Search results for "DIF"

Differentiation in CLIL : methods and challenges

2014

CLIL (Content and Language Integrated Learning) -opetus eli vieraskielinen opetus tähtää sujuvaan kommunikaatioon ja monipuoliseen kielenkäyttöön ja tuo mukanaan uudenlaisia haasteita eriyttämisen saralle. CLIL on kohtalaisen uusi metodi Suomessa, eikä yksittäisiä opetuksen osa-alueita kuten eriyttämistä ole tutkittu sen osalta kovinkaan laajalti. Eriyttäminen antaa jokaisella oppilaalle mahdollisuuden kehittyä omalla lähikehityksen vyöhykkeellään ja siksi eriyttämistä on tärkeää tutkia myös vieraan kielen kannalta CLIL-opetuksessa. Tutkimuksen tavoitteena oli selvittää, minkälaisissa tilanteissa ja mitä keinoja käyttäen alakoulutason CLIL-opettajat eriyttävät omassa opetuksessaan ja mitkä …

Tecnica dell'acidificazione dei vini

2008

El destino natural de las mujeres. La legitimación de la violencia de género a través de la prensa sensacionalista del franquismo

2013

La prensa sensacionalista del franquismo colaboró activamente en la pres-cripción de un único modelo de mujer, el de madre y esposa confinada al hogar. El Estado promulgaba leyes que impedían trabajar a las mujeres, la Iglesia imponía sus preceptos morales y la educación segregaba los currículos de las niñas. A su vez, los medios de comunicación trasladaban a la opinión pública la teoría de la diferencia sexual. Las mujeres fueron usadas como pie-za clave de la política social y económica del régimen y, aunque a lo largo de las décadas cambiaron sus condiciones de vida, el discurso esencialista sobre la “condición femenina” permaneció intacto. El Caso, el semanario de sucesos de más éxito d…

High-Reynolds-number turbulent cavity flow using the lattice Boltzmann method

2018

We present a boundary condition scheme for the lattice Boltzmann method that has significantly improved stability for modeling turbulent flows while maintaining excellent parallel scalability. Simulations of a three-dimensional lid-driven cavity flow are found to be stable up to the unprecedented Reynolds number $\mathrm{Re}=5\ifmmode\times\else\texttimes\fi{}{10}^{4}$ for this setup. Excellent agreement with energy balance equations, computational and experimental results are shown. We quantify rises in the production of turbulence and turbulent drag, and determine peak locations of turbulent production.

Diffusion of two different isolates of Citrus tristeza virus in Sicily

2007

Osservation on the rapid spread of Citrus tristeza virus severe isolate by Aphis gossypii Glover.

2007

Transfection of lipoma cells with papilloma bovine virus subgenomic fragment.

1991

Abstract Lipoma cells with consistent chromosomal aberration have been transfected with plasmids carrying papilloma bovine virus subgenomic fragment (PBV 69). The succesful transformation of the cells was ascerted on the changed growth pattern of the cells in liquid medium, colony formation in soft agar and modified cell appearrance in electron microscopy; transfection with PBV 69 has not been, however, sufficient to immortalize lipoma cells.

CVB3 VP1 interacts with MAT1 to inhibit cell proliferation by interfering with Cdk-activating kinase complex activity in CVB3-induced acute pancreati…

2021

Coxsackievirus B3 (CVB3) belongs to the genus Enterovirus of the family Picornaviridae and can cause acute acinar pancreatitis in adults. However, the molecular mechanisms of pathogenesis underlying CVB3-induced acute pancreatitis have remained unclear. In this study, we discovered that CVB3 capsid protein VP1 inhibited pancreatic cell proliferation and exerted strong cytopathic effects on HPAC cells. Through yeast two-hybrid, co-immunoprecipitation, and confocal microscopy, we show that Menage a trois 1 (MAT1), a subunit of the Cdk-Activating Kinase (CAK) complex involved in cell proliferation and transcription, is a novel interaction protein with CVB3 VP1. Moreover, CVB3 VP1 inhibited MAT…

Infection-induced chromatin modifications facilitate translocation of herpes simplex virus capsids to the inner nuclear membrane

2021

Herpes simplex virus capsids are assembled and packaged in the nucleus and move by diffusion through the nucleoplasm to the nuclear envelope for egress. Analyzing their motion provides conclusions not only on capsid transport but also on the properties of the nuclear environment during infection. We utilized live-cell imaging and single-particle tracking to characterize capsid motion relative to the host chromatin. The data indicate that as the chromatin was marginalized toward the nuclear envelope it presented a restrictive barrier to the capsids. However, later in infection this barrier became more permissive and the probability of capsids to enter the chromatin increased. Thus, although …

Single-chain antibodies against a plant viral RNA-dependent RNA polymerase confer virus resistance.

2004

Crop loss due to viral diseases is still a major problem for agriculture today. We present a strategy to achieve virus resistance based on the expression of single-chain Fv fragments (scFvs) against a conserved domain in a plant viral RNA-dependent RNA polymerase (RdRp), a key enzyme in virus replication. The selected scFvs inhibited complementary RNA synthesis of different plant virus RdRps in vitro and virus replication in planta. Moreover, the scFvs also bound to the RdRp of the distantly related hepatitis C virus. T(1) and T(2) progeny of transgenic lines of Nicotiana benthamiana expressing different scFvs either in the cytosol or in the endoplasmic reticulum showed varying degrees of r…