Search results for "DOMAIN"
showing 10 items of 2485 documents
Mutations in DNA Binding and Transactivation Domains Affect the Dynamics of Parvovirus NS1 Protein
2013
ABSTRACT The multifunctional replication protein of autonomous parvoviruses, NS1, is vital for viral genome replication and for the control of viral protein production. Two DNA-interacting domains of NS1, the N-terminal and helicase domains, are necessary for these functions. In addition, the N and C termini of NS1 are required for activation of viral promoter P38. By comparison with the structural and biochemical data from other parvoviruses, we identified potential DNA-interacting amino acid residues from canine parvovirus NS1. The role of the identified amino acids in NS1 binding dynamics was studied by mutagenesis, fluorescence recovery after photobleaching, and computer simulations. Mu…
Consensus guidelines for the detection of immunogenic cell death
2014
Apoptotic cells have long been considered as intrinsically tolerogenic or unable to elicit immune responses specific for dead cell-associated antigens. However, multiple stimuli can trigger a functionally peculiar type of apoptotic demise that does not go unnoticed by the adaptive arm of the immune system, which we named "immunogenic cell death" (ICD). ICD is preceded or accompanied by the emission of a series of immunostimulatory damage-associated molecular patterns (DAMPs) in a precise spatiotemporal configuration. Several anticancer agents that have been successfully employed in the clinic for decades, including various chemotherapeutics and radiotherapy, can elicit ICD. Moreover, defect…
Hajłasz–Sobolev imbedding and extension
2011
Abstract The author establishes some geometric criteria for a Hajlasz–Sobolev M ˙ ball s , p -extension (resp. M ˙ ball s , p -imbedding) domain of R n with n ⩾ 2 , s ∈ ( 0 , 1 ] and p ∈ [ n / s , ∞ ] (resp. p ∈ ( n / s , ∞ ] ). In particular, the author proves that a bounded finitely connected planar domain Ω is a weak α -cigar domain with α ∈ ( 0 , 1 ) if and only if F ˙ p , ∞ s ( R 2 ) | Ω = M ˙ ball s , p ( Ω ) for some/all s ∈ [ α , 1 ) and p = ( 2 − α ) / ( s − α ) , where F ˙ p , ∞ s ( R 2 ) | Ω denotes the restriction of the Triebel–Lizorkin space F ˙ p , ∞ s ( R 2 ) on Ω .
Binge administration of 3,4-methylenedioxymethamphetamine ("ecstasy") impairs the survival of neural precursors in adult rat dentate gyrus.
2006
3,4-Methylenedioxymethamphetamine (MDMA) is a potent stimulant and hallucinogenic drug whose ability to regulate neurogenesis in the adult has not been previously investigated. We used 5'-bromo-2-deoxyuridine (BrdU) and Ki-67 as mitotic markers, and doublecortin (DCX) as a marker of immature neurons, to study proliferation, survival and maturation of adult-generated cells in the dentate gyrus (DG) of the hippocampus following binge administration of MDMA (8 injections of 5 mg/kg at 6 h intervals). The results showed that MDMA treatment did not affect cytogenesis in the DG, but significantly decreased the survival rate of cells incorporated after 2 weeks to the granular layer of the DG by ca…
The boundary Harnack inequality for infinity harmonic functions in Lipschitz domains satisfying the interior ball condition
2008
Abstract In this note, we give a short proof for the boundary Harnack inequality for infinity harmonic functions in a Lipschitz domain satisfying the interior ball condition. Our argument relies on the use of quasiminima and the notion of comparison with cones.
Crisis and EU Foreign and Security Policy: An Introduction
2020
This chapter sums up the main findings in the four papers discussing the impact of various external crises on the development of the EU’s Common Foreign and Security Policy, the CFSP. Chapters discuss the EU’s foreign and security policy responses to the Russian annexation of Crimea and the ongoing war in Ukraine, the EU’s crisis management capacities, and the impact of crises on EU–US relations. Despite member states’ traditional reluctance to integrate further in this domain of EU policy, the CFSP is not breaking down. Instead, all the chapters suggest that the EU is able to cope with crises and that overall, crises and increased uncertainty have led to a strengthening of the CFSP mainly …
Transferable and Negotiated Knowledge
2002
• Summary: This article explores the knowledge of community-based social workers in the context of an action research project aimed at exploring the practitioners’ own descriptions of their knowledge and expertise on the theme of spatial marginalization. • Findings: The knowledge of social workers seemed to be based on service users’ experiences and case examples, on value and moral constructions, and it was created from experience, by doing and in action. It was local and contextual, in some sense silent but shared through a discussion process. It was not based on empirically based scientific research understood in the traditional sense; rather, social workers resorted to practical knowle…
Structural Domains of the Bacillus thuringiensis Vip3Af Protein Unraveled by Tryptic Digestion of Alanine Mutants
2019
Vip3 proteins are increasingly used in insect control in transgenic crops. To shed light on the structure of these proteins, we used the approach of the trypsin fragmentation of mutants altering the conformation of the Vip3Af protein. From an alanine scanning of Vip3Af, we selected mutants with an altered proteolytic pattern. Based on protease digestion patterns, their effect on oligomer formation, and theoretical cleavage sites, we generated a map of the Vip3Af protein with five domains which match some of the domains proposed independently by two in silico models. Domain I ranges amino acids (aa) 12&ndash
Monotonicity and local uniqueness for the Helmholtz equation
2017
This work extends monotonicity-based methods in inverse problems to the case of the Helmholtz (or stationary Schr\"odinger) equation $(\Delta + k^2 q) u = 0$ in a bounded domain for fixed non-resonance frequency $k>0$ and real-valued scattering coefficient function $q$. We show a monotonicity relation between the scattering coefficient $q$ and the local Neumann-Dirichlet operator that holds up to finitely many eigenvalues. Combining this with the method of localized potentials, or Runge approximation, adapted to the case where finitely many constraints are present, we derive a constructive monotonicity-based characterization of scatterers from partial boundary data. We also obtain the local…
An optimal Poincaré-Wirtinger inequality in Gauss space
2013
International audience; Let $\Omega$ be a smooth, convex, unbounded domain of $\mathbb{R}^N$. Denote by $\mu_1(\Omega)$ the first nontrivial Neumann eigenvalue of the Hermite operator in $\Omega$; we prove that $\mu_1(\Omega) \ge 1$. The result is sharp since equality sign is achieved when $\Omega$ is a $N$-dimensional strip. Our estimate can be equivalently viewed as an optimal Poincaré-Wirtinger inequality for functions belonging to the weighted Sobolev space $H^1(\Omega,d\gamma_N)$, where $\gamma_N$ is the $N$% -dimensional Gaussian measure.