Search results for "DOMAIN"
showing 10 items of 2485 documents
MORT1/FADD is involved in liver regeneration
2006
AIM: To explore the role of the adaptor molecule in liver regeneration after partial hepatectomy (PH). METHODS: We used transgenic mice expressing an N-terminal truncated form of MORT1/FADD under the control of the albumin promoter. As previously shown, this transgenic protein abrogated CD95- and CD120a-mediated apoptosis in the liver. Cyclin A expression was detected using Western blotting. ELISA and RT-PCR were used to detect IL-6 and IL-6 mRNA, respectively. DNA synthesis in liver tissue was measured by BrdU staining. RESULTS: Resection of 70% of the liver was followed by a reduced early regenerative response in the transgenic group at 36 h. Accordingly, 36 h after hepatectomy, cyclin A …
Transcriptional regulation of nucleoredoxin-like genes takes place on a daily basis in the retina and pineal gland of rats.
2015
AbstractThe nucleoredoxin-like gene Nxnl1 (Txnl6) and its paralogue Nxnl2 encode the rod-derived cone viability factors (RdCVF and RdCVF2), which increase the resistance to photooxidative damage and have therapeutic potential for the survival of cones in retinitis pigmentosa. In this study, the transcription of Nxnl genes was investigated as a function of the day/night cycle in rats. The transcript levels of Nxnl1 and Nxnl2 were seen to display daily rhythms with steadily increasing values during the light phase and peak expression around dark onset in preparations of whole retina, photoreceptor cells and—but only in regard to Nxnl1—in photoreceptor-related pinealocytes. The cycling of Nxnl…
Acoustics of snoring and automatic snore sound detection in children
2017
Objective Acoustic analyses of snoring sounds have been used to objectively assess snoring and applied in various clinical problems for adult patients. Such studies require highly automatized tools to analyze the sound recordings of the whole night's sleep, in order to extract clinically relevant snore- related statistics. The existing techniques and software used for adults are not efficiently applicable to snoring sounds in children, basically because of different acoustic signal properties. In this paper, we present a broad range of acoustic characteristics of snoring sounds in children (N = 38) in comparison to adult (N = 30) patients. Approach Acoustic characteristics of the signals we…
Adaptive immune responses to SARS-CoV-2 in recovered severe COVID-19 patients
2021
ABSTRACTObjectivesThere is an imperative need to determine the durability of adaptive immunity to SARS-CoV-2. We enumerated SARS-CoV-2-reactive CD4+ and CD8+ T cells targeting S1 and M proteins and measured RBD-specific serum IgG over a period of 2-6 months after symptoms onset in a cohort of subjects who had recovered from severe clinical forms of COVID-19.MethodsWe recruited 58 patients (38 males and 20 females; median age, 62.5 years), who had been hospitalized with bilateral pneumonia, 60% with one or more comorbidities. IgG antibodies binding to SARS-CoV-2 RBD were measured by ELISA. SARS-CoV-2-reactive CD69+-expressing-IFNγ-producing-CD4+ and CD8+ T cells were enumerated in heparinize…
Human Oxidation-Specific Antibodies Reduce Foam Cell Formation and Atherosclerosis Progression
2011
ObjectivesWe sought to assess the in vivo importance of scavenger receptor (SR)–mediated uptake of oxidized low-density lipoprotein (OxLDL) in atherogenesis and to test the efficacy of human antibody IK17-Fab or IK17 single-chain Fv fragment (IK17-scFv), which lacks immunologic properties of intact antibodies other than the ability to inhibit uptake of OxLDL by macrophages, to inhibit atherosclerosis.BackgroundThe unregulated uptake of OxLDL by macrophage SR contributes to foam cell formation, but the importance of this pathway in vivo is uncertain.MethodsCholesterol-fed low-density lipoprotein receptor knockout (LDLR−/−) mice were treated with intraperitoneal infusion of human IK17-Fab (2.…
Monocytes from patients with rheumatoid arthritis and type 2 diabetes mellitus display an increased production of interleukin (IL)-1β via the nucleot…
2015
Summary A better understanding about the mechanisms involved in the pathogenesis of type 2 diabetes mellitus (T2D) showed that inflammatory cytokines such as tumour necrosis factor (TNF) and interleukin (IL)-1β play a pivotal role, mirroring data largely reported in rheumatoid arthritis (RA). IL-1β is produced mainly by monocytes (MO), and hyperglycaemia may be able to modulate, in the cytoplasm of these cells, the assembly of a nucleotide-binding domain and leucine-rich repeat containing family pyrin (NLRP3)-inflammosome, a cytosolic multi-protein platform where the inactive pro-IL-1β is cleaved into active form, via caspase-1 activity. In this paper, we evaluated the production of IL-1 β …
Developing a blog trust scale via an international Delphi panel
2020
Abstract Although trust is one of the most widespread and studied constructs in marketing and management, its definition, versatility and different application areas may have fostered a certain dispersion when measuring this concept. Despite the increasing importance of blogs, specific scales for, and measures of, trust have not been considered in the literature. This paper proposes a scale to measure online trust in the blog domain (Blog Trust Scale, BTS) through the development of an international Delphi panel consisting of 14 experts from 5 different countries. As a result, we have developed a solid measurement instrument of online trust in blogs, composed of 12 items and tested in four …
Where to acquire knowledge: Adapting knowledge management to financial institutions
2016
Abstract This research seeks to determine which sources of knowledge have the greatest effect on financial entities' knowledge acquisition and management. A review of the literature on knowledge management examines four key knowledge sources: Human resources, organizational management, technology adoption, and the business environment. The study performs pairwise comparisons of variables through the analytic hierarchy process (AHP), using a scale that captures the importance of each criterion, thereby simplifying the decision process. Results show that human resources and new technology adoption are the most effective sources of knowledge acquisition and management. Specifically, one of the…
Designing a multi-layer edge-computing platform for energy-efficient and delay-aware offloading in vehicular networks
2021
Abstract Vehicular networks are expected to support many time-critical services requiring huge amounts of computation resources with very low delay. However, such requirements may not be fully met by vehicle on-board devices due to their limited processing and storage capabilities. The solution provided by 5G is the application of the Multi-Access Edge Computing (MEC) paradigm, which represents a low-latency alternative to remote clouds. Accordingly, we envision a multi-layer job-offloading scheme based on three levels, i.e., the Vehicular Domain, the MEC Domain and Backhaul Network Domain. In such a view, jobs can be offloaded from the Vehicular Domain to the MEC Domain, and even further o…
Mass transport problems obtained as limits of p-Laplacian type problems with spatial dependence
2014
Abstract. We consider the following problem: given a bounded convex domain Ω ⊂ ℝ N ${\Omega \subset \mathbb {R}^N}$ we consider the limit as p → ∞ of solutions to - div ( b p - p | D u | p - 2 D u ) = f + - f - ${- \operatorname{div} (b_{p}^{-p} |Du|^{p-2} Du)=f_+ - f_-}$ in Ω and b p - p | D u | p - 2 ∂ u ∂ η = 0 ${ b_{p}^{-p} |Du|^{p-2} \frac{\partial u}{\partial \eta }=0}$ on ∂ Ω ${\partial \Omega }$ . Under appropriate assumptions on the coefficients bp that in particular verify that lim p → ∞ b p = b ${ \lim _{p\rightarrow \infty } b_p = b }$ uniformly in Ω ¯ ${\overline{\Omega }}$ , we prove that there is a uniform limit of u p j ${u_{p_j}}$ (along a sequence p j → ∞ ${p_j \rightarrow…