Search results for "EGF"
showing 10 items of 331 documents
Angiogenic and/or pro-apoptotic factors are shed from brain cells via extracellular vesicles
2008
We set a three-cell type coculture system in which neurons and astrocytes synergistically induce brain capillary endothelial cells to form a monolayer with permeability properties resembling those of the physiological blood-brain barrier (BBB) (Schiera et al., 2003; Schiera et al., 2005). On the basis of immunofluorescence, scanner electron microscopy and western blot analyses, we also suggested that both astrocytes and neurons in culture shed extracellular vesicles that contain FGF-2 and VEGF, as well as beta1-integrin, a membrane protein that can be considered a marker of shedding (Schiera et al, 2007; Proia et al., 2008). In addition, it was already known that transformed glial cells (ol…
Audzēja ekstracelulāro vezikulu loma mezenhimālo cilmes šūnu sekrēcijas profila izmaiņās
2017
Ekstarcelulārās vezikulas ir mazi pūslīši, kas tiek atbrīvoti no šūnām un izlaisti starpšūnu vidē. EV satur saimniekšūnu ģenētisko materiālu vai to daļas un caur tām viena šūna var nodot informācijas signālu otrai šūnai, tādā veidā ietekmējot to darbību. Arī vēža šūnas var atbrīvot šādas vezikulas un iedarboties uz blakusesošajām gan saimniekorganisma šūnām, gan pašām vēža šūnām. Caur šādu komunikāciju vēža šūnas var pārnest savu izmainīto ģenētisko materiālu uz normālajām šūnām un pārregulēt to darbības mehānismus, kas darbotos audzēja šūnu labā. Darba mērķis bija noskaidrot vai audzēja EV var izraisīt IL-8 un VEGF sekrēcijas izmaiņas ādas mezenhimālajās cilmes šūnās. Lai to noskaidrotu, I…
Impacto del miRNA-200c sobre el mecanismo de migración celular en el glioblastoma.Relación con los patrones de amplificación de EGFR.
2016
El Glioblastoma Multiforme (GB), la neoplasia primaria del SNC más importante en adultos, por su frecuencia y agresividad, se muestra como paradigmática de los procesos de infiltración y angiogénesis en las neoplasias. Su localización en el sistema nerviosos central, su alta capacidad invasiva y las consecuencias de una angiogénesis displasica hacen del GB una enfermedad neoplásicas altamente invalidante y de evolución fatal, en la que las distintas acciones terapéuticas se muestran ineficaces. Este trabajo realiza un estudio clínico, morfológico ,genético y epigenético de 46 casos de GB primarios. Se describe un grupo de miRNAs no descritos anteriormente en el GB. Se estableció la correlac…
Angiogenesis control in spine regeneration
2012
Abstract: The intervertebral disc (IVD) has a complex vascularisation pattern. While the nucleus pulposus is avascular, the annulus fibrosus as well as the endplates are vascularised. IVD degeneration is often accompanied, on the one hand, by blood vessel ingrowth into the nucleus pulposus and, on the other hand, by diminished vascularisation of the endplates. Tissue engineering of IVD, therefore, has to address the differences in the vascularisation of IVD compartments. This chapter summarises current knowledge about the mechanisms of angiogenesis and its physiological and pathological role in IVD biology. Different strategies to control angiogenesis are discussed in the chapter with examp…
Una nuova strategia ipocolesterolemizzante: ruolo del peptide EGF-A nell’inibizione in vitro dell’attività catalitica del mutante PCSK9-D374Y
2011
Parthenolide induces EGF receptor phosphorylation and superoxide anion production in MDA-MB231 breast cancer cells.
2013
The Value of Liquid Biopsies for Guiding Therapy Decisions in Non-small Cell Lung Cancer
2019
Cilvēka nātrija tauroholāta ko-transportējoša polipeptīda klonēšana un ekspresija zīdītāju šūnās
2015
Neskatoties uz to, ka ir izstrādāta droša un efektīva hepatīta B vakcīna, kā arī vesela rinda pret hepatīta B vīrusu (HBV) vērstu ķīmisku līdzekļu, pasaulē ir >350 miljonu hronisko HBV nēsātāju, no kuriem 1 miljons mirst katru gadu. Galvenais jaunu terapijas veidu izstrādāšanas kavēklis ir efektīvas HBV infekciju un replikāciju nodrošinošas in vitro modeļsistēmas trūkums. Pateicoties revolucionāram atklājumam par NTCP (sodium taurocholate cotransporting peptide) lomu HBV infekcijas procesā pašlaik tiek aktīvi izstrādātās jaunās HBV in vitro infekcijas modeļsistēmas. Mūsu darbā NTCP receptoru ekspresējošo šūnu iegūšanai tika izmantota efektīva Semliki Meža vīrusa (SFV) replikona ekspresijas …
Is Saffron Able to Prevent the Dysregulation of Retinal Cytokines Induced by Ocular Hypertension in Mice?
2021
Cytokine- and chemokine-mediated signalling is involved in the neuroinflammatory process that leads to retinal ganglion cell (RGC) damage in glaucoma. Substances with anti-inflammatory properties could decrease these cytokines and chemokines and thus prevent RGC death. The authors of this study analysed the anti-inflammatory effect of a hydrophilic saffron extract standardized to 3% crocin content, focusing on the regulation of cytokine and chemokine production, in a mouse model of unilateral laser-induced ocular hypertension (OHT). We demonstrated that following saffron treatment, most of the concentration of proinflammatory cytokines (IL-1β, IFN-γ, TNF-α, and IL-17), anti-inflammatory cyt…
Development of New Targeted Inulin Complex Nanoaggregates for siRNA Delivery in Antitumor Therapy.
2021
Here, a novel strategy of formulating efficient polymeric carriers based on the already described INU-IMI-DETA for gene material whose structural, functional, and biological properties can be modulated and improved was successfully investigated. In particular, two novel derivatives of INU-IMI-DETA graft copolymer were synthesized by chemical functionalisation with epidermal growth factor (EGF) or polyethylenglycol (PEG), named INU-IMI-DETA-EGF and INU-IMI-DETA-PEG, respectively, in order to improve the performance of already described “inulin complex nanoaggregates” (ICONs). The latter were thus prepared by appropriately mixing the two copolymers, by varying each component from 0 to 100 wt%…