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showing 10 items of 2669 documents
Cloning and expression of a cDNA copy of the viral K28 killer toxin gene in yeast
1995
The killer toxin K28, secreted by certain killer strains of the yeast Saccharomyces cerevisiae is genetically encoded by a 1.9 kb double-stranded RNA, M-dsRNA (M28), that is present within the cell as a cytoplasmically inherited virus-like particle (VLP). For stable maintenance and replication, M28-VLPs depend on a second dsRNA virus (LA), which has been shown to encode the major capsid protein (cap) and a capsid-polymerase fusion protein (cap-pol) that provides the toxin-coding M-satellites with their transcription and replicase functions. K28 toxin-coding M28-VLPs were isolated, purified and used in vitro for the synthesis of the single-stranded M28 transcript, which was shown to be of pl…
Measuring frequency domain granger causality for multiple blocks of interacting time series
2011
In the past years, several frequency-domain causality measures based on vector autoregressive time series modeling have been suggested to assess directional connectivity in neural systems. The most followed approaches are based on representing the considered set of multiple time series as a realization of two or three vector-valued processes, yielding the so-called Geweke linear feedback measures, or as a realization of multiple scalar-valued processes, yielding popular measures like the directed coherence (DC) and the partial DC (PDC). In the present study, these two approaches are unified and generalized by proposing novel frequency-domain causality measures which extend the existing meas…
Effectiveness of Posidonia oceanica biotic indices for assessing the ecological status of coastal waters in the Saronikos Gulf (Aegean Sea, Eastern M…
2017
Biotic indices are considered key assessment tools in most national and European policies aimed at improving the quality of coastal waters. At present, several Water Framework Directive (WFD)-compliant biotic indices based on the marine angiosperm Posidonia oceanica have been developed and applied in the Mediterranean Sea. In this study, we investigated the effectiveness of four different P. oceanica indices (POMI, PREI, Valencian CS, and BiPo) in evaluating the ecological status of coastal waters in a case study area of Greece. The evaluation, comparison, and validation of the Ecological Status Class (ESC) assessments obtained by each index were based on a set of eight common sites that en…
"Ab initio" structure solution from electron diffraction data obtained by a combination of automated diffraction tomography and precession technique
2009
Using a combination of our recently developed automated diffraction tomography (ADT) module with precession electron technique (PED), quasi-kinematical 3D diffraction data sets of an inorganic salt (BaSO(4)) were collected. The lattice cell parameters and their orientation within the data sets were found automatically. The extracted intensities were used for "ab initio" structure analysis by direct methods. The data set covered almost the complete set of possible symmetrically equivalent reflections for an orthorhombic structure. The structure solution in one step delivered all heavy (Ba, S) as well as light atoms (O). Results of the structure solution using direct methods, charge flipping …
In light of the ends. Copyright hysteresis and private copy exception after the British Academy of Songwriters, Composers and Authors (BASCA) and oth…
2015
In British Academy of Songwriters, Composers and Authors (BASCA) and others v Secretary of State for Business, Innovation and Skills, the High Court of Justice in matter of private copy exception provides the twofold prime opportunity to shed light on the state of the art of copyright in the UK and to flesh out the idea of 'legal hysteresis’. I support the reintroduction of the private copy exception, possibly in a less narrow fashion, and I explain the reasons why I am confident that my expectations will be fulfilled.
A Framework of the Impact of Cyberspace on Contemporary Organizations
2006
This paper is an attempt to create a holistic approach to the problem of the impact of the elements operating in cyberspace on an organization. Firstly, a classification of the entities operating in cyberspace is offered. Next, a taxonomy of the possible types of influences of the elements of cyberspace on an organization is presented. In the following part of the paper two matrices concerning types of direct and indirect impact of the above mentioned entities are provided along with examples. Finally, conclusions are offered.
Combining EXAFS and XRay Powder Diffraction to Solve Structures Containing Heavy Atoms
2005
Determination of structures using x-ray powder diffraction is complicated if the reflection intensities are mainly influenced by the scattering from heavy atoms and the atomic coordinates of light atoms remain uncertain. A method like EXAFS, which is sensitive to short range order, gives reliable atomic distances in the surroundings of heavy atoms with a precision of ±0.02 A. The probability for obtaining the complete structure from x-ray powder diffraction increases if one includes parameters derived from EXAFS measurements as restraints during the procedure of structure solving. We demonstrate the potential of combining EXAFS and x-ray powder diffraction by solving the structure UO2[H2AsO…
Genomic investigation of a legionellosis outbreak in a persistently colonized hotel
2016
Objectives: A long-lasting legionellosis outbreak was reported between November 2011 and July 2012 in a hotel in Calpe (Spain) affecting 44 patients including six deaths. Intensive epidemiological and microbiological investigations were performed in order to detect the reservoirs. Methods: Clinical and environmental samples were tested for the presence and genetic characterization of Legionella pneumophila. Six of the isolates were subjected to whole-genome sequencing. Results: Sequencing of 14 clinical and 260 environmental samples revealed sequence type (ST) 23 as the main responsible strain for the infections. This ST was found in the spa pool, from where it spread to other hotel public …
Effect of Acoustic Wave Reflections on Space Charge Measurements with PEA Method
2018
The Pulsed Electro-Acoustic (PEA) method is the most used technique for space charge detection in solid dielectrics. The methodology is largely employed in the field of High Voltage Direct Current (HVDC) transmission and is based on the detection of acoustic waves generated by charges vibration. One of the most common problems arising during the detection is the presence of multiple reflections taking place due to the presence of means discontinuity in the different PEA cell components. This reflection phenomenon, if not well taken into account, could cause incorrect interpretation of the PEA output signal. It is easy to understand that a simulation model is a basic tool to understand the w…
Peptide Processing Is Critical for T-Cell Memory Inflation and May Be Optimized to Improve Immune Protection by CMV-Based Vaccine Vectors.
2016
Cytomegalovirus (CMV) elicits long-term T-cell immunity of unparalleled strength, which has allowed the development of highly protective CMV-based vaccine vectors. Counterintuitively, experimental vaccines encoding a single MHC-I restricted epitope offered better immune protection than those expressing entire proteins, including the same epitope. To clarify this conundrum, we generated recombinant murine CMVs (MCMVs) encoding well-characterized MHC-I epitopes at different positions within viral genes and observed strong immune responses and protection against viruses and tumor growth when the epitopes were expressed at the protein C-terminus. We used the M45-encoded conventional epitope HGI…