Search results for "DOT"

Role of the protein C receptor in cancer progression

2014

The hemostatic system plays pleiotropic roles in cancer progression by shaping the tumor microenvironment and metastatic niches through thrombin-dependent fibrin deposition and platelet activation. Expanding experimental evidence implicates coagulation protease receptors expressed by tumor cells as additional players that directly influence tumor biology. Pro-angiogenic G protein-coupled signaling of TF through protease activated receptor 2 and regulation of tumor cell and vascular integrins through ligation by alternative spliced TF are established pathways driving tumor progression. Our recent work shows that the endothelial protein C receptor (EPCR), a stem cell marker in hematopoietic, …

From Regenerative Medicine to Endothelial Progenitor Cells as Potential Candidates

2017

A promising branch of translational medicine is Regenerative Medicine (RegMed). RegMed aims to improve health and quality of life by restoring, maintaining or enhancing tissues and functions of organs. In order to achieve this, RegMed utilizes several approaches. Among these, cell-based therapy represents the central focus of RegMed. Several cellular types have been considered for the investigation and application of regenerative cell therapy in disease organs. and bone marrow (BM)-derived endothelial progenitor cells (BM EPCs) are emerging as potential candidates. Here, particular emphasis is given to the description of their biological features, for understanding their relevance and poten…

In Vitro Differentiation of Mouse Embryonic Stem Cells: Hematopoietic and Vascular Cell Types

2003

Publisher Summary Embryonic stem cells have been posited as sources of differentiated cell types for regenerative medicine. One of the most enticing cell types is the recently described endothelial progenitor cell (EPC) which can contribute to blood vessels and is a candidate for therapy against vascular diseases. This chapter describes an in vitro differentiation system, which results in the generation of endothelial, smooth muscle, and hematopoietic cells from ES cells. An ES-derived equivalent of the lateral plate mesoderm (LPM) has been generated, a critical source of vascular and hematopoietic cells during embryonic development. This in vitro differentiation system is useful for the an…

Protease inhibitor atazanavir induces leukocyte-endothelial cell interactions in the microvasculature

2008

Impact of Ceramic and Metallic Nano-scaled Particles on Endothelial Cell Functionsin vitro

2007

The sections in this article are Introduction Origin of Particles in the Human Environment Evidence for Size-dependent Toxicity of Particles Dissemination and Interferences of Nanoparticles within the Body Endothelial Cells and Nanoparticle Exposure Testing of Nanoparticle-induced Effects on Human Endothelial Cells In Vitro Materials and Methods Cell Culture Particles Transmission Electron Microscopy (TEM) Cytotoxicity Assay Detection of Ki67 Expression Quantification of IL-8 Release in Cell Culture Supernatant Quantification of E-selectin Cell Surface Protein Expression Fluorescence Staining Statistical Analysis Results Discussion Particle Internalization Particle Cytotoxicity Pro-inflamma…

Angiotensin-(1-7) attenuates endothelial cell senescence via klotho and Nrf2 activation

2018

Outgrowth Endothelial Cells: Sources, Characteristics and Potential Applications in Tissue Engineering and Regenerative Medicine

2010

Endothelial progenitor cells from peripheral blood or cord blood are attracting increasing interest as a potential cell source for cellular therapies aiming to enhance the neovascularization of tissue engineered constructs or ischemic tissues. The present review focus on a specific population contained in endothelial progenitor cell cultures designated as outgrowth endothelial cells (OEC) or endothelial colony forming cells from peripheral blood or cord blood. Special attention will be paid to what is currently known in terms of the origin and the cell biological or functional characteristics of OEC. Furthermore, we will discuss current concepts, how OEC might be integrated in complex tissu…

Effect of phosphonate-functionalised nanoparticles on human mesenchymal stem cells and osteoclasts

2011

Inflammatory Murine Skin Responses to UV-B Light Are Partially Dependent on Endothelin-1 and Mast Cells

2006

Endothelin (ET-1) has been shown to crucially contribute to UV-induced skin responses such as tanning. To test whether ET-1 is also involved in early cutaneous reactions to UV, we assessed ET-1 skin levels in UV-irradiated mice. In correlation with the levels of UV-induced skin inflammation, ET-1 concentrations increased substantially and continually. Moreover, blocking of ET-1 receptors (ET A ) resulted in significantly decreased cutaneous inflammation following UV irradiation. When we assessed skin responses to ET-1 injections, we observed prominent mast cell degranulation and mast cell-dependent inflammation. Since mast cells also critically contributed to UV-induced inflammation, we det…

Experimental Study of the Effects of EIPA, Losartan, and BQ-123 on Electrophysiological Changes Induced by Myocardial Stretch

2015

[ES] Introducción y objetivos Se han implicado diversos mecanismos en la respuesta mecánica al estiramiento miocárdico, que incluyen la activación del intercambiador Na+/H+ por acciones autocrinas y paracrinas. Se estudia la participación de estos mecanismos en las respuestas electrofisiológicas al estiramiento agudo miocárdico mediante el análisis de los cambios inducidos con fármacos. Métodos Se analizan las modificaciones de la fibrilación ventricular inducidas por el estiramiento agudo miocárdico en corazones de conejo aislados y perfundidos utilizando electrodos múltiples epicárdicos y técnicas cartográficas. Se estudian 4 series: control (n = 9); durante la perfusión del antagonista d…