Search results for "DAS"
showing 10 items of 4164 documents
Filter paper disk techniques for assay of nucleotidase
1977
A DE filter disk technique for assaying the activity of nucleotidase is described. This method is based on the observation that nucleotides bind to the filters at 5 mM Tris-HCl (pH 7.8) while nucleosides do not. As parameter for the nucleotidase activity the decrease of bound nucleotides is determined. In parallel experiments the amount of the product (nucleoside) formed can be measured by DEAE Sephadex column chromatography. The filter disk technique can be applied for the determination of vmax and Km of a nucleotidase by using different ribonucleosidase monophosphate substrates.
Promoção da saúde: O renascimento de uma ideologia
2004
A concepção actual de promoção de saúde, educação para a saúde e prevenção da doença é suportada por ideologias algo adulteradas pela realidade social. Tendo em conta a proliferação epistemológica destes conceitos no final do século XX pretende-se, no presente artigo, elaborar uma reflexão crítica em torno das múltiplas racionalidades estratégicas em que os conceitos se organizam, bem como, abordar os paradoxos que se instalam ao nível dos determinantes biopsicossociais dos comportamentos salutogénicos. Partilhando responsabilidades, os profissionais de saúde, devem, colectiva e proactivamente, desempenhar um papel significativo como promotores de saúde, modelando e reforçando o significado…
Ilgtspējīgas attīstības aspektu integrēšana dabaszinību stundās vidusskolā
2018
Maģistra darba “Ilgtspējīgas attīstības aspektu integrēšana dabaszinību stundās vidusskolā” aktualitāti nosaka mūsdienu globālās problēmas, kas saistītas ar atmosfēras piesārņojumu un globālo sasilšanu, kā arī pārmērīgu resursu izmantošanu. Pētījuma mērķis ir izpētīt, kā integrēt ilgtspējīgas attīstības aspektus tēmas “Enerģija” apguvei dabaszinībās 12. klasei, skolēnu paradumu maiņas un zināšanu pilnveidei. Pētījumā iesaistījās Rīgas Valsts 3. ģimnāzijas 46 skolēni un četri fizikas skolotāji. Pētījuma ietvaros darba autore ir izstrādājusi tematisko plānu, nodarbību plānus ilgtspējīgas enerģijas aspektu integrēšanai mācību saturā, kas veidoti pamatojoties uz normatīvajiem dokumentiem, kā ar…
Heterogeneity of Stem Cells in the Thyroid
2019
Identification of thyroid stem cells in the past few years has made important contributions to our understanding of the cellular and molecular mechanisms that induce tissue regeneration and repair. Embryonic stem (ES) cells and induced-pluripotent stem cells have been used to establish reliable protocols to obtain mature thyrocytes and functional follicles for the treatment of thyroid diseases in mice. In addition, the discovery of resident thyroid progenitor cells, along with other sources of stem cells, has defined in detail the mechanisms responsible for tissue repair upon moderate or severe organ injury.In this chapter, we highlight in detail the current state of research on thyroid ste…
Plano del real sitio del Pardo y Viñuélas
Orientat amb fletxa
Arte mueble de la España mediterránea : Breve síntesis y algunas consideraciones teóricas
1994
En este trabajo se analiza el arte mueble de/Mediterráneo español, basándose especialmente en la colección de plaquetas de El Parpalló. El estudio detallado de los motivos artísticos y su correlación estratigráfica revela algunas diferencias cronológicas en un marco de evolución continua. Conceptos tradicionales, como el de “Provincia Mediterránea‘~ son revisados a la luz de nuevos enfoques teóricosy empíricos. Portable art of Mediterranean Spain is analyzed in this paper with special reference (o the more than five thousand decoratedplaquettesfrom El Parpalló. A detailed study of artistic mot¡fs and stratigraphy reveals some chronological differences inside a frame of continous evolution. …
Late-Onset Globoid Cell Leukodystrophy: Unusual Ultrastructural Pathology and Subtotal β-Galactocerebrosidase Deficiency
1990
An 11-year-old girl was found to have severely reduced β-galactocerebrosidase activity as evidence of late-onset globoid cell leukodystrophy, while her mother had almost normal enzyme activity in circulating white blood cells. Clinically, the patient showed a remitting course marked by seizures, ataxia, white-matter disease on computed tomographic scan, and reduced conduction velocities of peripheral nerves. Symptoms improved somewhat around the age of 10 years. Two sural nerve biopsies, performed 6 years apart, disclosed a demyelinating neuropathy. By electron microscopy, membrane-bound vacuolar lysosomes in Schwann cells of myelinated axons, unlike the typical needlelike inclusions seen …
Early development of human Merkel cells.
1992
Human fetal Merkel cells are now generally considered to be epidermal derivatives. Previous studies using antibodies against the simple epithelial cytokeratins (CKs), 8 and 18, have demonstrated the presence of these cells in the epidermis at as early as fetal week 10 to 12. Using antibodies against CK 20 whose expression within the skin is restricted to Merkel cells, we applied immunofluorescence and immunoperoxidase microscopy to analyze earlier embryonic and fetal human skin (wk 7 to 9). We were able to demonstrate the first Merkel cells at as early as fetal wk 8, i.e., at the same time as the epidermis starts to develop an intermediate, third layer, characterized by the expression of CK…
Early access experience with VPRIV®: Recommendations for ‘core data’ collection
2011
Novel alpha-galactosidase A mutation in a female with recurrent strokes.
2012
Abstract Anderson–Fabry disease (AFD) is an X-linked inborn error of glycosphingolipid catabolism resulting from the deficient activity of the lysosomal exoglycohydrolase, a-galactosidase A. The complete genomic and cDNA sequences of the human alpha-galactosidase A gene have been determined and to date, several disease-causing alpha-galactosidase A mutations have been identified, including missense mutations, small deletions/insertions, splice mutations, and large gene rearrangements We report a case of a 56-year-old woman with recurrent cryptogenic strokes. Ophthalmological examination revealed whorled opacities of the cornea (cornea verticillata) and dilated tortuous conjunctival vessels.…