in the precipitation bath. Loss during storage (and use) is minimized by applying at least two polycation/polyanion double layers for the membrane. Cellulose derivatives are superior over poly(acrylic acid). Single la…

Luminometric sub-nanoliter droplet-to-droplet array (LUMDA) and its application to drug screening by phase I metabolism enzymes.

2012

Here we show the fabrication of the Luminometric Sub-nanoliter Droplet-to-droplet Array (LUMDA chip) by inkjet printing. The chip is easy to be implemented and allows for a multiplexed multi-step biochemical assay in sub-nanoliter liquid spots. This concept is here applied to the integral membrane enzyme CYP3A4, i.e. the most relevant enzymatic target for phase I drug metabolism, and to some structurally-related inhibitors.