Search results for "DISCOVERY"

showing 10 items of 4119 documents

Recent advances in computational design of potent aromatase inhibitors: open-eye on endocrine-resistant breast cancers.

2019

Introduction: The vast majority of breast cancers (BC) are estrogen receptor positive (ER+). The most effective treatments to fight this BC type rely on estrogen deprivation therapy, by inhibiting the aromatase enzyme, which performs estrogen biosynthesis, or on blocking the estrogens signaling path via modulating/degrading the estrogen's specific nuclear receptor (estrogen receptor-?, ER?). While being effective at early disease stage, patients treated with aromatase inhibitors (AIs) may acquire resistance and often relapse after prolonged therapies. Areas covered: In this compendium, after an overview of the historical development of the AIs currently in clinical use, and of the computati…

Antineoplastic Agents Hormonalmedicine.drug_classCYP450sEstrogen receptorallostery; aromatase inhibitors; Breast cancer; CYP450s; ligand-based and structure-based drug design; molecular dynamics; virtual screeningBreast NeoplasmsMolecular Dynamics SimulationBioinformatics03 medical and health sciencesBreast cancer0302 clinical medicineBreast cancerDrug DiscoverymedicineEndocrine systemHumansAromataseSurvival rate030304 developmental biologyCause of deathNeoplasm Staging0303 health sciencesallosterybiologybusiness.industryAromatase Inhibitorsvirtual screeningmedicine.diseaseligand-based and structure-based drug designmolecular dynamicsSurvival RateNuclear receptorEstrogenDrug Resistance Neoplasm030220 oncology & carcinogenesisDrug Designbiology.proteinFemalebusinessExpert opinion on drug discovery
researchProduct

Toward a Rational Design of Polyamine-Based Zinc-Chelating Agents for Cancer Therapies.

2020

In vitro viability assays against a representative panel of human cancer cell lines revealed that polyamines L1a and L5a displayed remarkable activity with IC50 values in the micromolar range. Preliminary research indicated that both compounds promoted G1 cell cycle arrest followed by cellular senescence and apoptosis. The induction of apoptotic cell death involved loss of mitochondrial outer membrane permeability and activation of caspases 3/7. Interestingly, L1a and L5a failed to activate cellular DNA damage response. The high intracellular zinc-chelating capacity of both compounds, deduced from the metal-specific Zinquin assay and ZnL2+ stability constant values in solution, strongly sup…

Antineoplastic AgentsApoptosis01 natural sciences03 medical and health scienceschemistry.chemical_compoundStructure-Activity RelationshipCell Line TumorDrug DiscoveryPolyaminesHumansCytotoxicityCaspase030304 developmental biologyChelating Agents0303 health sciencesbiologyMolecular StructureChemistryRational designG1 Phase Cell Cycle Checkpoints0104 chemical sciencesCell biology010404 medicinal & biomolecular chemistryZincModels ChemicalApoptosisCell cultureDrug Designbiology.proteinMolecular MedicineQuantum TheoryDrug Screening Assays AntitumorPolyamineG1 phaseIntracellularJournal of medicinal chemistry
researchProduct

Identification of Biphenyl-Based Hybrid Molecules Able To Decrease the Intracellular Level of Bcl-2 Protein in Bcl-2 Overexpressing Leukemia Cells

2009

With the aim of enhancing the structural complexity and diversity of an existing collection of bi- and terphenyl compounds, we synthesized hybrid molecules comprising of spirocyclic ketones (a complexity-bearing core) and bi/terphenyls (privileged fragments). Compounds 1, 3, 4, and 6 showed well-defined activity on apoptosis and differentiation, making them potential leads for development as new anticancer agents and chemical probes to study signaling networks in neoplastic cells.

Antineoplastic AgentsApoptosisHL-60 CellsChemical synthesisStructure-Activity RelationshipLeukemia Myelogenous Chronic BCR-ABL PositiveTerphenyl CompoundsDrug DiscoverymedicineHumansSpiro CompoundsChemistryBiphenyl CompoundsCell DifferentiationBiological activityKetonesmedicine.diseaseIn vitroLeukemiaProto-Oncogene Proteins c-bcl-2BiochemistryApoptosisCell cultureMolecular MedicineTerphenyl CompoundsK562 CellsIntracellularJournal of Medicinal Chemistry
researchProduct

Antimicrobial and antiproliferative activity of Peucedanum nebrodense (Guss.) Strohl.

2003

Abstract Acetone extract of Peucedanum nebrodense (Guss.) Strohl., a rare endemic species from the Madonie mountains (Sicily), was tested in vitro for its antimicrobial activity against bacterial reference strains and antiproliferative activity against K562 (human chronic myelogenous leukemia), HL-60 (human leukemia) and L1210 (murine leukemia) cell lines. The acetone extract showed antiproliferative IC 50 values in the range of 14–0.27 μg/ml.

Antineoplastic AgentsHL-60 CellsBiologyPlant RootsAcetoneMiceAnti-Infective Agentshemic and lymphatic diseasesDrug DiscoverymedicinePeucedanum nebrodenseCoumarins Antimicrobial Antiproliferative activityAnimalsHumansLeukemia L1210PharmacologyTraditional medicinePlant ExtractsBiological activityPlant Components AerialAntimicrobialmedicine.diseaseIn vitroLeukemiaCell cultureK562 CellsPeucedanum nebrodenseCell DivisionChronic myelogenous leukemiaK562 cellsApiaceaeJournal of ethnopharmacology
researchProduct

Farmed Gilthead Sea Bream (Sparus aurata) by-Products Valorization: Viscera Oil ω-3 Enrichment by Short-Path Distillation and In Vitro Bioactivity Ev…

2021

This study shows a pilot scale protocol aimed to obtain an omega 3-enriched oil after the processing of farmed gilthead sea bream viscera (SBV)

Antioxidant030309 nutrition & dieteticsThiobarbituric acidFish farmingmedicine.medical_treatmentShort path distillationPharmaceutical SciencePilot ProjectsnutraceuticsAntioxidantsMice03 medical and health scienceschemistry.chemical_compound0404 agricultural biotechnologySettore AGR/20 - Zoocolture3T3-L1 CellsFatty Acids Omega-3Drug DiscoveryAdipocytesTBARSmedicineAnimalsPeroxide valueFood scienceSettore BIO/06 - Anatomia Comparata E CitologiaPharmacology Toxicology and Pharmaceutics (miscellaneous)by-productslcsh:QH301-705.5DistillationWaste Productschemistry.chemical_classification0303 health sciencesAdipogenesisomega-3 fatty acidsFatty acid04 agricultural and veterinary sciences040401 food scienceSea Breamfish oilsOxidative StressViscerachemistryaquaculturelcsh:Biology (General)BassPolyunsaturated fatty acidMarine Drugs
researchProduct

Effects of Ultrasound-Assisted Extraction and Solvent on the Phenolic Profile, Bacterial Growth, and Anti-Inflammatory/Antioxidant Activities of Medi…

2020

© 2020 by the authors.

Antioxidant030309 nutrition & dieteticsmedicine.medical_treatmentAnti-Inflammatory AgentsPharmaceutical ScienceBacterial growthAntioxidantsMass SpectrometryAnti-inflammatory responseAnalytical Chemistrychemistry.chemical_compoundantioxidant methodsLiquid chromatography–mass spectrometryDrug DiscoveryFood scienceCarotenoidchemistry.chemical_classificationreactive oxygen species0303 health sciencesAntioxidant methods04 agricultural and veterinary sciences040401 food scienceSolventUltrasonic WavesChemistry (miscellaneous)Molecular MedicineTECNOLOGIA DE ALIMENTOSmedicine.drug_classArticleBioactive compoundsAnti-inflammatorylcsh:QD241-44103 medical and health sciencesExtraction techniques0404 agricultural biotechnologyPhenolslcsh:Organic chemistryextraction techniquesOleamedicinePhysical and Theoretical ChemistryEthanolbioactive compoundsPlant ExtractsOrganic ChemistryExtraction (chemistry)FicusLC-MSPlant LeaveschemistrySpectrometry Mass Matrix-Assisted Laser Desorption-Ionizationanti-inflammatory responseSolventsReactive oxygen speciesChromatography LiquidMolecules
researchProduct

Antioxidant Pathways in Alzheimers Disease: Possibilities of Intervention

2011

Alzheimer's disease (AD) is closely related to the occurrence of oxidative stress. It was claimed that all pathophysiological mechanisms involved in the onset and progression of AD are related to oxidative stress. Thus, it is important to evaluate if there is oxidative stress as well as the mechanism by which this happens in AD patients as well as in animal models of AD. Extracellular plaques of amyloid b peptides (Aβ), a hallmark of the disease, have been postulated to be more protective than damaging in terms of oxidative stress because they may be chemical sinks in which heavy metals are placed. More than a decade ago we reasoned that damage due to Ab might be caused not by extracellular…

AntioxidantAmyloidmedicine.medical_treatmentRespiratory chainNerve Tissue ProteinsBiologyPharmacologymedicine.disease_causeAntioxidantsAlzheimer DiseaseDrug DiscoverymedicineExtracellularAnimalsHumansMolecular Targeted TherapyPharmacologychemistry.chemical_classificationReactive oxygen speciesEstradiolVitamin Emedicine.diseaseUp-RegulationOxidative StressNeuroprotective AgentschemistryDietary SupplementsImmunologyAlzheimer's diseaseReactive Oxygen SpeciesOxidative stressCurrent Pharmaceutical Design
researchProduct

Dietary polyphenols in chemoprevention and synergistic effect in cancer: Clinical evidences and molecular mechanisms of action

2020

Abstract Background Epidemiological studies has revealed that a diet rich in fruits and vegetables could lower the risk of certain cancers. In this setting, natural polyphenols are potent anticancer bioactive compounds to overcome the non-target specificity, undesirable cytotoxicity and high cost of treatment cancer chemotherapy. Purpose The review focuses on diverse classifications of the chemical diversity of dietary polyphenol and their molecular targets, modes of action, as well as preclinical and clinical applications in cancer prevention. Results The dietary polyphenols exhibit chemo-preventive activity through modulation of apoptosis, autophagy, cell cycle progression, inflammation, …

AntioxidantCell cycle checkpointmedicine.medical_treatmentPharmaceutical ScienceAntineoplastic Agentsmedicine.disease_causeChemopreventionDietary PolyphenolMetastasisNeoplasmsDrug DiscoverymedicineHumansPharmacologyCancer preventionbusiness.industryPolyphenolsfood and beveragesCancermedicine.diseaseDietComplementary and alternative medicinePolyphenolCancer researchMolecular MedicinebusinessCarcinogenesisPhytomedicine
researchProduct

Tiliroside and gnaphaliin inhibit human low density lipoprotein oxidation.

2004

Two flavonoids, gnaphaliin and tiliroside, isolated from Helichrysum italicum, were studied in vitro for their capacity to inhibit Cu(2+)-induced human low density lipoprotein (LDL) and diluted plasma oxidation. LDL oxidation was monitored by conjugated diene, thiobarbituric acid-reactive substances (TBARS) formation and electrophoretic mobility on agarose gel. Gnaphaliin and tiliroside increased the lag-phase for diene conjugate production in a dose-dependent manner. The reduction of TBARS production confirmed the antioxidant activity of gnaphaliin and tiliroside with 50% inhibitory concentration (IC(50)) values of 8.0+/-3.9 microM and 7.0+/-2.6 microM respectively. Furthermore, the flavon…

AntioxidantCopper Sulfatemedicine.medical_treatmentProbucolPharmacognosyThiobarbituric Acid Reactive SubstancesLipid peroxidationchemistry.chemical_compoundInhibitory Concentration 50Drug DiscoverymedicineTBARSHumansBenzopyransIC50PharmacologyFlavonoidsHelichrysumChromatographyPlant ExtractsGeneral MedicineCholesterol LDLPlant Components AerialFlavonesLipoproteins LDLBiochemistrychemistryLow-density lipoproteinAgaroseLipid Peroxidationmedicine.drugPhytotherapyFitoterapia
researchProduct

Preparation of C-23 esterified silybin derivatives and evaluation of their lipid peroxidation inhibitory and DNA protective properties.

2009

A diverse series of C-23 esterified silybin derivatives (1a-n) were designed and synthesized. The antioxidative properties of these compounds were evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and superoxide anion radical scavenging, ferrous ion chelation, and inhibition of rat liver homogenate lipid peroxidation. Their protective effects on the prevention of hydrogen peroxide induced DNA damage were also investigated. Most of the synthesized compounds exhibited more effective antioxidant activities than silybin. The esterified silybin analogues displayed satisfactory performance especially on iron chelation and antiperoxidative activity. Compound 1n in particular exhibited remarkable a…

AntioxidantDNA damageDPPHmedicine.medical_treatmentClinical BiochemistryPharmaceutical ScienceSilibininBiochemistryLipid peroxidationchemistry.chemical_compoundStructure-Activity RelationshipDrug DiscoverymedicineAnimalsChelationMolecular BiologyChemistryOrganic ChemistryFree Radical ScavengersFree radical scavengerRatsBiochemistrySilybinMolecular MedicineLipid PeroxidationQuercetinNuclear chemistryDNA DamageSilymarinBioorganicmedicinal chemistry
researchProduct