Search results for "Drosophila."

showing 10 items of 769 documents

The ISWI chromatin remodeler organizes the hsrω ncRNA-containing omega speckle nuclear compartments.

2011

The complexity in composition and function of the eukaryotic nucleus is achieved through its organization in specialized nuclear compartments. The Drosophila chromatin remodeling ATPase ISWI plays evolutionarily conserved roles in chromatin organization. Interestingly, ISWI genetically interacts with the hsrω gene, encoding multiple non-coding RNAs (ncRNA) essential, among other functions, for the assembly and organization of the omega speckles. The nucleoplasmic omega speckles play important functions in RNA metabolism, in normal and stressed cells, by regulating availability of hnRNPs and some other RNA processing proteins. Chromatin remodelers, as well as nuclear speckles and their assoc…

MaleCancer ResearchRNA Untranslatedlcsh:QH426-470Gene ExpressionFluorescent Antibody TechniqueRNA-binding proteinBiologyEyeHeterogeneous ribonucleoprotein particleChromosomesHeterogeneous-Nuclear RibonucleoproteinsChromatin remodelingMolecular GeneticsGeneticsmedicineAnimalsDrosophila ProteinsOmega speckleBiologyMolecular BiologyTranscription factorAllelesGenetics (clinical)Ecology Evolution Behavior and SystematicsAdenosine TriphosphatasesCell NucleusGeneticsRNA-Binding ProteinsEpistasis GeneticChromatin Assembly and DisassemblyNon-coding RNAChromatinCell biologyCell nucleuslcsh:GeneticsPhenotypemedicine.anatomical_structureTandem Repeat SequencesChromatin remodeling non coding RNALarvaEpigeneticsDrosophilaRNA InterferenceResearch ArticleTranscription FactorsPLoS Genetics
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Reduced expression of Hugl-1, the human homologue of Drosophila tumour suppressor gene lgl, contributes to progression of colorectal cancer.

2005

The human gene, human giant larvae (Hugl-1/Llg1/Lgl1) has significant homology to the Drosophila tumour suppressor gene lethal(2)giant larvae (lgl). The lgl gene codes for a cortical cytoskeleton protein, Lgl, that binds Myosin II and is involved in maintaining cell polarity and epithelial integrity. The human protein, Hugl-1 contains several conserved functional domains found in Lgl, suggesting that these proteins may have closely related functions. Whether loss of Hugl expression plays a role in human tumorigenesis has so far not been extensively investigated. Thus, we evaluated tumour tissues from 94 patients undergoing surgery for colorectal cancer (CRC) for loss of Hugl-1 transcription…

MaleCancer ResearchTranscription Geneticmedicine.disease_causeCell MovementNeoplasmsGene expressionDrosophila ProteinsIntestinal MucosaCytoskeletonReverse Transcriptase Polymerase Chain ReactionCell CycleCell migrationCell DifferentiationMiddle AgedImmunohistochemistryGene Expression Regulation NeoplasticDrosophila melanogasterDisease ProgressionFemaleColorectal NeoplasmsAdenomaAdultTumor suppressor geneBlotting WesternGreen Fluorescent ProteinsDown-RegulationBiologyCell LineDownregulation and upregulationCell Line TumorGeneticsmedicineCell AdhesionAnimalsHumansCell adhesionMolecular BiologyGeneTumor Suppressor ProteinsCarcinomaProteinsProtein Structure TertiaryCytoskeletal ProteinsMicroscopy FluorescenceTumor progressionImmunologyCancer researchCaco-2 CellsCarcinogenesisOncogene
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Two distinct genomic regions, harbouring the period and fruitless genes, affect male courtship song in Drosophila montana

2012

Acoustic signals often have a significant role in pair formation and in species recognition. Determining the genetic basis of signal divergence will help to understand signal evolution by sexual selection and its role in the speciation process. An earlier study investigated quantitative trait locus for male courtship song carrier frequency (FRE) in Drosophila montana using microsatellite markers. We refined this study by adding to the linkage map markers for 10 candidate genes known to affect song production in Drosophila melanogaster. We also extended the analyses to additional song characters (pulse train length (PTL), pulse number (PN), interpulse interval, pulse length (PL) and cycle nu…

MaleCandidate geneX Chromosomeanimal structuresPeriod (gene)media_common.quotation_subjectGenome InsectMolecular Sequence DataQuantitative Trait LociGenes InsectQuantitative trait locusCourtshipSexual Behavior AnimalSpecies SpecificityGenetic linkageGeneticsAnimalsGenetics (clinical)X chromosomemedia_commonGeneticsbiologyCourtshipChromosome MappingGenetic Variationbiology.organism_classificationta1181DrosophilaOriginal ArticlefruitlessVocalization AnimalDrosophila melanogasterMicrosatellite RepeatsHeredity
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Genetic control of pheromones in Drosophila simulans. I. Ngbo, a locus on the second chromosome

1991

0016-6731 (Print) Journal Article; 7-Tricosene and 7-pentacosene are predominant hydrocarbons on the cuticle of both sexes in Drosophila simulans. The pheromonal role of 7-tricosene has been clearly established for conspecific males, while a synergistic effect for 7-pentacosene has been postulated. Interstrain variation for the production of both compounds is very marked, but similar for both sexes. The genetic basis of this polymorphism was investigated. A major role was found for the second chromosome, which controls the 7-tricosene:7-pentacosene ratio. The main locus involved in controlling this variation, Ngbo, was mapped to position 65.3 on the second chromosome. The production of 7-pe…

MaleChromatographyDrosophila/*genetics/metabolismGeneticGasAlkenes/*metabolismAnimalsChromosome MappingVariation (Genetics)FemalePolymorphismHydrocarbons/chemistryPheromones/*genetics/metabolism
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A single mutation alters production and discrimination of Drosophila sex pheromones

2005

0962-8452 (Print) Comparative Study Journal Article Research Support, Non-U.S. Gov't; The evolution of communication is a fundamental biological problem. The genetic control of the signal and its reception must be tightly coadapted, especially in inter-individual sexual communication. However, there is very little experimental evidence for tight genetic linkage connecting the emission of a signal and its reception. We found that a single genomic transposon inserted in the desatl gene of Drosophila melanogaster simultaneously affected the emission and the perception of sex-specific signals. This mutation greatly decreased the production of unsaturated hydrocarbons on the cuticle of mature fl…

MaleChromatographyMutation/geneticsBlottingHistocytochemistryMolecularGene ExpressionDNASex Attractants/*genetics/metabolismAnimal CommunicationDNA Transposable Elements/geneticsGene ComponentsFatty Acid Desaturases/*genetics/metabolismGasHydrocarbons/metabolismAnimalsDrosophila melanogaster/*geneticsFemaleSouthernSequence AnalysisCloning
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The visual orientation memory of Drosophila requires Foraging (PKG) upstream of Ignorant (RSK2) in ring neurons of the central complex

2012

Orientation and navigation in a complex environment requires path planning and recall to exert goal-driven behavior. Walking Drosophila flies possess a visual orientation memory for attractive targets which is localized in the central complex of the adult brain. Here we show that this type of working memory requires the cGMP-dependent protein kinase encoded by the foraging gene in just one type of ellipsoid-body ring neurons. Moreover, genetic and epistatic interaction studies provide evidence that Foraging functions upstream of the Ignorant Ribosomal-S6 Kinase 2, thus revealing a novel neuronal signaling pathway necessary for this type of memory in Drosophila.

MaleCognitive NeuroscienceGreen Fluorescent ProteinsForagingBrief CommunicationRibosomal Protein S6 Kinases 90-kDaStatistics NonparametricAnimals Genetically ModifiedCellular and Molecular NeuroscienceMemoryOrientationCyclic GMP-Dependent Protein KinasesAnimalsDrosophila ProteinsProtein kinase ADrosophilaNeuronsRegulation of gene expressionMemory DisordersCommunicationBehavior AnimalbiologyRecallWorking memorybusiness.industryfungiBrainbiology.organism_classificationNeuropsychology and Physiological PsychologyGene Expression RegulationDrosophilaFemaleSignal transductionbusinessNeurosciencePhotic StimulationDrosophila ProteinSignal TransductionLearning & Memory
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Cuticular hydrocarbons of Drosophila montana: geographic variation, sexual dimorphism and potential roles as pheromones.

2014

Abstract Sexual selection within populations can play an important role in speciation when divergence in mating signals and their corresponding preferences occur along different coevolutionary trajectories in different populations. In insects, one potential target of sexual selection is the blend of cuticular hydrocarbons (CHCs), which often show intra- and interspecific variation, sexual dimorphism and may act as pheromones. In Drosophila montana, a cold-adapted, circumboreal member of the Drosophila virilis species group, flies from different populations have been found to show significant premating isolation as well as variation in male mating signal (song) and female preference. While t…

MaleColoradoPhysiologymedia_common.quotation_subjectPopulationBiologyGas Chromatography-Mass SpectrometryPheromonesCourtshipAnimalsMatingSex AttractantseducationFinlandmedia_commoneducation.field_of_studyBritish ColumbiaEcologyMating Preference Animalbiology.organism_classificationHydrocarbonsSexual dimorphismDrosophila virilisAnimal CommunicationMate choiceEvolutionary biologyInsect ScienceSex pheromoneSexual selectionta1181DrosophilaFemaleEpidermisJournal of insect physiology
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Postnatal increases in axonal conduction velocity of an identified Drosophila interneuron require fast sodium, L-type calcium and shaker potassium ch…

2019

Abstract During early postnatal life, speed up of signal propagation through many central and peripheral neurons has been associated with an increase in axon diameter or/and myelination. Especially in unmyelinated axons postnatal adjustments of axonal membrane conductances is potentially a third mechanism but solid evidence is lacking. Here, we show that axonal action potential (AP) conduction velocity in the Drosophila giant fiber (GF) interneuron, which is required for fast long-distance signal conduction through the escape circuit, is increased by 80% during the first day of adult life. Genetic manipulations indicate that this postnatal increase in AP conduction velocity in the unmyelina…

MaleConfirmationaction potential propagationCalcium Channels L-Typepostnatal maturation2Neural ConductionAction PotentialsVoltage-Gated Sodium ChannelsDevelopmentgiant fiberAxonsvoltage-gated ion channels570 Life sciencesnervous systemInterneurons2.6LarvaShaker Superfamily of Potassium ChannelsAnimalsescapeinsectDrosophilaFemale570 Biowissenschaften
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Geographic distribution and inheritance of three cytoplasmic incompatibility types in Drosophila simulans.

1991

Abstract Wolbachia-like microorganisms have been implicated in unidirectional cytoplasmic incompatibility between strains of Drosophila simulans. Reduced egg eclosion occurs when females from uninfected strains (type W) are crossed with males from infected strains (type R). Here we characterize a third incompatibility type (type S) which is also correlated with the presence of Wolbachia-like microorganisms. Despite the fact that the symbionts cannot be morphologically distinguished, we observed complete bidirectional incompatibility between R and S strains. This indicates that the determinants of incompatibility are different in the two infected types. S/W incompatibility is unidirectional …

MaleCytoplasmVariation (Genetics)InvestigationsCrossesBiologyRickettsiaceaeRickettsiaceae/isolation & purificationRickettsiaceaeGeneticSymbiosisDrosophilidaeGenetic variationGeneticsAnimalsDrosophila (subgenus)SymbiosisCrosses GeneticGeneticsReproductionGenetic Variationbiology.organism_classificationDrosophila/*genetics/microbiologyDrosophilaFemaleWolbachiaRickettsialesCytoplasmic incompatibilityGenetics
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The Drosophila Cystoblast Differentiation Factor, benign gonial cell neoplasm, Is Related to DExH-box Proteins and Interacts Genetically With bag-of-…

2000

Abstract Selection of asymmetric cell fates can involve both intrinsic and extrinsic factors. Previously we have identified the bag-of-marbles (bam) gene as an intrinsic factor for cystoblast fate in Drosophila germline cells and shown that it requires active product from the benign gonial cell neoplasm (bgcn) gene. Here we present the cloning and characterization of bgcn. The predicted Bgcn protein is related to the DExH-box family of RNA-dependent helicases but lacks critical residues for ATPase and helicase functions. Expression of the bgcn gene is extremely limited in ovaries but, significantly, bgcn mRNA is expressed in a very limited number of germline cells, including the stem cells.…

MaleDNA ComplementaryMolecular Sequence DataGermlineGeneticsProtein biosynthesisAnimalsDrosophila ProteinsAmino Acid SequenceRNA MessengerCloning MolecularPromoter Regions GeneticGeneAllelesGene LibraryCloningGeneticsModels GeneticSequence Homology Amino AcidbiologyDNA HelicasesHelicasePhenotypeEnhancer Elements GeneticGerm CellsPhenotypeProtein Biosynthesisbiology.proteinInsect ProteinsDrosophilaFemaleStem cellRNA HelicasesDrosophila ProteinResearch ArticleGenetics
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