Search results for "DOM"
showing 10 items of 12668 documents
Damping Provision by Different Virtual Synchronous Machine Schemes
2020
The adoption of virtual synchronous machine (VSM) schemes for the control of power converters is gaining more and more attention both in academia and industry. The VSM control strategies fall into the category of grid-forming converter controls, and they are intended for a range of different applications, providing specific services and different kinds of support to the grid. The paper investigates the possibility of damping provision to the system by VSM power converters. Different schemes are considered and compared, showing the opportunity of the investigated solutions through combined modal and time domain analyses. A specific modification of the power synchronization loop is recognized…
Conformational Dynamics of the Dengue Virus Protease Revealed by Fluorescence Correlation and Single-Molecule FRET Studies.
2021
The dengue virus protease (DENV-PR) represents an attractive target for counteracting DENV infections. It is generally assumed that DENV-PR can exist in an open and a closed conformation and that active site directed ligands stabilize the closed state. While crystal structures of both the open and the closed conformation were successfully resolved, information about the prevalence of these conformations in solution remains elusive. Herein, we address the question of whether there is an equilibrium between different conformations in solution which can be influenced by addition of a competitive inhibitor. To this end, DENV-PR was statistically labeled by two dye molecules constituting a FRET …
Human Papillomavirus Types 16, 18, and 31 Share Similar Endocytic Requirements for Entry
2013
ABSTRACT Human papillomavirus type 18 (HPV18), one of the HPVs with malignant potential, enters cells by an unknown endocytic mechanism. The key cellular requirements for HPV18 endocytosis were tested in comparison to those for HPV16 and -31 endocytoses. HPV18 (like HPV16 and -31) entry was independent of clathrin, caveolin, dynamin, and lipid rafts but required actin polymerization and tetraspanin CD151, and the viruses were routed to the same LAMP-1-positive compartment. Hence, the viruses shared similar cellular requirements for endocytic entry.
Impact of VP1-Specific Protein Sequence Motifs on Adeno-Associated Virus Type 2 Intracellular Trafficking and Nuclear Entry
2012
ABSTRACT Adeno-associated virus type 2 (AAV2) has gained much interest as a gene delivery vector. A hallmark of AAV2-mediated gene transfer is an intracellular conformational change of the virus capsid, leading to the exposure of infection-relevant protein domains. These protein domains, which are located on the N-terminal portion of the structural proteins VP1 and VP2, include a catalytic phospholipase A 2 domain and three clusters of basic amino acids. We have identified additional protein sequence motifs located on the VP1/2 N terminus that also proved to be obligatory for virus infectivity. These motifs include signals that are known to be involved in protein interaction, endosomal sort…
Lipid Binding Controls Dimerization of the Coat Protein p24 Transmembrane Helix
2019
Abstract Coat protein (COP) I and COP II complexes are involved in the transport of proteins between the endoplasmic reticulum and the Golgi apparatus in eukaryotic cells. The formation of COP I/II complexes at membrane surfaces is an early step in vesicle formation and is mastered by p24, a type I transmembrane protein. Oligomerization of p24 monomers was suggested to be mediated and/or stabilized via interactions within the transmembrane domain, and the p24 transmembrane helix appears to selectively bind a single sphingomyelin C18:0 molecule. Furthermore, a potential cholesterol-binding sequence has also been predicted in the p24 transmembrane domain. Thus, sphingomyelin and/or cholestero…
Clathrin- and Caveolin-Independent Entry of Human Papillomavirus Type 16—Involvement of Tetraspanin-Enriched Microdomains (TEMs)
2008
BACKGROUND: Infectious entry of human papillomaviruses into their host cells is an important step in the viral life cycle. For cell binding these viruses use proteoglycans as initial attachment sites. Subsequent transfer to a secondary receptor molecule seems to be involved in virus uptake. Depending on the papillomavirus subtype, it has been reported that entry occurs by clathrin- or caveolin-mediated mechanisms. Regarding human papillomavirus type 16 (HPV16), the primary etiologic agent for development of cervical cancer, clathrin-mediated endocytosis was described as infectious entry pathway. METHODOLOGY/PRINCIPAL FINDINGS: Using immunofluorescence and infection studies we show in contra…
A Perturbed Cauchy Viscoelastic Problem in an Exterior Domain
2023
A Cauchy viscoelastic problem perturbed by an inverse-square potential, and posed in an exterior domain of RN, is considered under a Dirichlet boundary condition. Using nonlinear capacity estimates specifically adapted to the non-local nature of the problem, the potential function and the boundary condition, we establish sufficient conditions for the nonexistence of weak solutions.
4-5 gadu vecu bērnu radošās darbības veicināšana vizuālās mākslas nodarbībās
2018
Kvalifikācijas tēmas nosaukums ir “4-5 gadu vecu bērnu radošās darbības veicināšana vizuālās mākslas nodarbībās”, darbs sastāv no ievada, 3 nodaļām, 8 apakšnodaļām, kas apkopotas 55 lpp. Darbā ievietoti 13 attēli, 8 tabulas, 7 pielikumi , 56 literatūras avoti. Darba autore: Revita Vildava Pētījuma mērķis: Pētīt skolotāja iespējas sekmēt bērnu radošo darbību vizuālās mākslas nodarbībās. Pētījuma objekts: Vizuālās mākslas nodarbības pirmsskolā Pētījuma priekšmets: 4-5 gadu vecu bērnu radošā darbība Pētījuma hipotēze: Vizuālās mākslas nodarbībās ir iespējams sekmēt bērna radošo darbību, izmantojot dažādas mākslas veidus, tehnikas, palīglīdzekļus. Pētījuma teorētiskajā daļā tiek aprakstīts, kas…
Skolēnu radošās domāšanas veicināšana vizuālajā mākslā sākumskolas 4.klasē
2022
Bakalaura darbā “Skolēnu radošās domāšanas veicināšana vizuālajā mākslā sākumskolas 4. klasē” ietvaros tiek teorētiski un praktiski izpētīts kā radošums palīdz mācību procesā, un kā šīs prasmes attīstību var mobilizēt caur vizuālās mākslas priekšmetu ar dažādiem uzdevumiem, kas tiek veikti mācību stundās. Darba mērķis ir izpētīt radošumu, tā attīstību skolēnos un skolotāja ietekmi uz visu mācīšanas un mācīšanās procesu. Pētījumā tika izvirzīta hipotēze: skolēnu radošā domāšana vizuālajā mākslā sākumskolas 4.klasē tiks veicināta, ja skolotājs: 1)Uzdodot stundās veikt dizaina uzdevumus, veicinās skolēniem radošo domāšanu. 2)Mācību saturu sasaistīs ar ikdienu un veidos uzdevumus, kas pielīdzin…
2-3 gadīgu bērnu vārdu krājuma aktivizēšana rotaļās
2019
Bakalaura darba autors: Marija Smirnova Bakalaura darba temats: “2–3 gadīgu bērnu vārdu krājuma aktivizēšana rotaļās” Mērķis: Teorētiski un praktiski izzināt dažādu rotaļu izmantošanas iespējas, kas veicinātu 2-3 gadīgu bērnu runas attīstību. Darba pirmajā nodaļā tiek pētīta un analizēta pedagoģijas un psiholoģijas zinātniskā literatūra par valodas apgūšanas faktoriem un runas attīstību. Tiek pētīts rotaļas raksturojums un skaidrojums. Dažādu autoru atziņās tiek noskaidrotas bērnu valodas attīstības īpatnības. Uzmanība tiek vērsta 2 – 3 gadīgu bērnu runas raksturojumam. Otrajā nodaļā tika veikts empīriskais pētījums, kā ar rotaļu palīdzību var aktivizēt vārdu krājumu jaunākajā pirmsskolas v…