Search results for "DOMAIN"

Interpretación social y accesibilidad. Una propuesta de conexión disciplinar

2020

El objetivo de este estudio es ofrecer argumentos para entender la actividad propia de la interpretación social como parte del dominio genérico de la accesibilidad. Al hacerlo no ampliamos en realidad el concepto de accesibilidad, sino que reforzamos aquello que justifica el uso de este término, para diferenciar una parcela de actividad dentro de la práctica general de la traducción y la interpretación. Con el tema de la accesibilidad sucede que se ha manejado una etiqueta sin una delimitación conceptual previa de lo que se pretende designar por medio de ella. En este estudio nos ocupamos brevemente de esta tarea de aproximación conceptual, con el objetivo de incluir bajo el abanico de las …

Characterization of denitrification gene clusters of soil bacteria via a metagenomic approach

2009

International audience; Denitrification is a microbial respiratory process contributing to the emission of greenhouse gas. The study of denitrifying bacteria, like that of others, is hindered by characteristics that can prevent up to 99% of soil bacteria from being cultivated in vitro. New approaches based on the direct extraction of DNA from the natural environment and PCR amplifications can overcome limitations due to bacterial unculturability, but until now their application to denitrification genes has led only to the recovery of partial sequences for some of these genes.Our goals in this study were to apply a metagenomic approach characterized by cloning of DNA extracted from soil and …

Ontological supports of knowledge: knowledge creation and analytical knowledge

2011

PurposeThe purpose of this paper is to examine, from the perspective of different theoretical approaches, the relationship that exists between different ontological supports of knowledge and knowledge itself (the way it is created and its characteristics).Design/methodology/approachThe paper proposes two different types of knowledge (knowledge of concrete situations and abstract knowledge) and two approaches (the constructivist view and the cognitive view) and provides a general classification of the different knowledge types. Second, it examines the underlying ontological support‐knowledge creation, characteristics or types of knowledge relationship in different approaches. Finally, conclu…

Detergent Properties Influence the Stability of the Glycophorin A Transmembrane Helix Dimer in Lysophosphatidylcholine Micelles

2012

AbstractDetergents might affect membrane protein structures by promoting intramolecular interactions that are different from those found in native membrane bilayers, and fine-tuning detergent properties can be crucial for obtaining structural information of intact and functional transmembrane proteins. To systematically investigate the influence of the detergent concentration and acyl-chain length on the stability of a transmembrane protein structure, the stability of the human glycophorin A transmembrane helix dimer has been analyzed in lyso-phosphatidylcholine micelles of different acyl-chain length. While our results indicate that the transmembrane protein is destabilized in detergents w…

Models Versus Model Descriptions

2021

In the development of computer-based systems, modelling is often advocated in addition to programming, in that it helps in reflecting the application domain and that it makes the design and experiment activities of development more efficient. However, there is disagreement about what models are and how they can be used in software systems development. In this paper, we present the Scandinavian approach to modelling, which makes a clear distinction between models and model descriptions. This paper explains the connections between models, descriptions, systems, and executions. Combining the Scandinavian approach with the Kiel notion of model, we establish that both descriptions and executions…

2020

Monitoring of the action of drugs in melanoma cells by dynamic laser speckle

2014

Abstract. This work presents the development of a protocol based on the dynamic laser speckle designed tomonitor the reaction of cancer cells of line MEL-RC08 to the application of the drug Colcemid in two differentconcentrations: 0.2 and 0.4 μg∕mL. The protocol was designed using the forward scattering approach with anHe-Ne laser of 632.8 nm illuminating the samples, a control, and two variations of Colcemid, being monitoredalong 8 h. The data were analyzed numerically in the time and in the frequency domain, and the results pre-sented the ability of the technique to monitor the action of the drug, particularly Colcemid (0.4 μg∕mL). © 2014Society of Photo-Optical Instrumentation Engineers …

Indispensability and Effectiveness of Diagrams in Molecular Biology

2019

Abstract: In this paper I aim to defend a twofold thesis. On one hand, I will support, against Perini (2005), the indispensability of diagrams when structurally complex biomolecules are concerned, since it is not possible to satisfactorily use linguistic-sentential representations at that domain. On the other hand, even when diagrams are dispensable I will defend than they will generally be more effective than other representations in encoding biomolecular knowledge, relying on Kulvicki-Shimojima’s diagrammatic effectiveness thesis. Finally, I will ground many epistemic virtues of biomolecular diagrams (understandability, explanatory power, prediction and hypothesis evaluation) on their cog…

The sensor kinase DcuS of Escherichia coli: two stimulus input sites and a merged signal pathway in the DctA/DcuS sensor unit

2012

Abstract The membrane-integral sensor kinase DcuS of Escherichia coli consists of a periplasmically located sensory PASP domain, transmembrane helices TM1 and TM2, a cytoplasmic PASC domain and the kinase domain. Stimulus (C4-dicarboxylate) binding at PASP is required to stimulate phosphorylation of the kinase domain, resulting in phosphoryl transfer to the response regulator DcuR. PASC functions as a signaling device or a relay in signal transfer from TM2 to the kinase. Phosphorylated DcuR induces the expression of the target genes. Sensing by DcuS requires the presence of the C4-dicarboxylate transporter DctA during aerobic growth. DctA forms a sensor unit with DcuS, and a short C-termina…

Gaussian and non-Gaussian stochastic sensitivity analysis of discrete structural systems

2000

Abstract The derivatives of the response of a structural system with respect to the system parameters are termed sensitivities. They play an important role in assessing the effect of uncertainties in the mathematical model of the system and in predicting changes of the response due to changes of the design parameters. In this paper, a time domain approach for evaluating the sensitivity of discrete structural systems to deterministic, as well as to Gaussian or non-Gaussian stochastic input is presented. In particular, in the latter case, the stochastic input has been assumed to be a delta-correlated process and, by using Kronecker algebra extensively, cumulant sensitivities of order higher t…