Search results for "DAMAGE"
showing 10 items of 1289 documents
Farbenwandel der Zahnhartgewebe als Zeichen thermischer Sch�digung
1985
Die menschlichen Hartgewebe lassen nach thermischer Schadigung gewisse Ruckschlusse auf Brandparameter zu. Neben morphologischer Destruktion ist an den Zahnhartgeweben ein Farbenwandel zu beobachten. Diese Form der Zustandsanderung von Schmelz, Dentin und Zement ist an 330 menschlichen extrahierten Zahnen unter definierten Zeit-und Temperaturintervallen studiert worden. Die Farbphanomene als Ergebnisse der Gluh- und Aufheizversuche sind mit Hilfe der DIN- und RAL-Farbregister ausgewertet worden. Am leichtesten last sich die Zementfarbe bestimmen, da markierende Belage oder Konkremente fehlen. Auserdem bleibt die Wurzelform relativ lange erhalten, wahrend die Zahnkronen zerfallen konnen. Die…
Consensus guidelines for the detection of immunogenic cell death
2014
Apoptotic cells have long been considered as intrinsically tolerogenic or unable to elicit immune responses specific for dead cell-associated antigens. However, multiple stimuli can trigger a functionally peculiar type of apoptotic demise that does not go unnoticed by the adaptive arm of the immune system, which we named "immunogenic cell death" (ICD). ICD is preceded or accompanied by the emission of a series of immunostimulatory damage-associated molecular patterns (DAMPs) in a precise spatiotemporal configuration. Several anticancer agents that have been successfully employed in the clinic for decades, including various chemotherapeutics and radiotherapy, can elicit ICD. Moreover, defect…
Topoisomerase II inhibition and high yield of endoreduplication induced by the flavonoids luteolin and quercetin
2006
Luteolin and quercetin are widely distributed plant flavonoids that possess a variety of chemical and biological activities, including free-radical scavenging and antioxidant activity. Recently, both flavonoids have been reported to inhibit DNA topoisomerases I and II (topo I and topo II), a property that, together with their ability to induce DNA and chromosome damage, has made them candidate anticancer compounds. In the present study, we confirmed that both compounds are topo II inhibitors by conducting a comparative study of their effect on topo II activity from Chinese hamster ovary AA8 cells. Because interference with the function of topo II to resolve DNA entanglement at the end of re…
Study of the Mode and Efficiency of DNA Binding in the Damage Induced by Photoactivated Water Soluble Porphyrins
2013
We have investigated the DNA binding interactions and in vitro photoactivated DNA damage induced by a neutral water soluble porphyrin derivative 5,10,15,20-tetrakis(2,4,6-trihydroxyphenyl)porphyrin (TTHPP) and its zinc derivative 5,10,15,20-tetrakis(2,4,6-trihydroxyphenyl)porphyrinato zinc(II) (Zn-TTHPP) upon visible light irradiation through various spectroscopic techniques and employing repair endonucleases. These porphyrin derivatives exhibited high affinity toward DNA through groove binding interactions as evidenced through the UV-vis absorption, emission, circular dichroism spectral and viscosity changes. Interestingly, the free base porphyrin derivative, TTHPP generated efficient sing…
Effect of Stem Snapping on Aspen Timber Assortment Recovery in Hemiboreal Forests
2020
Post-disturbance salvage logging mitigates economic loss after windthrow, and the value of salvaged timber is strongly linked to its quality and dimensions. We studied the occurrence of wind-induced damage of aspen in the hemiboreal forests of Latvia based on data from the National Forest Inventory and additional measurements. Individual tree data from three re-measurement periods were linked to follow a tree condition (live, broken, uprooted) and to link tree characteristics to a respective snag. Three linear models were developed to assess factors affecting the snapping height. An assortment outcome was calculated for undamaged and salvaged trees using the bucking algorithm, and timber va…
A modified Ant Colony damage identification algorithm for not well spaced frequency systems
2012
Damage identification is of primary concern in many fields of civil engineering. Usually the damage is detected from the variation of structural response induced. When the damage level is very low, incipient damage, this variation is hardly seen. In the present work is studied the case of not well spaced frequency systems. Identification problem is formulated as a minimum problem of a functional expressed in term of damage parameters. The minimum problem is solved by heuristic algorithm, ACORL.
Multi-layered chromatin proteomics identifies cell vulnerabilities in DNA repair
2023
Abstract The DNA damage response (DDR) is essential to maintain genome stability, and its deregulation predisposes to carcinogenesis while encompassing attractive targets for cancer therapy. Chromatin governs the DDR via the concerted interplay among different layers, including DNA, histone post-translational modifications (hPTMs) and chromatin-associated proteins. Here, we employ multi-layered proteomics to characterize chromatin-mediated functional interactions of repair proteins, signatures of hPTMs and the DNA-bound proteome during DNA double-strand break (DSB) repair at high temporal resolution. Our data illuminate the dynamics of known and novel DDR-associated factors both at chromati…
B Cell Expansion Hinders the Stroma-Epithelium Regenerative Crosstalk During Mucosal Healing
2021
Little is known about the pro-resolution role of immune cells recruited to damaged tissue. Using an experimental model of intestinal epithelial damage and repair, we identified B cells as the dominant cell type in the healing colon. Single-cell RNA-sequencing (scRNAseq) revealed the expansion of an IFN-induced B cell subset during experimental mucosal healing which was associated with colitis severity. In line with this, B cell depletion during mucosal healing resulted in accelerated recovery upon injury, which was associated with enhanced expression of tissue remodeling genes. scRNA-seq from the epithelial and stromal compartment confirmed that lack of B cells during mucosal healing alters…
Zfp819, a novel KRAB-zinc finger protein, interacts with KAP1 and functions in genomic integrity maintenance of mouse embryonic stem cells
2013
AbstractPluripotency is maintained by both known and unknown transcriptional regulatory networks. In the present study, we have identified Zfp819, a KRAB-zinc finger protein, as a novel pluripotency-related factor and characterized its role in pluripotent stem cells. We show that Zfp819 is expressed highly in various types of pluripotent stem cells but not in their differentiated counterparts. We identified the presence of non-canonical nuclear localization signals in particular zinc finger motifs and identified them as responsible for the nuclear localization of Zfp819. Analysis of the Zfp819 promoter region revealed the presence of a transcriptionally active chromatin signature. Moreover,…
Homozygous familial hypercholesterolaemia: new insights and guidance for clinicians to improve detection and clinical management. A position paper fr…
2014
Item does not contain fulltext AIMS: Homozygous familial hypercholesterolaemia (HoFH) is a rare life-threatening condition characterized by markedly elevated circulating levels of low-density lipoprotein cholesterol (LDL-C) and accelerated, premature atherosclerotic cardiovascular disease (ACVD). Given recent insights into the heterogeneity of genetic defects and clinical phenotype of HoFH, and the availability of new therapeutic options, this Consensus Panel on Familial Hypercholesterolaemia of the European Atherosclerosis Society (EAS) critically reviewed available data with the aim of providing clinical guidance for the recognition and management of HoFH. METHODS AND RESULTS: Early diagn…